Cargando…
Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior
BACKGROUND: Human gingival fibroblast (HGF-1) cells in the connective tissue provide an effective barrier between the alveolar bone and the oral environment. Cement margins of restorations with intrasulcular preparation or cemented implant restorations are in contact with HGF cells. However, it is u...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188230/ https://www.ncbi.nlm.nih.gov/pubmed/35690829 http://dx.doi.org/10.1186/s13005-022-00323-4 |
_version_ | 1784725328804970496 |
---|---|
author | Rohr, Nadja Baumann, Celina Märtin, Sabrina Zitzmann, Nicola U. |
author_facet | Rohr, Nadja Baumann, Celina Märtin, Sabrina Zitzmann, Nicola U. |
author_sort | Rohr, Nadja |
collection | PubMed |
description | BACKGROUND: Human gingival fibroblast (HGF-1) cells in the connective tissue provide an effective barrier between the alveolar bone and the oral environment. Cement margins of restorations with intrasulcular preparation or cemented implant restorations are in contact with HGF cells. However, it is unknown to what extend the cement surface finish affects the behavior of HGF cells. The purpose of this study was to compare the behavior of HGF-1 cells in contact with two different resin composite cements with three different surface treatments after light-curing and autopolymerization, respectively. METHODS: Disks of one adhesive (Multilink Automix, Ivoclar Vivadent [MLA]) and one self-adhesive (RelyX Unicem 2 Automix, 3 M [RUN]) resin composite cement were either light-cured or autopolymerized. Specimen surfaces were prepared with the oxygen inhibition layer intact, polished with P2500-grit silicon carbide paper or treated with a scaler. Cells were cultivated on the specimens for 24 h. Viability assay was performed, and cell morphology was examined with scanning electron microscopy. Additionally, roughness parameters of the specimen were analyzed with a 3D laser scanning microscope. Three-way ANOVA was applied to determine the effect of cement material, curing mode and surface treatment (a = 0.05). RESULTS: Overall, cement material (p = 0.031), curing mode (p = 0.001), and surface treatment (p < 0.001) significantly affected relative cell viability of HGF. The autopolymerized specimen with the oxygen inhibition layer left intact displayed the lowest relative cell viability (MLA 25.7%, RUN 46.6%). Removal of the oxygen inhibition layer with a scaler increased cell viability but also resulted in higher surface roughness values. CONCLUSIONS: HGF cell viability is affected by the surface treatment and the curing mode. The oxygen inhibition layer is an inhibitory factor for the viability of HGF cells. Autopolymerization enhances the cytotoxic potential of the oxygen inhibition layer. |
format | Online Article Text |
id | pubmed-9188230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91882302022-06-12 Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior Rohr, Nadja Baumann, Celina Märtin, Sabrina Zitzmann, Nicola U. Head Face Med Research BACKGROUND: Human gingival fibroblast (HGF-1) cells in the connective tissue provide an effective barrier between the alveolar bone and the oral environment. Cement margins of restorations with intrasulcular preparation or cemented implant restorations are in contact with HGF cells. However, it is unknown to what extend the cement surface finish affects the behavior of HGF cells. The purpose of this study was to compare the behavior of HGF-1 cells in contact with two different resin composite cements with three different surface treatments after light-curing and autopolymerization, respectively. METHODS: Disks of one adhesive (Multilink Automix, Ivoclar Vivadent [MLA]) and one self-adhesive (RelyX Unicem 2 Automix, 3 M [RUN]) resin composite cement were either light-cured or autopolymerized. Specimen surfaces were prepared with the oxygen inhibition layer intact, polished with P2500-grit silicon carbide paper or treated with a scaler. Cells were cultivated on the specimens for 24 h. Viability assay was performed, and cell morphology was examined with scanning electron microscopy. Additionally, roughness parameters of the specimen were analyzed with a 3D laser scanning microscope. Three-way ANOVA was applied to determine the effect of cement material, curing mode and surface treatment (a = 0.05). RESULTS: Overall, cement material (p = 0.031), curing mode (p = 0.001), and surface treatment (p < 0.001) significantly affected relative cell viability of HGF. The autopolymerized specimen with the oxygen inhibition layer left intact displayed the lowest relative cell viability (MLA 25.7%, RUN 46.6%). Removal of the oxygen inhibition layer with a scaler increased cell viability but also resulted in higher surface roughness values. CONCLUSIONS: HGF cell viability is affected by the surface treatment and the curing mode. The oxygen inhibition layer is an inhibitory factor for the viability of HGF cells. Autopolymerization enhances the cytotoxic potential of the oxygen inhibition layer. BioMed Central 2022-06-11 /pmc/articles/PMC9188230/ /pubmed/35690829 http://dx.doi.org/10.1186/s13005-022-00323-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Rohr, Nadja Baumann, Celina Märtin, Sabrina Zitzmann, Nicola U. Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title | Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title_full | Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title_fullStr | Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title_full_unstemmed | Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title_short | Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
title_sort | influence of surface treatment and curing mode of resin composite cements on fibroblast behavior |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188230/ https://www.ncbi.nlm.nih.gov/pubmed/35690829 http://dx.doi.org/10.1186/s13005-022-00323-4 |
work_keys_str_mv | AT rohrnadja influenceofsurfacetreatmentandcuringmodeofresincompositecementsonfibroblastbehavior AT baumanncelina influenceofsurfacetreatmentandcuringmodeofresincompositecementsonfibroblastbehavior AT martinsabrina influenceofsurfacetreatmentandcuringmodeofresincompositecementsonfibroblastbehavior AT zitzmannnicolau influenceofsurfacetreatmentandcuringmodeofresincompositecementsonfibroblastbehavior |