Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction

Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1–1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa,...

Descripción completa

Detalles Bibliográficos
Autores principales: Oshiro, Satoshi, Mizutani, Naeko, Tada, Tatsuya, Sekiguchi, Jun-Ichiro, Takahashi, Masao, Kirikae, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186408/
https://www.ncbi.nlm.nih.gov/pubmed/35702275
http://dx.doi.org/10.1016/j.idairyj.2022.105436
_version_ 1784724925791076352
author Oshiro, Satoshi
Mizutani, Naeko
Tada, Tatsuya
Sekiguchi, Jun-Ichiro
Takahashi, Masao
Kirikae, Teruo
author_facet Oshiro, Satoshi
Mizutani, Naeko
Tada, Tatsuya
Sekiguchi, Jun-Ichiro
Takahashi, Masao
Kirikae, Teruo
author_sort Oshiro, Satoshi
collection PubMed
description Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1–1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa, and omicron, which contain 10, eight, seven, and over 30 mutations in the spike protein, respectively. We investigated whether bovine whey IgG enriched fraction contains antibodies against spike proteins of these variants, specifically recombinant partial length spike proteins (aa 177–512, aa 509–685, aa 177–324, aa 250–410 and aa 387–516) of these variants. Direct enzyme-linked immunosorbent assays revealed bovine whey IgG enriched fraction contained antibodies against all recombinant spike proteins of these variants with highest reactivity against aa 177–512 region of omicron spike protein. These results indicate bovine whey IgG enriched fraction contains antibodies against spike proteins of several SARS-CoV-2 variants, including omicron.
format Online
Article
Text
id pubmed-9186408
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-91864082022-06-10 Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction Oshiro, Satoshi Mizutani, Naeko Tada, Tatsuya Sekiguchi, Jun-Ichiro Takahashi, Masao Kirikae, Teruo Int Dairy J Short Communication Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1–1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa, and omicron, which contain 10, eight, seven, and over 30 mutations in the spike protein, respectively. We investigated whether bovine whey IgG enriched fraction contains antibodies against spike proteins of these variants, specifically recombinant partial length spike proteins (aa 177–512, aa 509–685, aa 177–324, aa 250–410 and aa 387–516) of these variants. Direct enzyme-linked immunosorbent assays revealed bovine whey IgG enriched fraction contained antibodies against all recombinant spike proteins of these variants with highest reactivity against aa 177–512 region of omicron spike protein. These results indicate bovine whey IgG enriched fraction contains antibodies against spike proteins of several SARS-CoV-2 variants, including omicron. Elsevier Ltd. 2022-10 2022-06-10 /pmc/articles/PMC9186408/ /pubmed/35702275 http://dx.doi.org/10.1016/j.idairyj.2022.105436 Text en © 2022 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Oshiro, Satoshi
Mizutani, Naeko
Tada, Tatsuya
Sekiguchi, Jun-Ichiro
Takahashi, Masao
Kirikae, Teruo
Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title_full Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title_fullStr Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title_full_unstemmed Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title_short Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction
title_sort antibodies against spike protein of sars-cov-2 variants in bovine whey igg enriched fraction
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186408/
https://www.ncbi.nlm.nih.gov/pubmed/35702275
http://dx.doi.org/10.1016/j.idairyj.2022.105436
work_keys_str_mv AT oshirosatoshi antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction
AT mizutaninaeko antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction
AT tadatatsuya antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction
AT sekiguchijunichiro antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction
AT takahashimasao antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction
AT kirikaeteruo antibodiesagainstspikeproteinofsarscov2variantsinbovinewheyiggenrichedfraction

Ejemplares similares