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Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis

A novel approach involving exogenous oxygen vectors was developed for improving the production of biosynthetic Ansamitocin P-3 (AP-3). Four types of oxygen vectors including soybean oil, n-dodecane, n-hexadecane, and Tween-80 were applied to explore the effect of exogenous oxygen vectors on AP-3 yie...

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Autores principales: Zhu, Xiaolin, Hou, Kaiyao, Zheng, Peiyang, Zhong, Wenya, Guo, Jing, Zhao, Xiyue, Hong, Tingting, Cai, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184225/
https://www.ncbi.nlm.nih.gov/pubmed/35692578
http://dx.doi.org/10.1155/2022/3564185
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author Zhu, Xiaolin
Hou, Kaiyao
Zheng, Peiyang
Zhong, Wenya
Guo, Jing
Zhao, Xiyue
Hong, Tingting
Cai, Zhiqiang
author_facet Zhu, Xiaolin
Hou, Kaiyao
Zheng, Peiyang
Zhong, Wenya
Guo, Jing
Zhao, Xiyue
Hong, Tingting
Cai, Zhiqiang
author_sort Zhu, Xiaolin
collection PubMed
description A novel approach involving exogenous oxygen vectors was developed for improving the production of biosynthetic Ansamitocin P-3 (AP-3). Four types of oxygen vectors including soybean oil, n-dodecane, n-hexadecane, and Tween-80 were applied to explore the effect of exogenous oxygen vectors on AP-3 yield. It was observed that soybean oil exhibited a better ability for promoting AP-3 generation than the other three oxygen vectors. Based on the results of the single-factor experiment, response surface methodology was employed to obtain the optimal soybean oil addition method. The optimum soybean oil concentration was 0.52%, and the addition time was 50 h. Under this condition, the yield of AP-3 reached 106.04 mg/L, which was 49.48% higher than that of the control group without adding oxygen vectors. To further investigate the influence of dissolved oxygen on precious orange tufts actinomycetes variety A. pretiosum strain metabolism and AP-3 yield, metabolomics analysis was carried out by detecting strain intermediate metabolites at various stages under different dissolved oxygen levels. Moreover, differential metabolite screening and metabolic pathway enrichment analysis were combined to exploit the effect mechanism of soybean oil on AP-3 production. Results suggested that primary metabolic levels of the TCA cycle and amino acid metabolism increased with the increase in dissolved oxygen level, which was beneficial to the life activities of bacteria and the synthesis of secondary metabolic precursors, thus increasing the production of AP-3.
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spelling pubmed-91842252022-06-10 Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis Zhu, Xiaolin Hou, Kaiyao Zheng, Peiyang Zhong, Wenya Guo, Jing Zhao, Xiyue Hong, Tingting Cai, Zhiqiang Evid Based Complement Alternat Med Research Article A novel approach involving exogenous oxygen vectors was developed for improving the production of biosynthetic Ansamitocin P-3 (AP-3). Four types of oxygen vectors including soybean oil, n-dodecane, n-hexadecane, and Tween-80 were applied to explore the effect of exogenous oxygen vectors on AP-3 yield. It was observed that soybean oil exhibited a better ability for promoting AP-3 generation than the other three oxygen vectors. Based on the results of the single-factor experiment, response surface methodology was employed to obtain the optimal soybean oil addition method. The optimum soybean oil concentration was 0.52%, and the addition time was 50 h. Under this condition, the yield of AP-3 reached 106.04 mg/L, which was 49.48% higher than that of the control group without adding oxygen vectors. To further investigate the influence of dissolved oxygen on precious orange tufts actinomycetes variety A. pretiosum strain metabolism and AP-3 yield, metabolomics analysis was carried out by detecting strain intermediate metabolites at various stages under different dissolved oxygen levels. Moreover, differential metabolite screening and metabolic pathway enrichment analysis were combined to exploit the effect mechanism of soybean oil on AP-3 production. Results suggested that primary metabolic levels of the TCA cycle and amino acid metabolism increased with the increase in dissolved oxygen level, which was beneficial to the life activities of bacteria and the synthesis of secondary metabolic precursors, thus increasing the production of AP-3. Hindawi 2022-06-02 /pmc/articles/PMC9184225/ /pubmed/35692578 http://dx.doi.org/10.1155/2022/3564185 Text en Copyright © 2022 Xiaolin Zhu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Xiaolin
Hou, Kaiyao
Zheng, Peiyang
Zhong, Wenya
Guo, Jing
Zhao, Xiyue
Hong, Tingting
Cai, Zhiqiang
Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title_full Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title_fullStr Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title_full_unstemmed Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title_short Improvement of Biosynthetic Ansamitocin P-3 Production Based on Oxygen-Vector Screening and Metabonomics Analysis
title_sort improvement of biosynthetic ansamitocin p-3 production based on oxygen-vector screening and metabonomics analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184225/
https://www.ncbi.nlm.nih.gov/pubmed/35692578
http://dx.doi.org/10.1155/2022/3564185
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