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Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions

The Typha genus comprises plant species extensively studied for phytoremediation processes. Recently, Pseudomonas rhodesiae GRC140, an IAA-producing bacterium, was isolated from Typha latifolia roots. This bacterium stimulates the emergence of lateral roots of Arabidopsis thaliana in the presence an...

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Autores principales: Rolón-Cárdenas, Gisela Adelina, Martínez-Martínez, Joana Guadalupe, Arvizu-Gómez, Jackeline Lizzeta, Soria-Guerra, Ruth Elena, Alfaro-De la Torre, Ma. Catalina, Alatorre-Cobos, Fulgencio, Rubio-Santiago, Jesús, González-Balderas, Regina de Montserrat, Carranza-Álvarez, Candy, Macías-Pérez, José Roberto, Aldaba-Muruato, Liseth Rubí, Hernández-Morales, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9183143/
https://www.ncbi.nlm.nih.gov/pubmed/35684220
http://dx.doi.org/10.3390/plants11111447
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author Rolón-Cárdenas, Gisela Adelina
Martínez-Martínez, Joana Guadalupe
Arvizu-Gómez, Jackeline Lizzeta
Soria-Guerra, Ruth Elena
Alfaro-De la Torre, Ma. Catalina
Alatorre-Cobos, Fulgencio
Rubio-Santiago, Jesús
González-Balderas, Regina de Montserrat
Carranza-Álvarez, Candy
Macías-Pérez, José Roberto
Aldaba-Muruato, Liseth Rubí
Hernández-Morales, Alejandro
author_facet Rolón-Cárdenas, Gisela Adelina
Martínez-Martínez, Joana Guadalupe
Arvizu-Gómez, Jackeline Lizzeta
Soria-Guerra, Ruth Elena
Alfaro-De la Torre, Ma. Catalina
Alatorre-Cobos, Fulgencio
Rubio-Santiago, Jesús
González-Balderas, Regina de Montserrat
Carranza-Álvarez, Candy
Macías-Pérez, José Roberto
Aldaba-Muruato, Liseth Rubí
Hernández-Morales, Alejandro
author_sort Rolón-Cárdenas, Gisela Adelina
collection PubMed
description The Typha genus comprises plant species extensively studied for phytoremediation processes. Recently, Pseudomonas rhodesiae GRC140, an IAA-producing bacterium, was isolated from Typha latifolia roots. This bacterium stimulates the emergence of lateral roots of Arabidopsis thaliana in the presence and absence of cadmium. However, the bacterial influence on cadmium accumulation by the plant has not been determined. Moreover, the P. rhodesiae GRC140 effect in Cd phytoextraction by T. latifolia remains poorly understood. In this work, an axenic hydroponic culture of T. latifolia was established. The plants were used to evaluate the effects of cadmium stress in axenic plants and determine the effects of P. rhodesiae GRC140 and exogenous indole acetic acid (IAA) on Cd tolerance and Cd uptake by T. latifolia. Biomass production, total chlorophyll content, root electrolyte leakage, catalase activity, total glutathione, and Cd content were determined. The results showed that Cd reduces shoot biomass and increases total glutathione and Cd content in a dose-dependent manner in root tissues. Furthermore, P. rhodesiae GRC140 increased Cd translocation to the shoots, while IAA increased the Cd accumulation in plant roots, indicating that both treatments increase Cd removal by T. latifolia plants. These results indicate that axenic plants in hydroponic systems are adequate to evaluate the Cd effects in plants and suggest that T. latifolia phytoextraction abilities could be improved by P. rhodesiae GRC140 and exogenous IAA application.
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spelling pubmed-91831432022-06-10 Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions Rolón-Cárdenas, Gisela Adelina Martínez-Martínez, Joana Guadalupe Arvizu-Gómez, Jackeline Lizzeta Soria-Guerra, Ruth Elena Alfaro-De la Torre, Ma. Catalina Alatorre-Cobos, Fulgencio Rubio-Santiago, Jesús González-Balderas, Regina de Montserrat Carranza-Álvarez, Candy Macías-Pérez, José Roberto Aldaba-Muruato, Liseth Rubí Hernández-Morales, Alejandro Plants (Basel) Article The Typha genus comprises plant species extensively studied for phytoremediation processes. Recently, Pseudomonas rhodesiae GRC140, an IAA-producing bacterium, was isolated from Typha latifolia roots. This bacterium stimulates the emergence of lateral roots of Arabidopsis thaliana in the presence and absence of cadmium. However, the bacterial influence on cadmium accumulation by the plant has not been determined. Moreover, the P. rhodesiae GRC140 effect in Cd phytoextraction by T. latifolia remains poorly understood. In this work, an axenic hydroponic culture of T. latifolia was established. The plants were used to evaluate the effects of cadmium stress in axenic plants and determine the effects of P. rhodesiae GRC140 and exogenous indole acetic acid (IAA) on Cd tolerance and Cd uptake by T. latifolia. Biomass production, total chlorophyll content, root electrolyte leakage, catalase activity, total glutathione, and Cd content were determined. The results showed that Cd reduces shoot biomass and increases total glutathione and Cd content in a dose-dependent manner in root tissues. Furthermore, P. rhodesiae GRC140 increased Cd translocation to the shoots, while IAA increased the Cd accumulation in plant roots, indicating that both treatments increase Cd removal by T. latifolia plants. These results indicate that axenic plants in hydroponic systems are adequate to evaluate the Cd effects in plants and suggest that T. latifolia phytoextraction abilities could be improved by P. rhodesiae GRC140 and exogenous IAA application. MDPI 2022-05-29 /pmc/articles/PMC9183143/ /pubmed/35684220 http://dx.doi.org/10.3390/plants11111447 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rolón-Cárdenas, Gisela Adelina
Martínez-Martínez, Joana Guadalupe
Arvizu-Gómez, Jackeline Lizzeta
Soria-Guerra, Ruth Elena
Alfaro-De la Torre, Ma. Catalina
Alatorre-Cobos, Fulgencio
Rubio-Santiago, Jesús
González-Balderas, Regina de Montserrat
Carranza-Álvarez, Candy
Macías-Pérez, José Roberto
Aldaba-Muruato, Liseth Rubí
Hernández-Morales, Alejandro
Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title_full Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title_fullStr Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title_full_unstemmed Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title_short Enhanced Cd-Accumulation in Typha latifolia by Interaction with Pseudomonas rhodesiae GRC140 under Axenic Hydroponic Conditions
title_sort enhanced cd-accumulation in typha latifolia by interaction with pseudomonas rhodesiae grc140 under axenic hydroponic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9183143/
https://www.ncbi.nlm.nih.gov/pubmed/35684220
http://dx.doi.org/10.3390/plants11111447
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