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Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling

In the present study, we investigated the cytotoxic effects of Athyrium hohenackerianum ethanolic extract (AHEE) on the proliferation of breast, lung, and colon cancer cells. The AHEE was tested for its effect on the progression of the cell cycle, followed by induction of apoptosis determination by...

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Autores principales: Elasbali, Abdelbaset Mohamed, Al-Soud, Waleed Abu, Al-Oanzi, Ziad H., Qanash, Husam, Alharbi, Bandar, Binsaleh, Naif K., Alreshidi, Mousa, Patel, Mitesh, Adnan, Mohd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184205/
https://www.ncbi.nlm.nih.gov/pubmed/35692581
http://dx.doi.org/10.1155/2022/2055773
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author Elasbali, Abdelbaset Mohamed
Al-Soud, Waleed Abu
Al-Oanzi, Ziad H.
Qanash, Husam
Alharbi, Bandar
Binsaleh, Naif K.
Alreshidi, Mousa
Patel, Mitesh
Adnan, Mohd
author_facet Elasbali, Abdelbaset Mohamed
Al-Soud, Waleed Abu
Al-Oanzi, Ziad H.
Qanash, Husam
Alharbi, Bandar
Binsaleh, Naif K.
Alreshidi, Mousa
Patel, Mitesh
Adnan, Mohd
author_sort Elasbali, Abdelbaset Mohamed
collection PubMed
description In the present study, we investigated the cytotoxic effects of Athyrium hohenackerianum ethanolic extract (AHEE) on the proliferation of breast, lung, and colon cancer cells. The AHEE was tested for its effect on the progression of the cell cycle, followed by induction of apoptosis determination by flow cytometry. Real-time qRT-PCR was also utilized to observe the initiation of apoptosis. In addition, GC-MS was performed in order to identify the active phytochemicals present in the AHEE. A cytotoxic activity with an IC(50) value of 123.90 μg/mL against HCT-116 colon cancer cells was exhibited by AHEE. Following propidium iodide staining, annexin-V/PI, and clonogenic assays, AHEE treatment results in cell arrest in the S phase, causing an increase in the early and late phases of apoptosis and displaying antiproliferative potential, respectively. The morphological alterations were further monitored using acridine orange/ethidium bromide (AO/EB) staining. When compared with the control cells, features of apoptotic cell death, including nuclear fragmentation, in the AHEE-treated cells were noticed. The apoptosis was also confirmed by detecting the increased expression of p53 and caspase-3 along with the downregulation of Bcl-2. GC-MS analysis revealed that trans-linalool oxide, loliolide, phytol, 4,8,12,16-tetramethylheptadecan-4-olide, and gamma-sitosterol were the major phytochemical constituents. Based on these findings, it can be suggested that AHEE causes cellular death via apoptosis, which should be further explored for the identification of active compounds responsible for these observed effects. Therefore, AHEE can be used in the pharmaceutical development of anticancer agents for cancer therapeutics.
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spelling pubmed-91842052022-06-10 Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling Elasbali, Abdelbaset Mohamed Al-Soud, Waleed Abu Al-Oanzi, Ziad H. Qanash, Husam Alharbi, Bandar Binsaleh, Naif K. Alreshidi, Mousa Patel, Mitesh Adnan, Mohd Evid Based Complement Alternat Med Research Article In the present study, we investigated the cytotoxic effects of Athyrium hohenackerianum ethanolic extract (AHEE) on the proliferation of breast, lung, and colon cancer cells. The AHEE was tested for its effect on the progression of the cell cycle, followed by induction of apoptosis determination by flow cytometry. Real-time qRT-PCR was also utilized to observe the initiation of apoptosis. In addition, GC-MS was performed in order to identify the active phytochemicals present in the AHEE. A cytotoxic activity with an IC(50) value of 123.90 μg/mL against HCT-116 colon cancer cells was exhibited by AHEE. Following propidium iodide staining, annexin-V/PI, and clonogenic assays, AHEE treatment results in cell arrest in the S phase, causing an increase in the early and late phases of apoptosis and displaying antiproliferative potential, respectively. The morphological alterations were further monitored using acridine orange/ethidium bromide (AO/EB) staining. When compared with the control cells, features of apoptotic cell death, including nuclear fragmentation, in the AHEE-treated cells were noticed. The apoptosis was also confirmed by detecting the increased expression of p53 and caspase-3 along with the downregulation of Bcl-2. GC-MS analysis revealed that trans-linalool oxide, loliolide, phytol, 4,8,12,16-tetramethylheptadecan-4-olide, and gamma-sitosterol were the major phytochemical constituents. Based on these findings, it can be suggested that AHEE causes cellular death via apoptosis, which should be further explored for the identification of active compounds responsible for these observed effects. Therefore, AHEE can be used in the pharmaceutical development of anticancer agents for cancer therapeutics. Hindawi 2022-06-02 /pmc/articles/PMC9184205/ /pubmed/35692581 http://dx.doi.org/10.1155/2022/2055773 Text en Copyright © 2022 Abdelbaset Mohamed Elasbali et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Elasbali, Abdelbaset Mohamed
Al-Soud, Waleed Abu
Al-Oanzi, Ziad H.
Qanash, Husam
Alharbi, Bandar
Binsaleh, Naif K.
Alreshidi, Mousa
Patel, Mitesh
Adnan, Mohd
Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title_full Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title_fullStr Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title_full_unstemmed Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title_short Cytotoxic Activity, Cell Cycle Inhibition, and Apoptosis-Inducing Potential of Athyrium hohenackerianum (Lady Fern) with Its Phytochemical Profiling
title_sort cytotoxic activity, cell cycle inhibition, and apoptosis-inducing potential of athyrium hohenackerianum (lady fern) with its phytochemical profiling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184205/
https://www.ncbi.nlm.nih.gov/pubmed/35692581
http://dx.doi.org/10.1155/2022/2055773
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