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iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings

Drought is a major abiotic stress that harms plant cell physiology and limits the growth and productivity of crops. Maize (Zea mays L.), one of the most drought-susceptible crops, is a major food source for humans and an important resource for industrial bioenergy production; therefore, understandin...

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Autores principales: Ren, Wen, Shi, Zi, Zhou, Miaoyi, Zhao, Bingbing, Li, Hanshuai, Wang, Jiarong, Liu, Ya, Zhao, Jiuran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184573/
https://www.ncbi.nlm.nih.gov/pubmed/35681021
http://dx.doi.org/10.1038/s41598-022-13110-7
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author Ren, Wen
Shi, Zi
Zhou, Miaoyi
Zhao, Bingbing
Li, Hanshuai
Wang, Jiarong
Liu, Ya
Zhao, Jiuran
author_facet Ren, Wen
Shi, Zi
Zhou, Miaoyi
Zhao, Bingbing
Li, Hanshuai
Wang, Jiarong
Liu, Ya
Zhao, Jiuran
author_sort Ren, Wen
collection PubMed
description Drought is a major abiotic stress that harms plant cell physiology and limits the growth and productivity of crops. Maize (Zea mays L.), one of the most drought-susceptible crops, is a major food source for humans and an important resource for industrial bioenergy production; therefore, understanding the mechanisms of the drought response is essential for maize improvement. Using isotopic tagging relative quantitation (iTRAQ)-based protein labeling technology, we detected the proteomic changes in maize leaves under drought stress. Among the 3063 proteins that were identified, the abundance of 214 and 148 proteins increased and decreased, respectively, after three days of drought treatment. These differentially abundant proteins (DAPs) were mainly involved in cell redox homeostasis, cell wall organization, photosynthesis, abscisic acid biosynthesis, and stress-response processes. Furthermore, some of the DAP abundances still differed from the control six days after the drought treatment, most of which were molecular chaperones, heat shock proteins, metabolism-related enzymes, hydrolases, and transmembrane signal receptors. The expression level of some DAPs returned to normal when the water supply was restored, but for others it did not. A significant correlation between the protein and transcript levels was observed following an RT-qPCR analysis. Finally, our research provides insights into the overall mechanism of drought-stress tolerance, and important information for breeding of drought-tolerant maize.
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spelling pubmed-91845732022-06-11 iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings Ren, Wen Shi, Zi Zhou, Miaoyi Zhao, Bingbing Li, Hanshuai Wang, Jiarong Liu, Ya Zhao, Jiuran Sci Rep Article Drought is a major abiotic stress that harms plant cell physiology and limits the growth and productivity of crops. Maize (Zea mays L.), one of the most drought-susceptible crops, is a major food source for humans and an important resource for industrial bioenergy production; therefore, understanding the mechanisms of the drought response is essential for maize improvement. Using isotopic tagging relative quantitation (iTRAQ)-based protein labeling technology, we detected the proteomic changes in maize leaves under drought stress. Among the 3063 proteins that were identified, the abundance of 214 and 148 proteins increased and decreased, respectively, after three days of drought treatment. These differentially abundant proteins (DAPs) were mainly involved in cell redox homeostasis, cell wall organization, photosynthesis, abscisic acid biosynthesis, and stress-response processes. Furthermore, some of the DAP abundances still differed from the control six days after the drought treatment, most of which were molecular chaperones, heat shock proteins, metabolism-related enzymes, hydrolases, and transmembrane signal receptors. The expression level of some DAPs returned to normal when the water supply was restored, but for others it did not. A significant correlation between the protein and transcript levels was observed following an RT-qPCR analysis. Finally, our research provides insights into the overall mechanism of drought-stress tolerance, and important information for breeding of drought-tolerant maize. Nature Publishing Group UK 2022-06-09 /pmc/articles/PMC9184573/ /pubmed/35681021 http://dx.doi.org/10.1038/s41598-022-13110-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ren, Wen
Shi, Zi
Zhou, Miaoyi
Zhao, Bingbing
Li, Hanshuai
Wang, Jiarong
Liu, Ya
Zhao, Jiuran
iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title_full iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title_fullStr iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title_full_unstemmed iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title_short iTRAQ-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
title_sort itraq-based quantitative proteomic analysis provides insight into the drought-stress response in maize seedlings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184573/
https://www.ncbi.nlm.nih.gov/pubmed/35681021
http://dx.doi.org/10.1038/s41598-022-13110-7
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