Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR

Gene functions can be assessed in mouse embryonic stem (ES) cells and in mutant mice derived from mutant ES cells. Here, we describe an approach for efficient isolation of the ES clones carrying deletion mutations at the target genes by CRISPR-Cas9. Two sgRNAs against a target gene are co-expressed...

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Detalles Bibliográficos
Autores principales: Liu, Yuhan, Chen, Qian, Song, Chenglin, Xu, Zhen, Yang, Shuting, Li, Xiajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184805/
https://www.ncbi.nlm.nih.gov/pubmed/35693210
http://dx.doi.org/10.1016/j.xpro.2022.101436

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