Measuring trogocytosis between ovarian tumor and natural killer cells

Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes exi...

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Detalles Bibliográficos
Autores principales: Delgado-Gonzalez, Antonio, Huang, Ying-Wen, Porpiglia, Ermelinda, Donoso, Kenyi, Gonzalez, Veronica D., Fantl, Wendy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185020/
https://www.ncbi.nlm.nih.gov/pubmed/35693208
http://dx.doi.org/10.1016/j.xpro.2022.101425
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author Delgado-Gonzalez, Antonio
Huang, Ying-Wen
Porpiglia, Ermelinda
Donoso, Kenyi
Gonzalez, Veronica D.
Fantl, Wendy J.
author_facet Delgado-Gonzalez, Antonio
Huang, Ying-Wen
Porpiglia, Ermelinda
Donoso, Kenyi
Gonzalez, Veronica D.
Fantl, Wendy J.
author_sort Delgado-Gonzalez, Antonio
collection PubMed
description Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).
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spelling pubmed-91850202022-06-11 Measuring trogocytosis between ovarian tumor and natural killer cells Delgado-Gonzalez, Antonio Huang, Ying-Wen Porpiglia, Ermelinda Donoso, Kenyi Gonzalez, Veronica D. Fantl, Wendy J. STAR Protoc Protocol Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021). Elsevier 2022-06-07 /pmc/articles/PMC9185020/ /pubmed/35693208 http://dx.doi.org/10.1016/j.xpro.2022.101425 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Delgado-Gonzalez, Antonio
Huang, Ying-Wen
Porpiglia, Ermelinda
Donoso, Kenyi
Gonzalez, Veronica D.
Fantl, Wendy J.
Measuring trogocytosis between ovarian tumor and natural killer cells
title Measuring trogocytosis between ovarian tumor and natural killer cells
title_full Measuring trogocytosis between ovarian tumor and natural killer cells
title_fullStr Measuring trogocytosis between ovarian tumor and natural killer cells
title_full_unstemmed Measuring trogocytosis between ovarian tumor and natural killer cells
title_short Measuring trogocytosis between ovarian tumor and natural killer cells
title_sort measuring trogocytosis between ovarian tumor and natural killer cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185020/
https://www.ncbi.nlm.nih.gov/pubmed/35693208
http://dx.doi.org/10.1016/j.xpro.2022.101425
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