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Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest

[Image: see text] Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this p...

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Autores principales: Deroy, Cyril, Wheeler, James H. R., Rumianek, Agata N., Cook, Peter R., Durham, William M., Foster, Kevin R., Walsh, Edmond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185686/
https://www.ncbi.nlm.nih.gov/pubmed/35604799
http://dx.doi.org/10.1021/acsami.2c07177
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author Deroy, Cyril
Wheeler, James H. R.
Rumianek, Agata N.
Cook, Peter R.
Durham, William M.
Foster, Kevin R.
Walsh, Edmond J.
author_facet Deroy, Cyril
Wheeler, James H. R.
Rumianek, Agata N.
Cook, Peter R.
Durham, William M.
Foster, Kevin R.
Walsh, Edmond J.
author_sort Deroy, Cyril
collection PubMed
description [Image: see text] Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this problem that exploits the remarkable properties of microfluidic circuits with fluid walls—transparent interfaces between culture media and an immiscible fluorocarbon that are easily pierced with pipets. We provide two proofs of concept in which specific cell subpopulations are isolated and recovered: (i) murine macrophages chemotaxing toward complement component 5a and (ii) bacteria (Pseudomonas aeruginosa) in developing biofilms that migrate toward antibiotics. We build circuits in minutes on standard Petri dishes, add cells, pump in laminar streams so molecular diffusion creates attractant gradients, acquire time-lapse images, and isolate desired subpopulations in real time by building fluid walls around migrating cells with an accuracy of tens of micrometers using 3D printed adaptors that convert conventional microscopes into wall-building machines. Our method allows live cells of interest to be easily extracted from microfluidic devices for downstream analyses.
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spelling pubmed-91856862022-06-11 Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest Deroy, Cyril Wheeler, James H. R. Rumianek, Agata N. Cook, Peter R. Durham, William M. Foster, Kevin R. Walsh, Edmond J. ACS Appl Mater Interfaces [Image: see text] Microfluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this problem that exploits the remarkable properties of microfluidic circuits with fluid walls—transparent interfaces between culture media and an immiscible fluorocarbon that are easily pierced with pipets. We provide two proofs of concept in which specific cell subpopulations are isolated and recovered: (i) murine macrophages chemotaxing toward complement component 5a and (ii) bacteria (Pseudomonas aeruginosa) in developing biofilms that migrate toward antibiotics. We build circuits in minutes on standard Petri dishes, add cells, pump in laminar streams so molecular diffusion creates attractant gradients, acquire time-lapse images, and isolate desired subpopulations in real time by building fluid walls around migrating cells with an accuracy of tens of micrometers using 3D printed adaptors that convert conventional microscopes into wall-building machines. Our method allows live cells of interest to be easily extracted from microfluidic devices for downstream analyses. American Chemical Society 2022-05-23 2022-06-08 /pmc/articles/PMC9185686/ /pubmed/35604799 http://dx.doi.org/10.1021/acsami.2c07177 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Deroy, Cyril
Wheeler, James H. R.
Rumianek, Agata N.
Cook, Peter R.
Durham, William M.
Foster, Kevin R.
Walsh, Edmond J.
Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title_full Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title_fullStr Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title_full_unstemmed Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title_short Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest
title_sort reconfigurable microfluidic circuits for isolating and retrieving cells of interest
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185686/
https://www.ncbi.nlm.nih.gov/pubmed/35604799
http://dx.doi.org/10.1021/acsami.2c07177
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