Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes

We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype.

Detalles Bibliográficos
Autores principales: Yoshina, Sawako, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187222/
https://www.ncbi.nlm.nih.gov/pubmed/35693894
http://dx.doi.org/10.17912/micropub.biology.000571
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author Yoshina, Sawako
Mitani, Shohei
author_facet Yoshina, Sawako
Mitani, Shohei
author_sort Yoshina, Sawako
collection PubMed
description We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype.
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spelling pubmed-91872222022-06-11 Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes Yoshina, Sawako Mitani, Shohei MicroPubl Biol New Methods We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype. Caltech Library 2022-05-18 /pmc/articles/PMC9187222/ /pubmed/35693894 http://dx.doi.org/10.17912/micropub.biology.000571 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Yoshina, Sawako
Mitani, Shohei
Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title_full Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title_fullStr Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title_full_unstemmed Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title_short Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
title_sort integration of multicopy extrachromosomal transgenes into defined loci without phenotypes
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187222/
https://www.ncbi.nlm.nih.gov/pubmed/35693894
http://dx.doi.org/10.17912/micropub.biology.000571
work_keys_str_mv AT yoshinasawako integrationofmulticopyextrachromosomaltransgenesintodefinedlociwithoutphenotypes
AT mitanishohei integrationofmulticopyextrachromosomaltransgenesintodefinedlociwithoutphenotypes

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