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BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma
The aim of the present study was to explore the role and biological function of bromodomain PHD finger transcription factor (BPTF) in T-cell lymphoma. Reverse transcription-quantitative PCR (RT-qPCR), western blotting, immunohistochemistry and bioinformatics analysis were used to determine the expre...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185150/ https://www.ncbi.nlm.nih.gov/pubmed/35720479 http://dx.doi.org/10.3892/ol.2022.13344 |
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author | Bai, Dongyu Zhou, Yong Shen, Fayan Gao, Dehong Suo, Wenhao Zhang, Haiping Li, Heng |
author_facet | Bai, Dongyu Zhou, Yong Shen, Fayan Gao, Dehong Suo, Wenhao Zhang, Haiping Li, Heng |
author_sort | Bai, Dongyu |
collection | PubMed |
description | The aim of the present study was to explore the role and biological function of bromodomain PHD finger transcription factor (BPTF) in T-cell lymphoma. Reverse transcription-quantitative PCR (RT-qPCR), western blotting, immunohistochemistry and bioinformatics analysis were used to determine the expression levels of BPTF and Raf1 in T-cell lymphoma tissues and matched adjacent normal tissues. RT-qPCR and western blot analyses were used to examine the role of BPTF in the activation of MAPK signaling. The function of BPTF and Raf1 in T-cell lymphoma was investigated through in vitro and in vivo assays (MTT assay, colony formation assay, flow cytometry, western blotting, tumor xenograft model and TUNEL assay) following silencing and overexpression experiments in Hut-102 cells. The results demonstrated that BPTF and Raf1 were overexpressed in T-cell lymphoma tissues compared with normal tissues, and high expression of BPTF or Raf1 was associated with advanced clinical stage. BPTF promoted the activation of the MAPK pathway and was coexpressed with Raf1 in T-cell lymphoma tissues. Functional assays demonstrated that silencing of BPTF or Raf1 in Hut-102 cells suppressed cell proliferation and induced apoptosis. Furthermore, the carcinogenic effect of BPTF was confirmed by xenograft experiments in nude mice. The present findings suggested that BPTF may function as a crucial oncogenic factor and may serve as a novel therapeutic target in T-cell lymphoma. |
format | Online Article Text |
id | pubmed-9185150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-91851502022-06-16 BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma Bai, Dongyu Zhou, Yong Shen, Fayan Gao, Dehong Suo, Wenhao Zhang, Haiping Li, Heng Oncol Lett Articles The aim of the present study was to explore the role and biological function of bromodomain PHD finger transcription factor (BPTF) in T-cell lymphoma. Reverse transcription-quantitative PCR (RT-qPCR), western blotting, immunohistochemistry and bioinformatics analysis were used to determine the expression levels of BPTF and Raf1 in T-cell lymphoma tissues and matched adjacent normal tissues. RT-qPCR and western blot analyses were used to examine the role of BPTF in the activation of MAPK signaling. The function of BPTF and Raf1 in T-cell lymphoma was investigated through in vitro and in vivo assays (MTT assay, colony formation assay, flow cytometry, western blotting, tumor xenograft model and TUNEL assay) following silencing and overexpression experiments in Hut-102 cells. The results demonstrated that BPTF and Raf1 were overexpressed in T-cell lymphoma tissues compared with normal tissues, and high expression of BPTF or Raf1 was associated with advanced clinical stage. BPTF promoted the activation of the MAPK pathway and was coexpressed with Raf1 in T-cell lymphoma tissues. Functional assays demonstrated that silencing of BPTF or Raf1 in Hut-102 cells suppressed cell proliferation and induced apoptosis. Furthermore, the carcinogenic effect of BPTF was confirmed by xenograft experiments in nude mice. The present findings suggested that BPTF may function as a crucial oncogenic factor and may serve as a novel therapeutic target in T-cell lymphoma. D.A. Spandidos 2022-05-25 /pmc/articles/PMC9185150/ /pubmed/35720479 http://dx.doi.org/10.3892/ol.2022.13344 Text en Copyright: © Bai et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Bai, Dongyu Zhou, Yong Shen, Fayan Gao, Dehong Suo, Wenhao Zhang, Haiping Li, Heng BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title | BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title_full | BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title_fullStr | BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title_full_unstemmed | BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title_short | BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma |
title_sort | bptf activates the mapk pathway through coexpression with raf1 to promote proliferation of t-cell lymphoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185150/ https://www.ncbi.nlm.nih.gov/pubmed/35720479 http://dx.doi.org/10.3892/ol.2022.13344 |
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