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A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.)
The high-affinity potassium transporters (HKT) mediate K(+)-Na(+) homeostasis in plants. However, the function of enhancing low-potassium tolerance in sweet potato [Ipomoea batatas (L.) Lam.] remains unrevealed. In this study, a novel HKT transporter homolog IbHKT-like gene was cloned from sweet pot...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182616/ https://www.ncbi.nlm.nih.gov/pubmed/35684162 http://dx.doi.org/10.3390/plants11111389 |
Sumario: | The high-affinity potassium transporters (HKT) mediate K(+)-Na(+) homeostasis in plants. However, the function of enhancing low-potassium tolerance in sweet potato [Ipomoea batatas (L.) Lam.] remains unrevealed. In this study, a novel HKT transporter homolog IbHKT-like gene was cloned from sweet potato, which was significantly induced by potassium deficiency stress. IbHKT-like overexpressing transgenic roots were obtained from a sweet potato cultivar Xuzishu8 using an Agrobacterium rhizogenes-mediated root transgenic system in vivo. Compared with the CK, whose root cells did not overexpress the IbHKT-like gene, overexpression of the IbHKT-like gene protected cell ultrastructure from damage, and transgenic root meristem cells had intact mitochondria, endoplasmic reticulum, and Golgi dictyosomes. The steady-state K(+) influx increased by 2.2 times in transgenic root meristem cells. Overexpression of the IbHKT-like gene also improved potassium content in the whole plant, which increased by 63.8% compared with the CK plants. These results could imply that the IbHKT-like gene, as a high-affinity potassium transporter gene, may play an important role in potassium deficiency stress responses. |
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