Cargando…
Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources
The objective of the present study was the evaluation of cytokine patterns in terms of TNF-α, IL-10, IL-6, and IL-1β secretion in peripheral blood mononuclear cell (PBMC) supernatants isolated from blood of children affected by generalized epilepsy and treated in vitro with myofibrillar, sarcoplasmi...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182632/ https://www.ncbi.nlm.nih.gov/pubmed/35684043 http://dx.doi.org/10.3390/nu14112243 |
_version_ | 1784724082929958912 |
---|---|
author | Cilberti, Maria Giovanna Santillo, Antonella Polito, Anna N. Messina, Giovanni della Malva, Antonella Caroprese, Mariangela Sevi, Agostino Albenzio, Marzia |
author_facet | Cilberti, Maria Giovanna Santillo, Antonella Polito, Anna N. Messina, Giovanni della Malva, Antonella Caroprese, Mariangela Sevi, Agostino Albenzio, Marzia |
author_sort | Cilberti, Maria Giovanna |
collection | PubMed |
description | The objective of the present study was the evaluation of cytokine patterns in terms of TNF-α, IL-10, IL-6, and IL-1β secretion in peripheral blood mononuclear cell (PBMC) supernatants isolated from blood of children affected by generalized epilepsy and treated in vitro with myofibrillar, sarcoplasmic, and total protein fractions of meat and fish sources. Children with generalized epilepsy (EC group, n = 16) and children without any clinical signs of disease, representing a control group (CC group n = 16), were recruited at the Complex Structure of Neuropsychiatry Childhood-Adolescence of Policlinico Riuniti (Foggia, Italy). Myofibrillar (MYO), sarcoplasmic (SA), and total (TOT) protein fractions were obtained from longissimus thoracis muscle of beef (BF) and lamb (LA); from pectoralis muscle of chicken (CH); and from dorsal white muscle of sole (Solea solea, SO), European hake (Merluccius merluccius, EH), and sea bass fish (Dicentrarchus labrax, SB), respectively. PBMCs were isolated from peripheral blood of EC and CC groups, and an in vitro stimulation in the presence of 100 μg/mL for each protein fraction from different meat sources was performed. Data were classified according to three different levels of cytokines produced from the EC group relative to the CC group. TNF-α, IL-10, and IL-6 levels were not affected by different meat fractions and meat sources; on the contrary, IL-1β levels were found to be significantly affected by the tested proteins fractions, as well as different meat sources, in high-level cytokine group. On average, the protein fractions obtained from LB, BF, and CH meat sources showed a higher level of IL-1β than the protein fractions obtained from EH and SB fish samples. When all cytokine classes were analyzed, on average, a significant effect was observed for IL-10, IL-1β, and TNF-α. Data obtained in the present study evidence that the nutritional strategy based on protein from fish and meat sources may modulate the immunological cytokine pattern of infants with generalized epilepsy. |
format | Online Article Text |
id | pubmed-9182632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91826322022-06-10 Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources Cilberti, Maria Giovanna Santillo, Antonella Polito, Anna N. Messina, Giovanni della Malva, Antonella Caroprese, Mariangela Sevi, Agostino Albenzio, Marzia Nutrients Article The objective of the present study was the evaluation of cytokine patterns in terms of TNF-α, IL-10, IL-6, and IL-1β secretion in peripheral blood mononuclear cell (PBMC) supernatants isolated from blood of children affected by generalized epilepsy and treated in vitro with myofibrillar, sarcoplasmic, and total protein fractions of meat and fish sources. Children with generalized epilepsy (EC group, n = 16) and children without any clinical signs of disease, representing a control group (CC group n = 16), were recruited at the Complex Structure of Neuropsychiatry Childhood-Adolescence of Policlinico Riuniti (Foggia, Italy). Myofibrillar (MYO), sarcoplasmic (SA), and total (TOT) protein fractions were obtained from longissimus thoracis muscle of beef (BF) and lamb (LA); from pectoralis muscle of chicken (CH); and from dorsal white muscle of sole (Solea solea, SO), European hake (Merluccius merluccius, EH), and sea bass fish (Dicentrarchus labrax, SB), respectively. PBMCs were isolated from peripheral blood of EC and CC groups, and an in vitro stimulation in the presence of 100 μg/mL for each protein fraction from different meat sources was performed. Data were classified according to three different levels of cytokines produced from the EC group relative to the CC group. TNF-α, IL-10, and IL-6 levels were not affected by different meat fractions and meat sources; on the contrary, IL-1β levels were found to be significantly affected by the tested proteins fractions, as well as different meat sources, in high-level cytokine group. On average, the protein fractions obtained from LB, BF, and CH meat sources showed a higher level of IL-1β than the protein fractions obtained from EH and SB fish samples. When all cytokine classes were analyzed, on average, a significant effect was observed for IL-10, IL-1β, and TNF-α. Data obtained in the present study evidence that the nutritional strategy based on protein from fish and meat sources may modulate the immunological cytokine pattern of infants with generalized epilepsy. MDPI 2022-05-27 /pmc/articles/PMC9182632/ /pubmed/35684043 http://dx.doi.org/10.3390/nu14112243 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cilberti, Maria Giovanna Santillo, Antonella Polito, Anna N. Messina, Giovanni della Malva, Antonella Caroprese, Mariangela Sevi, Agostino Albenzio, Marzia Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title | Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title_full | Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title_fullStr | Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title_full_unstemmed | Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title_short | Cytokine Pattern of Peripheral Blood Mononuclear Cells Isolated from Children Affected by Generalized Epilepsy Treated with Different Protein Fractions of Meat Sources |
title_sort | cytokine pattern of peripheral blood mononuclear cells isolated from children affected by generalized epilepsy treated with different protein fractions of meat sources |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182632/ https://www.ncbi.nlm.nih.gov/pubmed/35684043 http://dx.doi.org/10.3390/nu14112243 |
work_keys_str_mv | AT cilbertimariagiovanna cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT santilloantonella cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT politoannan cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT messinagiovanni cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT dellamalvaantonella cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT caropresemariangela cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT seviagostino cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources AT albenziomarzia cytokinepatternofperipheralbloodmononuclearcellsisolatedfromchildrenaffectedbygeneralizedepilepsytreatedwithdifferentproteinfractionsofmeatsources |