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Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats

In this study, we investigated whether transcutaneous electrical acupoint stimulation (TEAS) could improve cognitive function in VD rats by regulating PINK1/Parkin-mediated mitophagy. VD rat model was prepared by modified 2-vessel occlusion (2-VO) and randomly divided into four groups: Sham group (S...

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Detalles Bibliográficos
Autores principales: Hu, Ziwei, Wen, Tianshan, Hu, Ke, Wang, Rui, Su, Xinda, Ouyang, Wei, Zhou, Junjie, Wen, Youliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187477/
https://www.ncbi.nlm.nih.gov/pubmed/35692579
http://dx.doi.org/10.1155/2022/2810794
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author Hu, Ziwei
Wen, Tianshan
Hu, Ke
Wang, Rui
Su, Xinda
Ouyang, Wei
Zhou, Junjie
Wen, Youliang
author_facet Hu, Ziwei
Wen, Tianshan
Hu, Ke
Wang, Rui
Su, Xinda
Ouyang, Wei
Zhou, Junjie
Wen, Youliang
author_sort Hu, Ziwei
collection PubMed
description In this study, we investigated whether transcutaneous electrical acupoint stimulation (TEAS) could improve cognitive function in VD rats by regulating PINK1/Parkin-mediated mitophagy. VD rat model was prepared by modified 2-vessel occlusion (2-VO) and randomly divided into four groups: Sham group (Sham), Model group (Model), TEAS group (TEAS), and TEAS + 3-MA group (T +3 -MA). In the T +3 -MA group, autophagy inhibitor (3-MA) was injected into the lateral ventricle. After modeling, Y maze (YM), new object recognition test (NORT), Morris water maze (MWM), immunofluorescence, and Western blot were used to observe the effects of TEAS on VD rats. Behavioral experiments revealed that TEAS effectively improved the learning and memory ability of VD rats. Immunofluorescence results showed that TEAS could upregulate LC3 expression. Western blot results showed that TEAS upregulated the expression of PINK1, Parkin, and LC3-II, and downregulated the expression of LC3-I and p62 in VD rats. T +3 -MA group shows the opposite trend to TEAS group. This study demonstrates that TEAS ameliorates cognitive function through PINK1/Parkin-mediated mitophagy in VD rats.
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spelling pubmed-91874772022-06-11 Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats Hu, Ziwei Wen, Tianshan Hu, Ke Wang, Rui Su, Xinda Ouyang, Wei Zhou, Junjie Wen, Youliang Evid Based Complement Alternat Med Research Article In this study, we investigated whether transcutaneous electrical acupoint stimulation (TEAS) could improve cognitive function in VD rats by regulating PINK1/Parkin-mediated mitophagy. VD rat model was prepared by modified 2-vessel occlusion (2-VO) and randomly divided into four groups: Sham group (Sham), Model group (Model), TEAS group (TEAS), and TEAS + 3-MA group (T +3 -MA). In the T +3 -MA group, autophagy inhibitor (3-MA) was injected into the lateral ventricle. After modeling, Y maze (YM), new object recognition test (NORT), Morris water maze (MWM), immunofluorescence, and Western blot were used to observe the effects of TEAS on VD rats. Behavioral experiments revealed that TEAS effectively improved the learning and memory ability of VD rats. Immunofluorescence results showed that TEAS could upregulate LC3 expression. Western blot results showed that TEAS upregulated the expression of PINK1, Parkin, and LC3-II, and downregulated the expression of LC3-I and p62 in VD rats. T +3 -MA group shows the opposite trend to TEAS group. This study demonstrates that TEAS ameliorates cognitive function through PINK1/Parkin-mediated mitophagy in VD rats. Hindawi 2022-06-03 /pmc/articles/PMC9187477/ /pubmed/35692579 http://dx.doi.org/10.1155/2022/2810794 Text en Copyright © 2022 Ziwei Hu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hu, Ziwei
Wen, Tianshan
Hu, Ke
Wang, Rui
Su, Xinda
Ouyang, Wei
Zhou, Junjie
Wen, Youliang
Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title_full Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title_fullStr Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title_full_unstemmed Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title_short Transcutaneous Electrical Acupoint Stimulation Ameliorates Cognitive Function through PINK1/Parkin Mediated Mitophagy in VD Rats
title_sort transcutaneous electrical acupoint stimulation ameliorates cognitive function through pink1/parkin mediated mitophagy in vd rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187477/
https://www.ncbi.nlm.nih.gov/pubmed/35692579
http://dx.doi.org/10.1155/2022/2810794
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