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Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to impose a serious burden on health systems globally. Despite worldwide vaccination, social distancing and wearing masks, the spread of the virus is ongoing. One of the mechanisms by which neutralizing antibodies (NAbs) block vi...

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Autores principales: Kangro, Kadri, Kurašin, Mihhail, Gildemann, Kiira, Sankovski, Eve, Žusinaite, Eva, Lello, Laura Sandra, Pert, Raini, Kavak, Ants, Poikalainen, Väino, Lepasalu, Lembit, Kuusk, Marilin, Pau, Robin, Piiskop, Sander, Rom, Siimu, Oltjer, Ruth, Tiirik, Kairi, Kogermann, Karin, Plaas, Mario, Tiirats, Toomas, Aasmäe, Birgit, Plaas, Mihkel, Mumm, Karl, Krinka, Dagni, Talpsep, Ene, Kadaja, Meelis, Gerhold, Joachim M., Planken, Anu, Tover, Andres, Merits, Andres, Männik, Andres, Ustav, Mart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187060/
https://www.ncbi.nlm.nih.gov/pubmed/35687549
http://dx.doi.org/10.1371/journal.pone.0268806
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author Kangro, Kadri
Kurašin, Mihhail
Gildemann, Kiira
Sankovski, Eve
Žusinaite, Eva
Lello, Laura Sandra
Pert, Raini
Kavak, Ants
Poikalainen, Väino
Lepasalu, Lembit
Kuusk, Marilin
Pau, Robin
Piiskop, Sander
Rom, Siimu
Oltjer, Ruth
Tiirik, Kairi
Kogermann, Karin
Plaas, Mario
Tiirats, Toomas
Aasmäe, Birgit
Plaas, Mihkel
Mumm, Karl
Krinka, Dagni
Talpsep, Ene
Kadaja, Meelis
Gerhold, Joachim M.
Planken, Anu
Tover, Andres
Merits, Andres
Männik, Andres
Ustav, Mart
Ustav, Mart
author_facet Kangro, Kadri
Kurašin, Mihhail
Gildemann, Kiira
Sankovski, Eve
Žusinaite, Eva
Lello, Laura Sandra
Pert, Raini
Kavak, Ants
Poikalainen, Väino
Lepasalu, Lembit
Kuusk, Marilin
Pau, Robin
Piiskop, Sander
Rom, Siimu
Oltjer, Ruth
Tiirik, Kairi
Kogermann, Karin
Plaas, Mario
Tiirats, Toomas
Aasmäe, Birgit
Plaas, Mihkel
Mumm, Karl
Krinka, Dagni
Talpsep, Ene
Kadaja, Meelis
Gerhold, Joachim M.
Planken, Anu
Tover, Andres
Merits, Andres
Männik, Andres
Ustav, Mart
Ustav, Mart
author_sort Kangro, Kadri
collection PubMed
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to impose a serious burden on health systems globally. Despite worldwide vaccination, social distancing and wearing masks, the spread of the virus is ongoing. One of the mechanisms by which neutralizing antibodies (NAbs) block virus entry into cells encompasses interaction inhibition between the cell surface receptor angiotensin-converting enzyme 2 (ACE2) and the spike (S) protein of SARS-CoV-2. SARS-CoV-2-specific NAb development can be induced in the blood of cattle. Pregnant cows produce NAbs upon immunization, and antibodies move into the colostrum immediately before calving. Here, we immunized cows with SARS-CoV-2 S1 receptor binding domain (RBD) protein in proper adjuvant solutions, followed by one boost with SARS-CoV-2 trimeric S protein and purified immunoglobulins from colostrum. We demonstrate that this preparation indeed blocks the interaction between the trimeric S protein and ACE2 in different in vitro assays. Moreover, we describe the formulation of purified immunoglobulin preparation into a nasal spray. When administered to human subjects, the formulation persisted on the nasal mucosa for at least 4 hours, as determined by a clinical study. Therefore, we are presenting a solution that shows great potential to serve as a prophylactic agent against SARS-CoV-2 infection as an additional measure to vaccination and wearing masks. Moreover, our technology allows for rapid and versatile adaptation for preparing prophylactic treatments against other diseases using the defined characteristics of antibody movement into the colostrum.
