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Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies
Small near-infrared (NIR) fluorescent proteins (FPs) are much needed as protein tags for imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which brightly fluoresces in mammalian cells and enables deep-brain imaging. By exploring miRFP670nano3 as an internal tag, we engineered...
Autores principales: | Oliinyk, Olena S., Baloban, Mikhail, Clark, Charles L., Carey, Erin, Pletnev, Sergei, Nimmerjahn, Axel, Verkhusha, Vladislav V. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189029/ https://www.ncbi.nlm.nih.gov/pubmed/35606446 http://dx.doi.org/10.1038/s41592-022-01467-6 |
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