Protocol to isolate and assess spike protein cleavage in SARS-CoV-2 variants obtained from clinical COVID-19 samples

For efficient cell entry, SARS-CoV-2 spike protein needs to be cleaved by cellular proteases. Here, we present a comprehensive protocol to assess SARS-CoV-2 spike protein cleavage in viral supernatants from SARS-CoV-2-infected cells. We also include a previous step of SARS-CoV-2 isolation from nasop...

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Detalles Bibliográficos
Autores principales: Escalera, Alba, García-Sastre, Adolfo, Aydillo, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189002/
https://www.ncbi.nlm.nih.gov/pubmed/35776647
http://dx.doi.org/10.1016/j.xpro.2022.101502

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189002/
https://www.ncbi.nlm.nih.gov/pubmed/35776647
http://dx.doi.org/10.1016/j.xpro.2022.101502

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