Cargando…

Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes

Origanum majorana L. is a widely used medicinal plant; its distilled oil and preparations are extensively utilised in the phytotherapy and food industries. The objective of this study is to evaluate the extracts and the essential oil (EO) of Origanum majorana L, and its monoterpenes for antimicrobia...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghazal, Tasneem Sultan Abu, Schelz, Zsuzsanna, Vidács, Lívia, Szemerédi, Nikoletta, Veres, Katalin, Spengler, Gabriella, Hohmann, Judit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9183178/
https://www.ncbi.nlm.nih.gov/pubmed/35684205
http://dx.doi.org/10.3390/plants11111432
_version_ 1784724226268200960
author Ghazal, Tasneem Sultan Abu
Schelz, Zsuzsanna
Vidács, Lívia
Szemerédi, Nikoletta
Veres, Katalin
Spengler, Gabriella
Hohmann, Judit
author_facet Ghazal, Tasneem Sultan Abu
Schelz, Zsuzsanna
Vidács, Lívia
Szemerédi, Nikoletta
Veres, Katalin
Spengler, Gabriella
Hohmann, Judit
author_sort Ghazal, Tasneem Sultan Abu
collection PubMed
description Origanum majorana L. is a widely used medicinal plant; its distilled oil and preparations are extensively utilised in the phytotherapy and food industries. The objective of this study is to evaluate the extracts and the essential oil (EO) of Origanum majorana L, and its monoterpenes for antimicrobial, bacterial multidrug resistance reversing, and biofilm formation inhibitory potency. The composition of EO and n-hexane extract was characterized by GC-MS. In the essential oil terpinen-4-ol (24.92%), trans-sabinene hydrate (25.18%), γ-terpinene (6.48%), cis-sabinene hydrate (5.44%), p-cymene (4.72%), sabinene (4.53%), α-terpineol (4.43%), and α-terpinene (3.00%) were found as the main constituents while trans-sabinene hydrate (1.43%), and terpinen-4-ol (0.19%) were detected in the n-hexane extract besides a series of hydrocarbons. The antibacterial activity of EO and terpinen-4-ol, α-terpinene, and linalool was also assessed against sensitive and drug-resistant S. aureus, and E. coli strains with MIC values of 0.125–0.250% and 30–61 µM, respectively. In the efflux pump (EP) inhibitory assay, made by the ethidium bromide accumulation method in E. coli ATCC 25922, and AG100 and S. aureus ATCC 25923, and MRSA ATCC 43300 strains, EO exhibited substantial activity, especially in the E. coli ATCC 25922 strain. Among the EO constituents, only sabinene was an EP inhibitor in sensitive Escherichia strain. In the case of S. aureus strains, EO and sabinene hydrate exhibited moderate potency on the drug-resistant phenotype. The antibiofilm effects of the samples were tested by crystal violet staining at sub-MIC concentration. γ-Terpinene, terpinen-4-ol, sabinene, sabinene hydrate and linalool were found to be effective inhibitors of biofilm formation (inhibition 36–86%) on E. coli ATCC 25922 and S. aureus MRSA ATCC 43300, while EO was ineffective on these strains. In contrast to this, biofilms formed by E. coli AG100 and S. aureus ATCC 25923 were significantly inhibited by the EO; however, it was not affected by any of the monoterpenes. This observation suggests that the antibiofilm effect might be altered by the synergism between the components of the essential oil.
format Online
Article
Text
id pubmed-9183178
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91831782022-06-10 Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes Ghazal, Tasneem Sultan Abu Schelz, Zsuzsanna Vidács, Lívia Szemerédi, Nikoletta Veres, Katalin Spengler, Gabriella Hohmann, Judit Plants (Basel) Article Origanum majorana L. is a widely used medicinal plant; its distilled oil and preparations are extensively utilised in the phytotherapy and food industries. The objective of this study is to evaluate the extracts and the essential oil (EO) of Origanum majorana L, and its monoterpenes for antimicrobial, bacterial multidrug resistance reversing, and biofilm formation inhibitory potency. The composition of EO and n-hexane extract was characterized by GC-MS. In the essential oil terpinen-4-ol (24.92%), trans-sabinene hydrate (25.18%), γ-terpinene (6.48%), cis-sabinene hydrate (5.44%), p-cymene (4.72%), sabinene (4.53%), α-terpineol (4.43%), and α-terpinene (3.00%) were found as the main constituents while trans-sabinene hydrate (1.43%), and terpinen-4-ol (0.19%) were detected in the n-hexane extract besides a series of hydrocarbons. The antibacterial activity of EO and terpinen-4-ol, α-terpinene, and linalool was also assessed against sensitive and drug-resistant S. aureus, and E. coli strains with MIC values of 0.125–0.250% and 30–61 µM, respectively. In the efflux pump (EP) inhibitory assay, made by the ethidium bromide accumulation method in E. coli ATCC 25922, and AG100 and S. aureus ATCC 25923, and MRSA ATCC 43300 strains, EO exhibited substantial activity, especially in the E. coli ATCC 25922 strain. Among the EO constituents, only sabinene was an EP inhibitor in sensitive Escherichia strain. In the case of S. aureus strains, EO and sabinene hydrate exhibited moderate potency on the drug-resistant phenotype. The antibiofilm effects of the samples were tested by crystal violet staining at sub-MIC concentration. γ-Terpinene, terpinen-4-ol, sabinene, sabinene hydrate and linalool were found to be effective inhibitors of biofilm formation (inhibition 36–86%) on E. coli ATCC 25922 and S. aureus MRSA ATCC 43300, while EO was ineffective on these strains. In contrast to this, biofilms formed by E. coli AG100 and S. aureus ATCC 25923 were significantly inhibited by the EO; however, it was not affected by any of the monoterpenes. This observation suggests that the antibiofilm effect might be altered by the synergism between the components of the essential oil. MDPI 2022-05-27 /pmc/articles/PMC9183178/ /pubmed/35684205 http://dx.doi.org/10.3390/plants11111432 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghazal, Tasneem Sultan Abu
Schelz, Zsuzsanna
Vidács, Lívia
Szemerédi, Nikoletta
Veres, Katalin
Spengler, Gabriella
Hohmann, Judit
Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title_full Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title_fullStr Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title_full_unstemmed Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title_short Antimicrobial, Multidrug Resistance Reversal and Biofilm Formation Inhibitory Effect of Origanum majorana Extracts, Essential Oil and Monoterpenes
title_sort antimicrobial, multidrug resistance reversal and biofilm formation inhibitory effect of origanum majorana extracts, essential oil and monoterpenes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9183178/
https://www.ncbi.nlm.nih.gov/pubmed/35684205
http://dx.doi.org/10.3390/plants11111432
work_keys_str_mv AT ghazaltasneemsultanabu antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT schelzzsuzsanna antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT vidacslivia antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT szemeredinikoletta antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT vereskatalin antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT spenglergabriella antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes
AT hohmannjudit antimicrobialmultidrugresistancereversalandbiofilmformationinhibitoryeffectoforiganummajoranaextractsessentialoilandmonoterpenes