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Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells

Recent and growing literature has reported that oleuropein (OLE), the main polyphenol in olive leaf extract, inhibits tumor cell proliferation and reduces the invasiveness properties of cancer cells; therefore, OLE may play a significant role in the development of new drugs for cancer treatment. The...

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Autores principales: Bossio, Sabrina, Perri, Anna, Malivindi, Rocco, Giordano, Francesca, Rago, Vittoria, Mirabelli, Maria, Salatino, Alessandro, Brunetti, Antonio, Greco, Emanuela Alessandra, Aversa, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182631/
https://www.ncbi.nlm.nih.gov/pubmed/35684123
http://dx.doi.org/10.3390/nu14112323
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author Bossio, Sabrina
Perri, Anna
Malivindi, Rocco
Giordano, Francesca
Rago, Vittoria
Mirabelli, Maria
Salatino, Alessandro
Brunetti, Antonio
Greco, Emanuela Alessandra
Aversa, Antonio
author_facet Bossio, Sabrina
Perri, Anna
Malivindi, Rocco
Giordano, Francesca
Rago, Vittoria
Mirabelli, Maria
Salatino, Alessandro
Brunetti, Antonio
Greco, Emanuela Alessandra
Aversa, Antonio
author_sort Bossio, Sabrina
collection PubMed
description Recent and growing literature has reported that oleuropein (OLE), the main polyphenol in olive leaf extract, inhibits tumor cell proliferation and reduces the invasiveness properties of cancer cells; therefore, OLE may play a significant role in the development of new drugs for cancer treatment. These antineoplastic properties have been reported in many experimental cancer models, but the effect of OLE on seminoma cells is yet to be evaluated. In the present study, we demonstrate, for the first time, that OLE reduces cell viability in both intra- and extragonadal TCAM-2 and SEM-1 seminoma cells, respectively, in a dose-dependent manner. As shown by Western-blot analysis, OLE exposure reduced cyclin-D1 expression and upregulated p21(Cip/WAF1), concomitantly affecting the upstream pathway of NF-κB, leading to the reduction of its nuclear content, thereby suggesting that OLE could modulate cell-cycle regulators by inhibiting NF-κB. Moreover, Annexin V staining revealed that OLE induced apoptosis in cancer cells and upregulated the pro-apoptotic factor BAX. Through wound-healing scratch and transmigration assays, we also demonstrated that OLE significantly reduced the migration and motility of TCAM-2 and SEM-1 cells, and downregulated the expression of TGFβ-1, which is known to be the main pro-fibrotic factor involved in the acquisition of the migratory and invasive properties of cancer cells. Collectively, our results indicate that OLE reduces seminoma cell proliferation, promotes apoptosis, and counteracts cell migration and motility. Further studies are needed to explore the molecular mechanisms underlying these observed effects.
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spelling pubmed-91826312022-06-10 Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells Bossio, Sabrina Perri, Anna Malivindi, Rocco Giordano, Francesca Rago, Vittoria Mirabelli, Maria Salatino, Alessandro Brunetti, Antonio Greco, Emanuela Alessandra Aversa, Antonio Nutrients Article Recent and growing literature has reported that oleuropein (OLE), the main polyphenol in olive leaf extract, inhibits tumor cell proliferation and reduces the invasiveness properties of cancer cells; therefore, OLE may play a significant role in the development of new drugs for cancer treatment. These antineoplastic properties have been reported in many experimental cancer models, but the effect of OLE on seminoma cells is yet to be evaluated. In the present study, we demonstrate, for the first time, that OLE reduces cell viability in both intra- and extragonadal TCAM-2 and SEM-1 seminoma cells, respectively, in a dose-dependent manner. As shown by Western-blot analysis, OLE exposure reduced cyclin-D1 expression and upregulated p21(Cip/WAF1), concomitantly affecting the upstream pathway of NF-κB, leading to the reduction of its nuclear content, thereby suggesting that OLE could modulate cell-cycle regulators by inhibiting NF-κB. Moreover, Annexin V staining revealed that OLE induced apoptosis in cancer cells and upregulated the pro-apoptotic factor BAX. Through wound-healing scratch and transmigration assays, we also demonstrated that OLE significantly reduced the migration and motility of TCAM-2 and SEM-1 cells, and downregulated the expression of TGFβ-1, which is known to be the main pro-fibrotic factor involved in the acquisition of the migratory and invasive properties of cancer cells. Collectively, our results indicate that OLE reduces seminoma cell proliferation, promotes apoptosis, and counteracts cell migration and motility. Further studies are needed to explore the molecular mechanisms underlying these observed effects. MDPI 2022-05-31 /pmc/articles/PMC9182631/ /pubmed/35684123 http://dx.doi.org/10.3390/nu14112323 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bossio, Sabrina
Perri, Anna
Malivindi, Rocco
Giordano, Francesca
Rago, Vittoria
Mirabelli, Maria
Salatino, Alessandro
Brunetti, Antonio
Greco, Emanuela Alessandra
Aversa, Antonio
Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title_full Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title_fullStr Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title_full_unstemmed Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title_short Oleuropein Counteracts Both the Proliferation and Migration of Intra- and Extragonadal Seminoma Cells
title_sort oleuropein counteracts both the proliferation and migration of intra- and extragonadal seminoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182631/
https://www.ncbi.nlm.nih.gov/pubmed/35684123
http://dx.doi.org/10.3390/nu14112323
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