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Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products

Traditional herbal medicine has long been practiced as a method of health care in many countries worldwide. The usage of herbal products has been increasing and is expected to continue to do so in the future. However, admixture and adulteration are concerns regarding the quality of herbal medicine,...

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Autores principales: Urumarudappa, Santhosh Kumar J., Tungphatthong, Chayapol, Jaipaew, Jirayut, Pornputtapong, Natapol, Pakdeesattayapong, Duangkamol, Vimolmangkang, Sornkanok, Sukrong, Suchada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187672/
https://www.ncbi.nlm.nih.gov/pubmed/35688884
http://dx.doi.org/10.1038/s41598-022-13287-x
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author Urumarudappa, Santhosh Kumar J.
Tungphatthong, Chayapol
Jaipaew, Jirayut
Pornputtapong, Natapol
Pakdeesattayapong, Duangkamol
Vimolmangkang, Sornkanok
Sukrong, Suchada
author_facet Urumarudappa, Santhosh Kumar J.
Tungphatthong, Chayapol
Jaipaew, Jirayut
Pornputtapong, Natapol
Pakdeesattayapong, Duangkamol
Vimolmangkang, Sornkanok
Sukrong, Suchada
author_sort Urumarudappa, Santhosh Kumar J.
collection PubMed
description Traditional herbal medicine has long been practiced as a method of health care in many countries worldwide. The usage of herbal products has been increasing and is expected to continue to do so in the future. However, admixture and adulteration are concerns regarding the quality of herbal medicine, including its safety and efficacy. We aimed to develop a reference DNA barcode library of plants listed in the Thai Herbal Pharmacopoeia (THP) and Monographs of Selected Thai Materia Medica (TMM) (n = 101 plant species) using four core barcode regions, namely, the ITS2, matK, rbcL and trnH-psbA intergenic spacer regions, for authentication of the plant origin of raw materials and herbal products. Checking sequences from samples obtained from local markets and the Thai Food and Drug Administration (Thai FDA) against our digital reference DNA barcode system revealed the authenticity of eighteen out of twenty tested samples as claimed on their labels. Two samples, no. 3 and 13, were not Cyanthillium cinereum (L.) H.Rob. and Pueraria candollei Wall. ex Benth. as claimed, respectively. They were recognized as Emilia sonchifolia (L.) DC. and Butea superba (Roxb.), respectively. Hence, it is important for the Thai FDA or regulatory agencies to immediately initiate strict enforcement for the development of pharmacopoeial standards as well as revisions or modifications of available regulatory guidelines and to implement close monitoring for the quality control of herbal products in terms of authentication before they enter the herbal market. The centralized digital reference DNA barcode database developed here could play a very important role in monitoring or checking the authenticity of medicinal plants.
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spelling pubmed-91876722022-06-12 Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products Urumarudappa, Santhosh Kumar J. Tungphatthong, Chayapol Jaipaew, Jirayut Pornputtapong, Natapol Pakdeesattayapong, Duangkamol Vimolmangkang, Sornkanok Sukrong, Suchada Sci Rep Article Traditional herbal medicine has long been practiced as a method of health care in many countries worldwide. The usage of herbal products has been increasing and is expected to continue to do so in the future. However, admixture and adulteration are concerns regarding the quality of herbal medicine, including its safety and efficacy. We aimed to develop a reference DNA barcode library of plants listed in the Thai Herbal Pharmacopoeia (THP) and Monographs of Selected Thai Materia Medica (TMM) (n = 101 plant species) using four core barcode regions, namely, the ITS2, matK, rbcL and trnH-psbA intergenic spacer regions, for authentication of the plant origin of raw materials and herbal products. Checking sequences from samples obtained from local markets and the Thai Food and Drug Administration (Thai FDA) against our digital reference DNA barcode system revealed the authenticity of eighteen out of twenty tested samples as claimed on their labels. Two samples, no. 3 and 13, were not Cyanthillium cinereum (L.) H.Rob. and Pueraria candollei Wall. ex Benth. as claimed, respectively. They were recognized as Emilia sonchifolia (L.) DC. and Butea superba (Roxb.), respectively. Hence, it is important for the Thai FDA or regulatory agencies to immediately initiate strict enforcement for the development of pharmacopoeial standards as well as revisions or modifications of available regulatory guidelines and to implement close monitoring for the quality control of herbal products in terms of authentication before they enter the herbal market. The centralized digital reference DNA barcode database developed here could play a very important role in monitoring or checking the authenticity of medicinal plants. Nature Publishing Group UK 2022-06-10 /pmc/articles/PMC9187672/ /pubmed/35688884 http://dx.doi.org/10.1038/s41598-022-13287-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Urumarudappa, Santhosh Kumar J.
Tungphatthong, Chayapol
Jaipaew, Jirayut
Pornputtapong, Natapol
Pakdeesattayapong, Duangkamol
Vimolmangkang, Sornkanok
Sukrong, Suchada
Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title_full Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title_fullStr Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title_full_unstemmed Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title_short Development of a DNA barcode library of plants in the Thai Herbal Pharmacopoeia and Monographs for authentication of herbal products
title_sort development of a dna barcode library of plants in the thai herbal pharmacopoeia and monographs for authentication of herbal products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187672/
https://www.ncbi.nlm.nih.gov/pubmed/35688884
http://dx.doi.org/10.1038/s41598-022-13287-x
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