Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes

We show how presumably non-phenotypic loci can be used for integration sites of multi-copy extrachromosomal transgenes, using the CRISPR/Cas9 system. We used four loci, which show no apparent phenotype in our hands, as a model for any other loci with no phenotype.

Detalles Bibliográficos
Autores principales: Yoshina, Sawako, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187222/
https://www.ncbi.nlm.nih.gov/pubmed/35693894
http://dx.doi.org/10.17912/micropub.biology.000571

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187222/
https://www.ncbi.nlm.nih.gov/pubmed/35693894
http://dx.doi.org/10.17912/micropub.biology.000571

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