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Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy
Photothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can sign...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182539/ https://www.ncbi.nlm.nih.gov/pubmed/35683721 http://dx.doi.org/10.3390/nano12111865 |
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author | Zhang, Weiwei Kuang, Zhao Song, Ping Li, Wanzhen Gui, Lin Tang, Chuchu Tao, Yugui Ge, Fei Zhu, Longbao |
author_facet | Zhang, Weiwei Kuang, Zhao Song, Ping Li, Wanzhen Gui, Lin Tang, Chuchu Tao, Yugui Ge, Fei Zhu, Longbao |
author_sort | Zhang, Weiwei |
collection | PubMed |
description | Photothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can significantly inhibit bacterial growth. We inhibited the growth of Staphylococcus aureus (S. aureus) by using an extremely low concentration of vancomycin (Van) and applied photothermal therapy with molybdenum disulfide (MoS(2)). This simple method used chitosan (CS) to synthesize fluorescein 5(6)-isothiocyanate (FITC)-labeled and Van-loaded MoS(2)-nanosheet hydrogels (MoS(2)-Van-FITC@CS). After modifying the surface, an extremely low concentration of Van could inhibit bacterial growth by trapping bacteria synergistically with the photothermal effects of MoS(2), while FITC labeled bacteria and chitosan hydrogels promoted wound healing. The results showed that MoS(2)-Van-FITC@CS nanosheets had a thickness of approximately 30 nm, indicating the successful synthesis of the nanosheets. The vitro antibacterial results showed that MoS(2)-Van-FITC with near-infrared irradiation significantly inhibited S. aureus growth, reaching an inhibition rate of 94.5% at nanoparticle concentrations of up to 100 µg/mL. Furthermore, MoS(2)-Van-FITC@CS could exert a healing effect on wounds in mice. Our results demonstrate that MoS(2)-Van-FITC@CS is biocompatible and can be used as a wound-healing agent. |
format | Online Article Text |
id | pubmed-9182539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91825392022-06-10 Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy Zhang, Weiwei Kuang, Zhao Song, Ping Li, Wanzhen Gui, Lin Tang, Chuchu Tao, Yugui Ge, Fei Zhu, Longbao Nanomaterials (Basel) Article Photothermal therapy has been widely used in the treatment of bacterial infections. However, the short photothermal effective radius of conventional nano-photothermal agents makes it difficult to achieve effective photothermal antibacterial activity. Therefore, improving composite targeting can significantly inhibit bacterial growth. We inhibited the growth of Staphylococcus aureus (S. aureus) by using an extremely low concentration of vancomycin (Van) and applied photothermal therapy with molybdenum disulfide (MoS(2)). This simple method used chitosan (CS) to synthesize fluorescein 5(6)-isothiocyanate (FITC)-labeled and Van-loaded MoS(2)-nanosheet hydrogels (MoS(2)-Van-FITC@CS). After modifying the surface, an extremely low concentration of Van could inhibit bacterial growth by trapping bacteria synergistically with the photothermal effects of MoS(2), while FITC labeled bacteria and chitosan hydrogels promoted wound healing. The results showed that MoS(2)-Van-FITC@CS nanosheets had a thickness of approximately 30 nm, indicating the successful synthesis of the nanosheets. The vitro antibacterial results showed that MoS(2)-Van-FITC with near-infrared irradiation significantly inhibited S. aureus growth, reaching an inhibition rate of 94.5% at nanoparticle concentrations of up to 100 µg/mL. Furthermore, MoS(2)-Van-FITC@CS could exert a healing effect on wounds in mice. Our results demonstrate that MoS(2)-Van-FITC@CS is biocompatible and can be used as a wound-healing agent. MDPI 2022-05-30 /pmc/articles/PMC9182539/ /pubmed/35683721 http://dx.doi.org/10.3390/nano12111865 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Weiwei Kuang, Zhao Song, Ping Li, Wanzhen Gui, Lin Tang, Chuchu Tao, Yugui Ge, Fei Zhu, Longbao Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title_full | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title_fullStr | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title_full_unstemmed | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title_short | Synthesis of a Two-Dimensional Molybdenum Disulfide Nanosheet and Ultrasensitive Trapping of Staphylococcus Aureus for Enhanced Photothermal and Antibacterial Wound-Healing Therapy |
title_sort | synthesis of a two-dimensional molybdenum disulfide nanosheet and ultrasensitive trapping of staphylococcus aureus for enhanced photothermal and antibacterial wound-healing therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182539/ https://www.ncbi.nlm.nih.gov/pubmed/35683721 http://dx.doi.org/10.3390/nano12111865 |
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