Measuring trogocytosis between ovarian tumor and natural killer cells

Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes exi...

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Detalles Bibliográficos
Autores principales: Delgado-Gonzalez, Antonio, Huang, Ying-Wen, Porpiglia, Ermelinda, Donoso, Kenyi, Gonzalez, Veronica D., Fantl, Wendy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185020/
https://www.ncbi.nlm.nih.gov/pubmed/35693208
http://dx.doi.org/10.1016/j.xpro.2022.101425
Descripción
Sumario:Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).

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