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Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM

Transmission electron cryo-microscopy (cryo-EM) allows for obtaining 3D structural information by imaging macromolecules embedded in thin layers of amorphous ice. To obtain high-resolution structural information, samples need to be thin to minimize inelastic scattering which blurs images. During dat...

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Autor principal: Hagen, Wim J. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184724/
https://www.ncbi.nlm.nih.gov/pubmed/35693551
http://dx.doi.org/10.3389/fmolb.2022.912363
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author Hagen, Wim J. H.
author_facet Hagen, Wim J. H.
author_sort Hagen, Wim J. H.
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description Transmission electron cryo-microscopy (cryo-EM) allows for obtaining 3D structural information by imaging macromolecules embedded in thin layers of amorphous ice. To obtain high-resolution structural information, samples need to be thin to minimize inelastic scattering which blurs images. During data collection sessions, time spent on finding areas on the cryo-EM grid with optimal ice thickness should be minimized as imaging time on high-end Transmission Electron Microscope TEM systems is costly. Recently, grids covered with thin gold films have become popular due to their stability and reduced beam-induced motion of the sample. Gold foil grids have substantially different densities between the gold foil and ice, effectively resulting in the loss of dynamic range between thin and thick regions of ice, making it challenging to find areas with suitable ice thickness efficiently during grid screening and thus increase expensive imaging time. Here, an energy filter-based plasmon imaging is presented as a fast and easy method for grid screening of the gold foil grids.
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spelling pubmed-91847242022-06-11 Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM Hagen, Wim J. H. Front Mol Biosci Molecular Biosciences Transmission electron cryo-microscopy (cryo-EM) allows for obtaining 3D structural information by imaging macromolecules embedded in thin layers of amorphous ice. To obtain high-resolution structural information, samples need to be thin to minimize inelastic scattering which blurs images. During data collection sessions, time spent on finding areas on the cryo-EM grid with optimal ice thickness should be minimized as imaging time on high-end Transmission Electron Microscope TEM systems is costly. Recently, grids covered with thin gold films have become popular due to their stability and reduced beam-induced motion of the sample. Gold foil grids have substantially different densities between the gold foil and ice, effectively resulting in the loss of dynamic range between thin and thick regions of ice, making it challenging to find areas with suitable ice thickness efficiently during grid screening and thus increase expensive imaging time. Here, an energy filter-based plasmon imaging is presented as a fast and easy method for grid screening of the gold foil grids. Frontiers Media S.A. 2022-05-27 /pmc/articles/PMC9184724/ /pubmed/35693551 http://dx.doi.org/10.3389/fmolb.2022.912363 Text en Copyright © 2022 Hagen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Hagen, Wim J. H.
Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title_full Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title_fullStr Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title_full_unstemmed Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title_short Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
title_sort light ‘em up: efficient screening of gold foil grids in cryo-em
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184724/
https://www.ncbi.nlm.nih.gov/pubmed/35693551
http://dx.doi.org/10.3389/fmolb.2022.912363
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