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Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury

Recent studies showed that RNA N6-methyladenosine (m6A) plays an important role in neurological diseases. We used methylated RNA immunoprecipitation sequencing (MeRIP-Seq) technology to generate the m6A modification map after traumatic spinal cord injury (TSCI). A total of 2,609 differential m6A pea...

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Autores principales: Yu, Jiangtao, Chen, Haihua, Ma, Haoli, Zhang, Zhongxiang, Zhu, Xiaolu, Wang, Pengcheng, Liu, Ruining, Jin, Xiaoqing, Zhao, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189298/
https://www.ncbi.nlm.nih.gov/pubmed/35706691
http://dx.doi.org/10.3389/fnins.2022.848119
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author Yu, Jiangtao
Chen, Haihua
Ma, Haoli
Zhang, Zhongxiang
Zhu, Xiaolu
Wang, Pengcheng
Liu, Ruining
Jin, Xiaoqing
Zhao, Yan
author_facet Yu, Jiangtao
Chen, Haihua
Ma, Haoli
Zhang, Zhongxiang
Zhu, Xiaolu
Wang, Pengcheng
Liu, Ruining
Jin, Xiaoqing
Zhao, Yan
author_sort Yu, Jiangtao
collection PubMed
description Recent studies showed that RNA N6-methyladenosine (m6A) plays an important role in neurological diseases. We used methylated RNA immunoprecipitation sequencing (MeRIP-Seq) technology to generate the m6A modification map after traumatic spinal cord injury (TSCI). A total of 2,609 differential m6A peaks were identified after TSCI. Our RNA sequencing results after TSCI showed 4,206 genes with significantly altered expression. Cross-link analysis of m6A sequencing results and RNA sequencing results showed that 141 hyper-methylated genes were upregulated, 53 hyper-methylated genes were downregulated, 57 hypo-methylated genes were upregulated, and 197 hypo-methylated genes were downregulated. Among these, the important inflammatory response factor Tlr4 and the important member of the neurotrophin family Ngf were both upregulated and hyper-methylated after TSCI. This study provides that in the future, the epigenetic modifications of the genes could be used as an indicator of TSCI.
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spelling pubmed-91892982022-06-14 Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury Yu, Jiangtao Chen, Haihua Ma, Haoli Zhang, Zhongxiang Zhu, Xiaolu Wang, Pengcheng Liu, Ruining Jin, Xiaoqing Zhao, Yan Front Neurosci Neuroscience Recent studies showed that RNA N6-methyladenosine (m6A) plays an important role in neurological diseases. We used methylated RNA immunoprecipitation sequencing (MeRIP-Seq) technology to generate the m6A modification map after traumatic spinal cord injury (TSCI). A total of 2,609 differential m6A peaks were identified after TSCI. Our RNA sequencing results after TSCI showed 4,206 genes with significantly altered expression. Cross-link analysis of m6A sequencing results and RNA sequencing results showed that 141 hyper-methylated genes were upregulated, 53 hyper-methylated genes were downregulated, 57 hypo-methylated genes were upregulated, and 197 hypo-methylated genes were downregulated. Among these, the important inflammatory response factor Tlr4 and the important member of the neurotrophin family Ngf were both upregulated and hyper-methylated after TSCI. This study provides that in the future, the epigenetic modifications of the genes could be used as an indicator of TSCI. Frontiers Media S.A. 2022-05-30 /pmc/articles/PMC9189298/ /pubmed/35706691 http://dx.doi.org/10.3389/fnins.2022.848119 Text en Copyright © 2022 Yu, Chen, Ma, Zhang, Zhu, Wang, Liu, Jin and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Yu, Jiangtao
Chen, Haihua
Ma, Haoli
Zhang, Zhongxiang
Zhu, Xiaolu
Wang, Pengcheng
Liu, Ruining
Jin, Xiaoqing
Zhao, Yan
Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title_full Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title_fullStr Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title_full_unstemmed Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title_short Transcriptome-Wide N6-Methyladenosine Methylome Alteration in the Rat Spinal Cord After Acute Traumatic Spinal Cord Injury
title_sort transcriptome-wide n6-methyladenosine methylome alteration in the rat spinal cord after acute traumatic spinal cord injury
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189298/
https://www.ncbi.nlm.nih.gov/pubmed/35706691
http://dx.doi.org/10.3389/fnins.2022.848119
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