Cargando…

Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is the most frequently encountered type of oral cancer. Histamine receptor H1 (HRH1) was reported to play a crucial role in OSCC carcinogenesis, but impacts of genetic variants of HRH1 on OSCC remain unclear. Herein, we investigated the association between functio...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Yi-Fang, Lin, Yung-Wei, Chiu, Wen-Kuan, Lin, Chiao-Wen, Yang, Yi-Chieh, Chang, Lun-Ching, Chang, Jungshan, Yang, Shun-Fa, Chien, Ming-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186772/
https://www.ncbi.nlm.nih.gov/pubmed/35587368
http://dx.doi.org/10.18632/aging.204089
_version_ 1784725018319519744
author Ding, Yi-Fang
Lin, Yung-Wei
Chiu, Wen-Kuan
Lin, Chiao-Wen
Yang, Yi-Chieh
Chang, Lun-Ching
Chang, Jungshan
Yang, Shun-Fa
Chien, Ming-Hsien
author_facet Ding, Yi-Fang
Lin, Yung-Wei
Chiu, Wen-Kuan
Lin, Chiao-Wen
Yang, Yi-Chieh
Chang, Lun-Ching
Chang, Jungshan
Yang, Shun-Fa
Chien, Ming-Hsien
author_sort Ding, Yi-Fang
collection PubMed
description Oral squamous cell carcinoma (OSCC) is the most frequently encountered type of oral cancer. Histamine receptor H1 (HRH1) was reported to play a crucial role in OSCC carcinogenesis, but impacts of genetic variants of HRH1 on OSCC remain unclear. Herein, we investigated the association between functional single-nucleotide polymorphisms (SNPs) of HRH1 and OSCC susceptibility or clinicopathologic variables by logistic regression models. HRH1 genotypes at four loci (rs346074, rs346076, rs901865, and rs2606731) were analyzed by a TaqMan allelic discrimination assay, and we found that patients harboring HRH1 rs901865 T and rs346074 T alleles had a significantly lower risk of developing larger tumor sizes (>T2) under a dominant model. Based on the environmental carcinogen exposure status, we observed that HRH1 rs901865 polymorphic variants were also associated with a lower risk of developing more-advanced clinical stages (III or IV) in patients with a betel-quid-chewing habit. Moreover, genotype screening of rs901865 and rs346074 in OSCC cell lines showed that cells respectively carrying the CT and TT genotypes expressed lower HRH1 levels compared to cells carrying the CC genotype of rs901865 and rs346074. Furthermore, analyses of TCGA and GEO databases revealed that HRH1 expression levels were upregulated in head and neck squamous cell carcinoma (HNSCC) and OSCC tissues compared to normal tissues and were correlated with larger tumor sizes and poorer prognoses. These results indicated the involvement of HRH1 SNPs rs901865 and rs346074 in OSCC development and support the interaction between HRH1 gene polymorphisms and an environmental carcinogen as a predisposing factor for OSCC progression.
format Online
Article
Text
id pubmed-9186772
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-91867722022-06-14 Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma Ding, Yi-Fang Lin, Yung-Wei Chiu, Wen-Kuan Lin, Chiao-Wen Yang, Yi-Chieh Chang, Lun-Ching Chang, Jungshan Yang, Shun-Fa Chien, Ming-Hsien Aging (Albany NY) Research Paper Oral squamous cell carcinoma (OSCC) is the most frequently encountered type of oral cancer. Histamine receptor H1 (HRH1) was reported to play a crucial role in OSCC carcinogenesis, but impacts of genetic variants of HRH1 on OSCC remain unclear. Herein, we investigated the association between functional single-nucleotide polymorphisms (SNPs) of HRH1 and OSCC susceptibility or clinicopathologic variables by logistic regression models. HRH1 genotypes at four loci (rs346074, rs346076, rs901865, and rs2606731) were analyzed by a TaqMan allelic discrimination assay, and we found that patients harboring HRH1 rs901865 T and rs346074 T alleles had a significantly lower risk of developing larger tumor sizes (>T2) under a dominant model. Based on the environmental carcinogen exposure status, we observed that HRH1 rs901865 polymorphic variants were also associated with a lower risk of developing more-advanced clinical stages (III or IV) in patients with a betel-quid-chewing habit. Moreover, genotype screening of rs901865 and rs346074 in OSCC cell lines showed that cells respectively carrying the CT and TT genotypes expressed lower HRH1 levels compared to cells carrying the CC genotype of rs901865 and rs346074. Furthermore, analyses of TCGA and GEO databases revealed that HRH1 expression levels were upregulated in head and neck squamous cell carcinoma (HNSCC) and OSCC tissues compared to normal tissues and were correlated with larger tumor sizes and poorer prognoses. These results indicated the involvement of HRH1 SNPs rs901865 and rs346074 in OSCC development and support the interaction between HRH1 gene polymorphisms and an environmental carcinogen as a predisposing factor for OSCC progression. Impact Journals 2022-05-19 /pmc/articles/PMC9186772/ /pubmed/35587368 http://dx.doi.org/10.18632/aging.204089 Text en Copyright: © 2022 Ding et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ding, Yi-Fang
Lin, Yung-Wei
Chiu, Wen-Kuan
Lin, Chiao-Wen
Yang, Yi-Chieh
Chang, Lun-Ching
Chang, Jungshan
Yang, Shun-Fa
Chien, Ming-Hsien
Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title_full Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title_fullStr Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title_full_unstemmed Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title_short Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
title_sort combined impacts of histamine receptor h1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186772/
https://www.ncbi.nlm.nih.gov/pubmed/35587368
http://dx.doi.org/10.18632/aging.204089
work_keys_str_mv AT dingyifang combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT linyungwei combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT chiuwenkuan combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT linchiaowen combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT yangyichieh combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT changlunching combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT changjungshan combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT yangshunfa combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma
AT chienminghsien combinedimpactsofhistaminereceptorh1genepolymorphismsandanenvironmentalcarcinogenonthesusceptibilitytoandprogressionoforalsquamouscellcarcinoma