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Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure
Heart failure (HF), as the leading cause of death, is continuing to increase along with the aging of the general population all over the world. Identification of diagnostic biomarkers for early detection of HF is considered as the most effective way to reduce the risk and mortality. Herein, we colle...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188190/ https://www.ncbi.nlm.nih.gov/pubmed/35690861 http://dx.doi.org/10.1186/s12967-022-03465-w |
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author | Qian, Lu Zhao, Qian Yu, Ping Lü, Jinhui Guo, Yuefan Gong, Xin Ding, Yuanyuan Yu, Shanshan Fan, Lieying Fan, Huimin Zhang, Yuzhen Liu, Zhongmin Sheng, Hongzhuan Yu, Zuoren |
author_facet | Qian, Lu Zhao, Qian Yu, Ping Lü, Jinhui Guo, Yuefan Gong, Xin Ding, Yuanyuan Yu, Shanshan Fan, Lieying Fan, Huimin Zhang, Yuzhen Liu, Zhongmin Sheng, Hongzhuan Yu, Zuoren |
author_sort | Qian, Lu |
collection | PubMed |
description | Heart failure (HF), as the leading cause of death, is continuing to increase along with the aging of the general population all over the world. Identification of diagnostic biomarkers for early detection of HF is considered as the most effective way to reduce the risk and mortality. Herein, we collected plasma samples from HF patients (n = 40) before and after medical therapy to determine the change of circulating miRNAs through a quantitative real-time PCR (QRT-PCR)-based miRNA screening analysis. miR-30a-5p and miR-654-5p were identified as the most significantly changed miRNAs in the plasma of patients upon treatment. In consistence, miR-30a-5p showed upregulation and miR-654-5p showed downregulation in the circulation of 30 HF patients, compared to 15 normal controls in the training phase, from which a two-circulating miRNA model was developed for HF diagnosis. Next, we performed the model validation using an independent cohort including 50 HF patients and 30 controls. As high as 98.75% of sensitivity and 95.00% of specificity were achieved. A comparison between the miRNA model and NT-pro BNP in diagnostic accuracy of HF indicated an upward trend of the miRNA model. Moreover, change of the two miRNAs was further verified in association with the therapeutic effect of HF patients, in which miR-30a-5p showed decrease while miR-654-5p showed increase in the plasma of patients after LVAD implantation. In conclusion, the current study not only identified circulating miR-654-5p for the first time as a novel biomarker of HF, but also developed a novel 2-circulating miRNA model with promising potentials for diagnosis and prognosis of HF patients, and in association with therapeutic effects as well. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03465-w. |
format | Online Article Text |
id | pubmed-9188190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91881902022-06-12 Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure Qian, Lu Zhao, Qian Yu, Ping Lü, Jinhui Guo, Yuefan Gong, Xin Ding, Yuanyuan Yu, Shanshan Fan, Lieying Fan, Huimin Zhang, Yuzhen Liu, Zhongmin Sheng, Hongzhuan Yu, Zuoren J Transl Med Research Heart failure (HF), as the leading cause of death, is continuing to increase along with the aging of the general population all over the world. Identification of diagnostic biomarkers for early detection of HF is considered as the most effective way to reduce the risk and mortality. Herein, we collected plasma samples from HF patients (n = 40) before and after medical therapy to determine the change of circulating miRNAs through a quantitative real-time PCR (QRT-PCR)-based miRNA screening analysis. miR-30a-5p and miR-654-5p were identified as the most significantly changed miRNAs in the plasma of patients upon treatment. In consistence, miR-30a-5p showed upregulation and miR-654-5p showed downregulation in the circulation of 30 HF patients, compared to 15 normal controls in the training phase, from which a two-circulating miRNA model was developed for HF diagnosis. Next, we performed the model validation using an independent cohort including 50 HF patients and 30 controls. As high as 98.75% of sensitivity and 95.00% of specificity were achieved. A comparison between the miRNA model and NT-pro BNP in diagnostic accuracy of HF indicated an upward trend of the miRNA model. Moreover, change of the two miRNAs was further verified in association with the therapeutic effect of HF patients, in which miR-30a-5p showed decrease while miR-654-5p showed increase in the plasma of patients after LVAD implantation. In conclusion, the current study not only identified circulating miR-654-5p for the first time as a novel biomarker of HF, but also developed a novel 2-circulating miRNA model with promising potentials for diagnosis and prognosis of HF patients, and in association with therapeutic effects as well. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12967-022-03465-w. BioMed Central 2022-06-11 /pmc/articles/PMC9188190/ /pubmed/35690861 http://dx.doi.org/10.1186/s12967-022-03465-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Qian, Lu Zhao, Qian Yu, Ping Lü, Jinhui Guo, Yuefan Gong, Xin Ding, Yuanyuan Yu, Shanshan Fan, Lieying Fan, Huimin Zhang, Yuzhen Liu, Zhongmin Sheng, Hongzhuan Yu, Zuoren Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title | Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title_full | Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title_fullStr | Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title_full_unstemmed | Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title_short | Diagnostic potential of a circulating miRNA model associated with therapeutic effect in heart failure |
title_sort | diagnostic potential of a circulating mirna model associated with therapeutic effect in heart failure |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188190/ https://www.ncbi.nlm.nih.gov/pubmed/35690861 http://dx.doi.org/10.1186/s12967-022-03465-w |
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