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CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone

Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies and primarily presents with hyperandrogenism. Although environmental factors and genetic factors are thought to be the major reason, there still exists a lot of questions need to be answered. High expression of C-terminal-bi...

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Autores principales: Wen, Mingxiao, Dou, Xiaoqing, Zhang, Shuzhen, Wang, Bin, Xu, Jiangyan, Zhang, Wenchao, Wang, Feifei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185693/
https://www.ncbi.nlm.nih.gov/pubmed/35656892
http://dx.doi.org/10.3892/mmr.2022.12761
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author Wen, Mingxiao
Dou, Xiaoqing
Zhang, Shuzhen
Wang, Bin
Xu, Jiangyan
Zhang, Wenchao
Wang, Feifei
author_facet Wen, Mingxiao
Dou, Xiaoqing
Zhang, Shuzhen
Wang, Bin
Xu, Jiangyan
Zhang, Wenchao
Wang, Feifei
author_sort Wen, Mingxiao
collection PubMed
description Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies and primarily presents with hyperandrogenism. Although environmental factors and genetic factors are thought to be the major reason, there still exists a lot of questions need to be answered. High expression of C-terminal-binding protein 1 antisense (CTBP1-AS) was identified as an independent risk factor for PCOS; however, the molecular mechanism of CTBP1-AS in PCOS regulation is unknown. In the present study, the expression level of CTBP1-AS was found to be significantly upregulated in patients with PCOS compared with healthy control patients. CTBP1-AS knockdown was demonstrated to reduce the proliferation and promote the apoptosis of granulosa tumor cells in vitro. It was also identified that the two core catalytic subunits of Polycomb repressive complex 2 (enhancer of zeste homolog 2 and embryonic and ectoderm development protein) interacted with CTBP1-AS in primary granulosa cells and KGN cells. In addition, cryptotanshinone treatment was demonstrated to effectively downregulate CTBP1-AS expression level. Data from the present study suggested a pathophysiological role of CTBP1-AS in PCOS and may provide a new potential target for PCOS treatment.
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spelling pubmed-91856932022-06-16 CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone Wen, Mingxiao Dou, Xiaoqing Zhang, Shuzhen Wang, Bin Xu, Jiangyan Zhang, Wenchao Wang, Feifei Mol Med Rep Articles Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies and primarily presents with hyperandrogenism. Although environmental factors and genetic factors are thought to be the major reason, there still exists a lot of questions need to be answered. High expression of C-terminal-binding protein 1 antisense (CTBP1-AS) was identified as an independent risk factor for PCOS; however, the molecular mechanism of CTBP1-AS in PCOS regulation is unknown. In the present study, the expression level of CTBP1-AS was found to be significantly upregulated in patients with PCOS compared with healthy control patients. CTBP1-AS knockdown was demonstrated to reduce the proliferation and promote the apoptosis of granulosa tumor cells in vitro. It was also identified that the two core catalytic subunits of Polycomb repressive complex 2 (enhancer of zeste homolog 2 and embryonic and ectoderm development protein) interacted with CTBP1-AS in primary granulosa cells and KGN cells. In addition, cryptotanshinone treatment was demonstrated to effectively downregulate CTBP1-AS expression level. Data from the present study suggested a pathophysiological role of CTBP1-AS in PCOS and may provide a new potential target for PCOS treatment. D.A. Spandidos 2022-06-01 /pmc/articles/PMC9185693/ /pubmed/35656892 http://dx.doi.org/10.3892/mmr.2022.12761 Text en Copyright: © Wen et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wen, Mingxiao
Dou, Xiaoqing
Zhang, Shuzhen
Wang, Bin
Xu, Jiangyan
Zhang, Wenchao
Wang, Feifei
CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title_full CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title_fullStr CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title_full_unstemmed CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title_short CTBP1-AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
title_sort ctbp1-as upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185693/
https://www.ncbi.nlm.nih.gov/pubmed/35656892
http://dx.doi.org/10.3892/mmr.2022.12761
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