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miR-30 inhibits the progression of osteosarcoma by targeting MTA1
OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Society of Musculoskeletal and Neuronal Interactions
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186454/ https://www.ncbi.nlm.nih.gov/pubmed/35642705 |
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author | Zhao, Aiqing Zhao, Yanbin Feng, Wei Zhao, Zhenqun Liu, Wanlin Wang, Na Xue, Huiqin Wu, Lishuan Cui, Shuxia Bai, Rui |
author_facet | Zhao, Aiqing Zhao, Yanbin Feng, Wei Zhao, Zhenqun Liu, Wanlin Wang, Na Xue, Huiqin Wu, Lishuan Cui, Shuxia Bai, Rui |
author_sort | Zhao, Aiqing |
collection | PubMed |
description | OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used to detect mRNA levels of miR-30 and MTA1 in cancer tissues, adjacent non-cancerous tissues from OS patients. Western blot was used to detect MTA1 protein expression in all tissues and cell lines (hFOb1.19,Saos-2, MG63, and U2OS). The correlation between miR-30 and MTA1 was predicted through bioinformatics software, and identified by a luciferase reporting experiment. In vitro, functional test detected the specific effects of miR-30 and MTA1 on the development of OS. RESULTS: miR-30 expression was significantly reduced, while the expression of MTA1 was increased in OS tissues and cells. Luciferase reporting experiment showed that miR-30 sponged MTA1 which was negatively correlated with miR-30 expression. Furthermore, rescue tests revealed that MTA1 restrained the functions of miR-30 on cell proliferation and migration of OS. CONCLUSION: Our finding showed that miR-30 modulated the proliferation and migration by targeting MTA1 in OS. |
format | Online Article Text |
id | pubmed-9186454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Society of Musculoskeletal and Neuronal Interactions |
record_format | MEDLINE/PubMed |
spelling | pubmed-91864542022-07-05 miR-30 inhibits the progression of osteosarcoma by targeting MTA1 Zhao, Aiqing Zhao, Yanbin Feng, Wei Zhao, Zhenqun Liu, Wanlin Wang, Na Xue, Huiqin Wu, Lishuan Cui, Shuxia Bai, Rui J Musculoskelet Neuronal Interact Original Article OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used to detect mRNA levels of miR-30 and MTA1 in cancer tissues, adjacent non-cancerous tissues from OS patients. Western blot was used to detect MTA1 protein expression in all tissues and cell lines (hFOb1.19,Saos-2, MG63, and U2OS). The correlation between miR-30 and MTA1 was predicted through bioinformatics software, and identified by a luciferase reporting experiment. In vitro, functional test detected the specific effects of miR-30 and MTA1 on the development of OS. RESULTS: miR-30 expression was significantly reduced, while the expression of MTA1 was increased in OS tissues and cells. Luciferase reporting experiment showed that miR-30 sponged MTA1 which was negatively correlated with miR-30 expression. Furthermore, rescue tests revealed that MTA1 restrained the functions of miR-30 on cell proliferation and migration of OS. CONCLUSION: Our finding showed that miR-30 modulated the proliferation and migration by targeting MTA1 in OS. International Society of Musculoskeletal and Neuronal Interactions 2022 /pmc/articles/PMC9186454/ /pubmed/35642705 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zhao, Aiqing Zhao, Yanbin Feng, Wei Zhao, Zhenqun Liu, Wanlin Wang, Na Xue, Huiqin Wu, Lishuan Cui, Shuxia Bai, Rui miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title | miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title_full | miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title_fullStr | miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title_full_unstemmed | miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title_short | miR-30 inhibits the progression of osteosarcoma by targeting MTA1 |
title_sort | mir-30 inhibits the progression of osteosarcoma by targeting mta1 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186454/ https://www.ncbi.nlm.nih.gov/pubmed/35642705 |
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