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Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system

This paper describes repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with a microfluidic heating system. Biochemical sensors that respond to chemical compounds and produce detectable signals have a critical role in many aspects of modern society....

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Autores principales: Yoshida, Koki, Hayashi, Tomoki, Takinoue, Masahiro, Onoe, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188593/
https://www.ncbi.nlm.nih.gov/pubmed/35690676
http://dx.doi.org/10.1038/s41598-022-13970-z
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author Yoshida, Koki
Hayashi, Tomoki
Takinoue, Masahiro
Onoe, Hiroaki
author_facet Yoshida, Koki
Hayashi, Tomoki
Takinoue, Masahiro
Onoe, Hiroaki
author_sort Yoshida, Koki
collection PubMed
description This paper describes repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with a microfluidic heating system. Biochemical sensors that respond to chemical compounds and produce detectable signals have a critical role in many aspects of modern society. In particular, the repeatable measurement of environmental information such as toxic substances including Ag(+) ions could be expected to improve the environment. The DNA aptamer is an attractive candidate because of the stability and the selectivity of binding to chemicals. However, previous DNA aptamer biochemical sensors could not measure repeatedly because those sensors did not have initializing functions. To overcome this challenge, we proposed a DNA aptamer-linked hydrogel biochemical sensor integrated with the microfluidic heating system enabling repeatable detection of Ag(+) ions. The binding Ag(+) ions are dissociated by heating and flushing through the integrated microfluidic heating device. The DNA aptamer-linked hydrogel had the capability to detect a wide range of Ag(+) ion concentrations (10(−5)–10 mM) including a toxic range for various aquatic organisms. Finally, we demonstrated the repeatable detection of the Ag(+) ions. These results indicated that our proposed biochemical sensor is expected to use for long-term monitoring with high stability in ambient temperature and low power consumption.
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spelling pubmed-91885932022-06-13 Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system Yoshida, Koki Hayashi, Tomoki Takinoue, Masahiro Onoe, Hiroaki Sci Rep Article This paper describes repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with a microfluidic heating system. Biochemical sensors that respond to chemical compounds and produce detectable signals have a critical role in many aspects of modern society. In particular, the repeatable measurement of environmental information such as toxic substances including Ag(+) ions could be expected to improve the environment. The DNA aptamer is an attractive candidate because of the stability and the selectivity of binding to chemicals. However, previous DNA aptamer biochemical sensors could not measure repeatedly because those sensors did not have initializing functions. To overcome this challenge, we proposed a DNA aptamer-linked hydrogel biochemical sensor integrated with the microfluidic heating system enabling repeatable detection of Ag(+) ions. The binding Ag(+) ions are dissociated by heating and flushing through the integrated microfluidic heating device. The DNA aptamer-linked hydrogel had the capability to detect a wide range of Ag(+) ion concentrations (10(−5)–10 mM) including a toxic range for various aquatic organisms. Finally, we demonstrated the repeatable detection of the Ag(+) ions. These results indicated that our proposed biochemical sensor is expected to use for long-term monitoring with high stability in ambient temperature and low power consumption. Nature Publishing Group UK 2022-06-11 /pmc/articles/PMC9188593/ /pubmed/35690676 http://dx.doi.org/10.1038/s41598-022-13970-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yoshida, Koki
Hayashi, Tomoki
Takinoue, Masahiro
Onoe, Hiroaki
Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title_full Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title_fullStr Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title_full_unstemmed Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title_short Repeatable detection of Ag(+) ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
title_sort repeatable detection of ag(+) ions using a dna aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188593/
https://www.ncbi.nlm.nih.gov/pubmed/35690676
http://dx.doi.org/10.1038/s41598-022-13970-z
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