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spelling pubmed-91870602022-06-11 Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents Kangro, Kadri Kurašin, Mihhail Gildemann, Kiira Sankovski, Eve Žusinaite, Eva Lello, Laura Sandra Pert, Raini Kavak, Ants Poikalainen, Väino Lepasalu, Lembit Kuusk, Marilin Pau, Robin Piiskop, Sander Rom, Siimu Oltjer, Ruth Tiirik, Kairi Kogermann, Karin Plaas, Mario Tiirats, Toomas Aasmäe, Birgit Plaas, Mihkel Mumm, Karl Krinka, Dagni Talpsep, Ene Kadaja, Meelis Gerhold, Joachim M. Planken, Anu Tover, Andres Merits, Andres Männik, Andres Ustav, Mart Ustav, Mart PLoS One Research Article Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to impose a serious burden on health systems globally. Despite worldwide vaccination, social distancing and wearing masks, the spread of the virus is ongoing. One of the mechanisms by which neutralizing antibodies (NAbs) block virus entry into cells encompasses interaction inhibition between the cell surface receptor angiotensin-converting enzyme 2 (ACE2) and the spike (S) protein of SARS-CoV-2. SARS-CoV-2-specific NAb development can be induced in the blood of cattle. Pregnant cows produce NAbs upon immunization, and antibodies move into the colostrum immediately before calving. Here, we immunized cows with SARS-CoV-2 S1 receptor binding domain (RBD) protein in proper adjuvant solutions, followed by one boost with SARS-CoV-2 trimeric S protein and purified immunoglobulins from colostrum. We demonstrate that this preparation indeed blocks the interaction between the trimeric S protein and ACE2 in different in vitro assays. Moreover, we describe the formulation of purified immunoglobulin preparation into a nasal spray. When administered to human subjects, the formulation persisted on the nasal mucosa for at least 4 hours, as determined by a clinical study. Therefore, we are presenting a solution that shows great potential to serve as a prophylactic agent against SARS-CoV-2 infection as an additional measure to vaccination and wearing masks. Moreover, our technology allows for rapid and versatile adaptation for preparing prophylactic treatments against other diseases using the defined characteristics of antibody movement into the colostrum. Public Library of Science 2022-06-10 /pmc/articles/PMC9187060/ /pubmed/35687549 http://dx.doi.org/10.1371/journal.pone.0268806 Text en © 2022 Kangro et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kangro, Kadri
Kurašin, Mihhail
Gildemann, Kiira
Sankovski, Eve
Žusinaite, Eva
Lello, Laura Sandra
Pert, Raini
Kavak, Ants
Poikalainen, Väino
Lepasalu, Lembit
Kuusk, Marilin
Pau, Robin
Piiskop, Sander
Rom, Siimu
Oltjer, Ruth
Tiirik, Kairi
Kogermann, Karin
Plaas, Mario
Tiirats, Toomas
Aasmäe, Birgit
Plaas, Mihkel
Mumm, Karl
Krinka, Dagni
Talpsep, Ene
Kadaja, Meelis
Gerhold, Joachim M.
Planken, Anu
Tover, Andres
Merits, Andres
Männik, Andres
Ustav, Mart
Ustav, Mart
Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title_full Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title_fullStr Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title_full_unstemmed Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title_short Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents
title_sort bovine colostrum-derived antibodies against sars-cov-2 show great potential to serve as prophylactic agents
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187060/
https://www.ncbi.nlm.nih.gov/pubmed/35687549
http://dx.doi.org/10.1371/journal.pone.0268806
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