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miR-30 inhibits the progression of osteosarcoma by targeting MTA1

OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used...

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Autores principales: Zhao, Aiqing, Zhao, Yanbin, Feng, Wei, Zhao, Zhenqun, Liu, Wanlin, Wang, Na, Xue, Huiqin, Wu, Lishuan, Cui, Shuxia, Bai, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society of Musculoskeletal and Neuronal Interactions 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186454/
https://www.ncbi.nlm.nih.gov/pubmed/35642705
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author Zhao, Aiqing
Zhao, Yanbin
Feng, Wei
Zhao, Zhenqun
Liu, Wanlin
Wang, Na
Xue, Huiqin
Wu, Lishuan
Cui, Shuxia
Bai, Rui
author_facet Zhao, Aiqing
Zhao, Yanbin
Feng, Wei
Zhao, Zhenqun
Liu, Wanlin
Wang, Na
Xue, Huiqin
Wu, Lishuan
Cui, Shuxia
Bai, Rui
author_sort Zhao, Aiqing
collection PubMed
description OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used to detect mRNA levels of miR-30 and MTA1 in cancer tissues, adjacent non-cancerous tissues from OS patients. Western blot was used to detect MTA1 protein expression in all tissues and cell lines (hFOb1.19,Saos-2, MG63, and U2OS). The correlation between miR-30 and MTA1 was predicted through bioinformatics software, and identified by a luciferase reporting experiment. In vitro, functional test detected the specific effects of miR-30 and MTA1 on the development of OS. RESULTS: miR-30 expression was significantly reduced, while the expression of MTA1 was increased in OS tissues and cells. Luciferase reporting experiment showed that miR-30 sponged MTA1 which was negatively correlated with miR-30 expression. Furthermore, rescue tests revealed that MTA1 restrained the functions of miR-30 on cell proliferation and migration of OS. CONCLUSION: Our finding showed that miR-30 modulated the proliferation and migration by targeting MTA1 in OS.
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spelling pubmed-91864542022-07-05 miR-30 inhibits the progression of osteosarcoma by targeting MTA1 Zhao, Aiqing Zhao, Yanbin Feng, Wei Zhao, Zhenqun Liu, Wanlin Wang, Na Xue, Huiqin Wu, Lishuan Cui, Shuxia Bai, Rui J Musculoskelet Neuronal Interact Original Article OBJECTIVES: MicroRNAs (miRNAs) have been considered as a new class of novel diagnostic and predictive biomarker in many diseases. However, there are few studies on miRNA in osteosarcoma (OS). This study aimed to investigate the roles of miR-30 on OS occurrence and development. METHODS: PCR was used to detect mRNA levels of miR-30 and MTA1 in cancer tissues, adjacent non-cancerous tissues from OS patients. Western blot was used to detect MTA1 protein expression in all tissues and cell lines (hFOb1.19,Saos-2, MG63, and U2OS). The correlation between miR-30 and MTA1 was predicted through bioinformatics software, and identified by a luciferase reporting experiment. In vitro, functional test detected the specific effects of miR-30 and MTA1 on the development of OS. RESULTS: miR-30 expression was significantly reduced, while the expression of MTA1 was increased in OS tissues and cells. Luciferase reporting experiment showed that miR-30 sponged MTA1 which was negatively correlated with miR-30 expression. Furthermore, rescue tests revealed that MTA1 restrained the functions of miR-30 on cell proliferation and migration of OS. CONCLUSION: Our finding showed that miR-30 modulated the proliferation and migration by targeting MTA1 in OS. International Society of Musculoskeletal and Neuronal Interactions 2022 /pmc/articles/PMC9186454/ /pubmed/35642705 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhao, Aiqing
Zhao, Yanbin
Feng, Wei
Zhao, Zhenqun
Liu, Wanlin
Wang, Na
Xue, Huiqin
Wu, Lishuan
Cui, Shuxia
Bai, Rui
miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title_full miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title_fullStr miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title_full_unstemmed miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title_short miR-30 inhibits the progression of osteosarcoma by targeting MTA1
title_sort mir-30 inhibits the progression of osteosarcoma by targeting mta1
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186454/
https://www.ncbi.nlm.nih.gov/pubmed/35642705
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