Cargando…

Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars

Grapevine collections play an important role, especially in the study of viruses and virus-like pathogens. In 2009, after an initial ELISA screening for eight viruses (arabis mosaic virus, grapevine fanleaf virus, grapevine fleck virus, grapevine leafroll-associated viruses 1, 2, and 3, and grapevin...

Descripción completa

Detalles Bibliográficos
Autores principales: Vončina, Darko, Diaz-Lara, Alfredo, Preiner, Darko, Al Rwahnih, Maher, Stevens, Kristian, Jurić, Snježana, Malenica, Nenad, Šimon, Silvio, Meng, Baozhong, Maletić, Edi, Fulgosi, Hrvoje, Cvjetković, Bogdan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182833/
https://www.ncbi.nlm.nih.gov/pubmed/35684258
http://dx.doi.org/10.3390/plants11111485
_version_ 1784724135652360192
author Vončina, Darko
Diaz-Lara, Alfredo
Preiner, Darko
Al Rwahnih, Maher
Stevens, Kristian
Jurić, Snježana
Malenica, Nenad
Šimon, Silvio
Meng, Baozhong
Maletić, Edi
Fulgosi, Hrvoje
Cvjetković, Bogdan
author_facet Vončina, Darko
Diaz-Lara, Alfredo
Preiner, Darko
Al Rwahnih, Maher
Stevens, Kristian
Jurić, Snježana
Malenica, Nenad
Šimon, Silvio
Meng, Baozhong
Maletić, Edi
Fulgosi, Hrvoje
Cvjetković, Bogdan
author_sort Vončina, Darko
collection PubMed
description Grapevine collections play an important role, especially in the study of viruses and virus-like pathogens. In 2009, after an initial ELISA screening for eight viruses (arabis mosaic virus, grapevine fanleaf virus, grapevine fleck virus, grapevine leafroll-associated viruses 1, 2, and 3, and grapevine viruses A and B), a collection of 368 grapevine accessions representing 14 different Croatian autochthonous cultivars and containing single or mixed infection of viruses was established to further characterize the viral pathogens. Subsequently, Western blot, RT-PCR, cloning, and sequencing revealed that grapevine rupestris stem pitting-associated virus was frequently found in accessions of the collection, with isolates showing substantial genetic diversity in the helicase and coat protein regions. High-throughput sequencing of 22 grapevine accessions provides additional insight into the viruses and viroids present in the collection and confirms the fact that Croatian autochthonous grapevine cultivars have high infection rates and high virome diversity. The recent spread of “flavescence dorée” phytoplasma in Europe has not spared the collection. After the first symptoms observed in 2020 and 2021, the presence of phytoplasma was confirmed by LAMP in six grapevine accessions and some of them were lost. Single or multiple viruses and viroids, as well as own rooted grapevines in the collection, make the plants susceptible to various abiotic factors, which, together with the recent occurrence of “flavescence dorée”, makes the maintenance of the collection a challenge. Future efforts will be directed towards renewing the collection, as 56% of the original collection has been lost in the last 13 years.
format Online
Article
Text
id pubmed-9182833
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91828332022-06-10 Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars Vončina, Darko Diaz-Lara, Alfredo Preiner, Darko Al Rwahnih, Maher Stevens, Kristian Jurić, Snježana Malenica, Nenad Šimon, Silvio Meng, Baozhong Maletić, Edi Fulgosi, Hrvoje Cvjetković, Bogdan Plants (Basel) Article Grapevine collections play an important role, especially in the study of viruses and virus-like pathogens. In 2009, after an initial ELISA screening for eight viruses (arabis mosaic virus, grapevine fanleaf virus, grapevine fleck virus, grapevine leafroll-associated viruses 1, 2, and 3, and grapevine viruses A and B), a collection of 368 grapevine accessions representing 14 different Croatian autochthonous cultivars and containing single or mixed infection of viruses was established to further characterize the viral pathogens. Subsequently, Western blot, RT-PCR, cloning, and sequencing revealed that grapevine rupestris stem pitting-associated virus was frequently found in accessions of the collection, with isolates showing substantial genetic diversity in the helicase and coat protein regions. High-throughput sequencing of 22 grapevine accessions provides additional insight into the viruses and viroids present in the collection and confirms the fact that Croatian autochthonous grapevine cultivars have high infection rates and high virome diversity. The recent spread of “flavescence dorée” phytoplasma in Europe has not spared the collection. After the first symptoms observed in 2020 and 2021, the presence of phytoplasma was confirmed by LAMP in six grapevine accessions and some of them were lost. Single or multiple viruses and viroids, as well as own rooted grapevines in the collection, make the plants susceptible to various abiotic factors, which, together with the recent occurrence of “flavescence dorée”, makes the maintenance of the collection a challenge. Future efforts will be directed towards renewing the collection, as 56% of the original collection has been lost in the last 13 years. MDPI 2022-05-31 /pmc/articles/PMC9182833/ /pubmed/35684258 http://dx.doi.org/10.3390/plants11111485 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vončina, Darko
Diaz-Lara, Alfredo
Preiner, Darko
Al Rwahnih, Maher
Stevens, Kristian
Jurić, Snježana
Malenica, Nenad
Šimon, Silvio
Meng, Baozhong
Maletić, Edi
Fulgosi, Hrvoje
Cvjetković, Bogdan
Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title_full Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title_fullStr Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title_full_unstemmed Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title_short Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
title_sort virus and virus-like pathogens in the grapevine virus collection of croatian autochthonous grapevine cultivars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182833/
https://www.ncbi.nlm.nih.gov/pubmed/35684258
http://dx.doi.org/10.3390/plants11111485
work_keys_str_mv AT voncinadarko virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT diazlaraalfredo virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT preinerdarko virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT alrwahnihmaher virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT stevenskristian virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT juricsnjezana virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT malenicanenad virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT simonsilvio virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT mengbaozhong virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT maleticedi virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT fulgosihrvoje virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars
AT cvjetkovicbogdan virusandviruslikepathogensinthegrapevineviruscollectionofcroatianautochthonousgrapevinecultivars