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Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation
Malaria long-term elimination depends on parasite transmission control. Plasmodium sexual stage maturation in the mosquito, including egress from the host erythrocyte, is one of the prime targets for transmission-blocking interventions. This work aims to identify candidate molecules potentially invo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187623/ https://www.ncbi.nlm.nih.gov/pubmed/35689013 http://dx.doi.org/10.1038/s41598-022-13415-7 |
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author | Grasso, Felicia Fratini, Federica Albanese, Tanino Giuseppe Mochi, Stefania Ciardo, Mariagrazia Pace, Tomasino Ponzi, Marta Pizzi, Elisabetta Olivieri, Anna |
author_facet | Grasso, Felicia Fratini, Federica Albanese, Tanino Giuseppe Mochi, Stefania Ciardo, Mariagrazia Pace, Tomasino Ponzi, Marta Pizzi, Elisabetta Olivieri, Anna |
author_sort | Grasso, Felicia |
collection | PubMed |
description | Malaria long-term elimination depends on parasite transmission control. Plasmodium sexual stage maturation in the mosquito, including egress from the host erythrocyte, is one of the prime targets for transmission-blocking interventions. This work aims to identify candidate molecules potentially involved in gamete emergence from the host erythrocyte, as novel transmission blocking targets. We analyzed by quantitative mass spectrometry the proteins released/secreted by purified Plasmodium falciparum gametocytes upon induction of gametogenesis. The proteome obtained showed a good overlap (74%) with the one previously characterized in similar conditions from gametocytes of the rodent malaria parasite P. berghei. Four candidates were selected based on comparative analysis of their abundance values in released vs total gametocyte proteome. We also characterized the P. falciparum orthologue of the microgamete surface protein (MiGS), a marker of male gametocyte secretory vesicles in murine models of malaria. The findings of this study reveal that all the selected candidate proteins are expressed in both genders and localize to vesicle-like structures that respond to gametogenesis stimuli. This result, together with the fact that the selected proteins are released during gamete emergence in both Plasmodium species, makes them interesting candidates for future functional studies to investigate their potential role in the gametogenesis process. |
format | Online Article Text |
id | pubmed-9187623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91876232022-06-12 Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation Grasso, Felicia Fratini, Federica Albanese, Tanino Giuseppe Mochi, Stefania Ciardo, Mariagrazia Pace, Tomasino Ponzi, Marta Pizzi, Elisabetta Olivieri, Anna Sci Rep Article Malaria long-term elimination depends on parasite transmission control. Plasmodium sexual stage maturation in the mosquito, including egress from the host erythrocyte, is one of the prime targets for transmission-blocking interventions. This work aims to identify candidate molecules potentially involved in gamete emergence from the host erythrocyte, as novel transmission blocking targets. We analyzed by quantitative mass spectrometry the proteins released/secreted by purified Plasmodium falciparum gametocytes upon induction of gametogenesis. The proteome obtained showed a good overlap (74%) with the one previously characterized in similar conditions from gametocytes of the rodent malaria parasite P. berghei. Four candidates were selected based on comparative analysis of their abundance values in released vs total gametocyte proteome. We also characterized the P. falciparum orthologue of the microgamete surface protein (MiGS), a marker of male gametocyte secretory vesicles in murine models of malaria. The findings of this study reveal that all the selected candidate proteins are expressed in both genders and localize to vesicle-like structures that respond to gametogenesis stimuli. This result, together with the fact that the selected proteins are released during gamete emergence in both Plasmodium species, makes them interesting candidates for future functional studies to investigate their potential role in the gametogenesis process. Nature Publishing Group UK 2022-06-10 /pmc/articles/PMC9187623/ /pubmed/35689013 http://dx.doi.org/10.1038/s41598-022-13415-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Grasso, Felicia Fratini, Federica Albanese, Tanino Giuseppe Mochi, Stefania Ciardo, Mariagrazia Pace, Tomasino Ponzi, Marta Pizzi, Elisabetta Olivieri, Anna Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title | Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title_full | Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title_fullStr | Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title_full_unstemmed | Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title_short | Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation |
title_sort | identification and preliminary characterization of plasmodium falciparum proteins secreted upon gamete formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187623/ https://www.ncbi.nlm.nih.gov/pubmed/35689013 http://dx.doi.org/10.1038/s41598-022-13415-7 |
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