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Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants

The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the...

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Autores principales: Dünser, Kai, Schöller, Maria, Rößling, Ann-Kathrin, Löfke, Christian, Xiao, Nannan, Pařízková, Barbora, Melnik, Stanislav, Rodriguez-Franco, Marta, Stöger, Eva, Novák, Ondřej, Kleine-Vehn, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187339/
https://www.ncbi.nlm.nih.gov/pubmed/35686734
http://dx.doi.org/10.7554/eLife.75945
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author Dünser, Kai
Schöller, Maria
Rößling, Ann-Kathrin
Löfke, Christian
Xiao, Nannan
Pařízková, Barbora
Melnik, Stanislav
Rodriguez-Franco, Marta
Stöger, Eva
Novák, Ondřej
Kleine-Vehn, Jürgen
author_facet Dünser, Kai
Schöller, Maria
Rößling, Ann-Kathrin
Löfke, Christian
Xiao, Nannan
Pařízková, Barbora
Melnik, Stanislav
Rodriguez-Franco, Marta
Stöger, Eva
Novák, Ondřej
Kleine-Vehn, Jürgen
author_sort Dünser, Kai
collection PubMed
description The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the mechanisms that define vacuolar size. Here, we show that the cellular and vacuolar size expansions are coordinated. By developing a pharmacological tool, we enabled the investigation of membrane delivery to the vacuole during cellular expansion. Our data reveal that endocytic membrane sorting from the plasma membrane to the vacuole is enhanced in the course of rapid root cell expansion. While this ‘compromise’ mechanism may theoretically at first decelerate cell surface enlargements, it fuels vacuolar expansion and, thereby, ensures the coordinated augmentation of vacuolar occupancy in dynamically expanding plant cells.
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spelling pubmed-91873392022-06-11 Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants Dünser, Kai Schöller, Maria Rößling, Ann-Kathrin Löfke, Christian Xiao, Nannan Pařízková, Barbora Melnik, Stanislav Rodriguez-Franco, Marta Stöger, Eva Novák, Ondřej Kleine-Vehn, Jürgen eLife Cell Biology The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the mechanisms that define vacuolar size. Here, we show that the cellular and vacuolar size expansions are coordinated. By developing a pharmacological tool, we enabled the investigation of membrane delivery to the vacuole during cellular expansion. Our data reveal that endocytic membrane sorting from the plasma membrane to the vacuole is enhanced in the course of rapid root cell expansion. While this ‘compromise’ mechanism may theoretically at first decelerate cell surface enlargements, it fuels vacuolar expansion and, thereby, ensures the coordinated augmentation of vacuolar occupancy in dynamically expanding plant cells. eLife Sciences Publications, Ltd 2022-06-10 /pmc/articles/PMC9187339/ /pubmed/35686734 http://dx.doi.org/10.7554/eLife.75945 Text en © 2022, Dünser et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Dünser, Kai
Schöller, Maria
Rößling, Ann-Kathrin
Löfke, Christian
Xiao, Nannan
Pařízková, Barbora
Melnik, Stanislav
Rodriguez-Franco, Marta
Stöger, Eva
Novák, Ondřej
Kleine-Vehn, Jürgen
Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title_full Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title_fullStr Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title_full_unstemmed Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title_short Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
title_sort endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187339/
https://www.ncbi.nlm.nih.gov/pubmed/35686734
http://dx.doi.org/10.7554/eLife.75945
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