Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells

Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a technique to study protein complexes on chromatin. The protocol below describes specific steps for RIME analysis of the male human-derived prostate cancer cell line LNCaP. This approach can also be applied to other prostat...

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Autores principales: Scholtes, Charlotte, Dufour, Catherine R., Giguère, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184806/
https://www.ncbi.nlm.nih.gov/pubmed/35693211
http://dx.doi.org/10.1016/j.xpro.2022.101434
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author Scholtes, Charlotte
Dufour, Catherine R.
Giguère, Vincent
author_facet Scholtes, Charlotte
Dufour, Catherine R.
Giguère, Vincent
author_sort Scholtes, Charlotte
collection PubMed
description Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a technique to study protein complexes on chromatin. The protocol below describes specific steps for RIME analysis of the male human-derived prostate cancer cell line LNCaP. This approach can also be applied to other prostate cancer cell lines such as 22Rv1, DU145, and PC3. For other cell types, we recommend optimizing the number of cell culture plates to ensure adequate sample for mass spectrometry protein detection. For complete details on the use and execution of this protocol, please refer to Mohammed et al. (2016) and Dufour et al. (2022).
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spelling pubmed-91848062022-06-11 Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells Scholtes, Charlotte Dufour, Catherine R. Giguère, Vincent STAR Protoc Protocol Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a technique to study protein complexes on chromatin. The protocol below describes specific steps for RIME analysis of the male human-derived prostate cancer cell line LNCaP. This approach can also be applied to other prostate cancer cell lines such as 22Rv1, DU145, and PC3. For other cell types, we recommend optimizing the number of cell culture plates to ensure adequate sample for mass spectrometry protein detection. For complete details on the use and execution of this protocol, please refer to Mohammed et al. (2016) and Dufour et al. (2022). Elsevier 2022-06-07 /pmc/articles/PMC9184806/ /pubmed/35693211 http://dx.doi.org/10.1016/j.xpro.2022.101434 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Scholtes, Charlotte
Dufour, Catherine R.
Giguère, Vincent
Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title_full Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title_fullStr Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title_full_unstemmed Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title_short Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) to identify chromatin-interactome in prostate cancer cells
title_sort rapid immunoprecipitation mass spectrometry of endogenous protein (rime) to identify chromatin-interactome in prostate cancer cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184806/
https://www.ncbi.nlm.nih.gov/pubmed/35693211
http://dx.doi.org/10.1016/j.xpro.2022.101434
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