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Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity

Telomerase activity is essential for the self-renewal and potential of embryonic, induced pluripotent, and cancer stem cells, as well as a few somatic stem cells, such as human urine-derived stem cells (USCs). However, it remains unclear how telomerase activity affects the regeneration potential of...

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Autores principales: Shi, Yingai, Liu, Guihua, Wu, Rongpei, Mack, David L., Sun, Xiuzhi Susan, Maxwell, Joshua, Guan, Xuan, Atala, Anthony, Zhang, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186504/
https://www.ncbi.nlm.nih.gov/pubmed/35693947
http://dx.doi.org/10.3389/fcell.2022.890574
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author Shi, Yingai
Liu, Guihua
Wu, Rongpei
Mack, David L.
Sun, Xiuzhi Susan
Maxwell, Joshua
Guan, Xuan
Atala, Anthony
Zhang, Yuanyuan
author_facet Shi, Yingai
Liu, Guihua
Wu, Rongpei
Mack, David L.
Sun, Xiuzhi Susan
Maxwell, Joshua
Guan, Xuan
Atala, Anthony
Zhang, Yuanyuan
author_sort Shi, Yingai
collection PubMed
description Telomerase activity is essential for the self-renewal and potential of embryonic, induced pluripotent, and cancer stem cells, as well as a few somatic stem cells, such as human urine-derived stem cells (USCs). However, it remains unclear how telomerase activity affects the regeneration potential of somatic stem cells. The objective of this study was to determine the regenerative significance of telomerase activity, particularly to retain cell surface marker expression, multipotent differentiation capability, chromosomal stability, and in vivo tumorigenic transformation, in each clonal population of human primary USCs. In total, 117 USC specimens from 10 healthy male adults (25–57 years of age) were obtained. Polymerase chain reaction amplification of a telomeric repeat was used to detect USCs with positive telomerase activity (USCs(TA+)). A total of 80 USCs(TA+) (70.2%) were identified from 117 USC clones, but they were not detected in the paired normal bladder smooth muscle cell and bone marrow stromal cell specimens. In the 20–40 years age group, approximately 75% of USC clones displayed positive telomerase activity, whereas in the 50 years age group, 59.2% of the USC clones expressed positive telomerase activity. USCs(TA+) extended to passage 16, underwent 62.0 ± 4.8 population doublings, produced more cells, and were superior for osteogenic, myogenic, and uroepithelial differentiation compared to USCs(TA−). Importantly, USCs displayed normal chromosome and no oncological transformation after being implanted in vivo. Overall, as a safe cell source, telomerase-positive USCs have a robust regenerative potential in cell proliferation and multipotent differentiation capacity.
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spelling pubmed-91865042022-06-11 Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity Shi, Yingai Liu, Guihua Wu, Rongpei Mack, David L. Sun, Xiuzhi Susan Maxwell, Joshua Guan, Xuan Atala, Anthony Zhang, Yuanyuan Front Cell Dev Biol Cell and Developmental Biology Telomerase activity is essential for the self-renewal and potential of embryonic, induced pluripotent, and cancer stem cells, as well as a few somatic stem cells, such as human urine-derived stem cells (USCs). However, it remains unclear how telomerase activity affects the regeneration potential of somatic stem cells. The objective of this study was to determine the regenerative significance of telomerase activity, particularly to retain cell surface marker expression, multipotent differentiation capability, chromosomal stability, and in vivo tumorigenic transformation, in each clonal population of human primary USCs. In total, 117 USC specimens from 10 healthy male adults (25–57 years of age) were obtained. Polymerase chain reaction amplification of a telomeric repeat was used to detect USCs with positive telomerase activity (USCs(TA+)). A total of 80 USCs(TA+) (70.2%) were identified from 117 USC clones, but they were not detected in the paired normal bladder smooth muscle cell and bone marrow stromal cell specimens. In the 20–40 years age group, approximately 75% of USC clones displayed positive telomerase activity, whereas in the 50 years age group, 59.2% of the USC clones expressed positive telomerase activity. USCs(TA+) extended to passage 16, underwent 62.0 ± 4.8 population doublings, produced more cells, and were superior for osteogenic, myogenic, and uroepithelial differentiation compared to USCs(TA−). Importantly, USCs displayed normal chromosome and no oncological transformation after being implanted in vivo. Overall, as a safe cell source, telomerase-positive USCs have a robust regenerative potential in cell proliferation and multipotent differentiation capacity. Frontiers Media S.A. 2022-05-23 /pmc/articles/PMC9186504/ /pubmed/35693947 http://dx.doi.org/10.3389/fcell.2022.890574 Text en Copyright © 2022 Shi, Liu, Wu, Mack, Sun, Maxwell, Guan, Atala and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Shi, Yingai
Liu, Guihua
Wu, Rongpei
Mack, David L.
Sun, Xiuzhi Susan
Maxwell, Joshua
Guan, Xuan
Atala, Anthony
Zhang, Yuanyuan
Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title_full Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title_fullStr Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title_full_unstemmed Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title_short Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity
title_sort differentiation capacity of human urine-derived stem cells to retain telomerase activity
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186504/
https://www.ncbi.nlm.nih.gov/pubmed/35693947
http://dx.doi.org/10.3389/fcell.2022.890574
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