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Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides

Porcine reproductive and respiratory syndrome (PRRS) is a widespread disease with great economic importance in the pig industry. Although vaccines against the PRRS virus (PRRSV) have been employed for more than 20 years, differentiating infected from vaccinated animals remains challenging. In this s...

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Autores principales: Li, Dong-Yan, Cui, Xing-Yang, Huang, Xin-Yi, Hu, Yue, Tian, Xiao-Xiao, Wang, Tao, Yang, Yong-Bo, Wang, Qian, Tian, Zhi-Jun, Cai, Xue-Hui, An, Tong-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189411/
https://www.ncbi.nlm.nih.gov/pubmed/35706603
http://dx.doi.org/10.3389/fvets.2022.902822
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author Li, Dong-Yan
Cui, Xing-Yang
Huang, Xin-Yi
Hu, Yue
Tian, Xiao-Xiao
Wang, Tao
Yang, Yong-Bo
Wang, Qian
Tian, Zhi-Jun
Cai, Xue-Hui
An, Tong-Qing
author_facet Li, Dong-Yan
Cui, Xing-Yang
Huang, Xin-Yi
Hu, Yue
Tian, Xiao-Xiao
Wang, Tao
Yang, Yong-Bo
Wang, Qian
Tian, Zhi-Jun
Cai, Xue-Hui
An, Tong-Qing
author_sort Li, Dong-Yan
collection PubMed
description Porcine reproductive and respiratory syndrome (PRRS) is a widespread disease with great economic importance in the pig industry. Although vaccines against the PRRS virus (PRRSV) have been employed for more than 20 years, differentiating infected from vaccinated animals remains challenging. In this study, all 907 non-structural protein 2 (NSP2) full-length sequences of PRRSV-2 available from GenBank were aligned. Two peptides, at positions 562–627 (m1B) and 749–813 (m2B) of NSP2, were selected, and their potential for use in differential diagnosis was assessed. Both m1B and m2B were recognized by PRRSV-positive pig serum in peptide-coated enzyme-linked immunosorbent assays. Further epitope identification yielded five overlapping short peptides for the immunodominant regions of m1B and m2B. Using the infectious clone of PRRSV HuN4-F112 as a template, the deletion mutants, rHuN4-F112-m1B, rHuN4-F112-m2B, and rHuN4-F112-C5-m1B-m2B, were generated and successfully rescued in Marc-145 cells. Growth kinetics revealed that the deletion of m1B and m2B did not significantly affect virus replication. Hence, m1B and m2B show potential as molecular markers for developing a PRRSV vaccine.
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spelling pubmed-91894112022-06-14 Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides Li, Dong-Yan Cui, Xing-Yang Huang, Xin-Yi Hu, Yue Tian, Xiao-Xiao Wang, Tao Yang, Yong-Bo Wang, Qian Tian, Zhi-Jun Cai, Xue-Hui An, Tong-Qing Front Vet Sci Veterinary Science Porcine reproductive and respiratory syndrome (PRRS) is a widespread disease with great economic importance in the pig industry. Although vaccines against the PRRS virus (PRRSV) have been employed for more than 20 years, differentiating infected from vaccinated animals remains challenging. In this study, all 907 non-structural protein 2 (NSP2) full-length sequences of PRRSV-2 available from GenBank were aligned. Two peptides, at positions 562–627 (m1B) and 749–813 (m2B) of NSP2, were selected, and their potential for use in differential diagnosis was assessed. Both m1B and m2B were recognized by PRRSV-positive pig serum in peptide-coated enzyme-linked immunosorbent assays. Further epitope identification yielded five overlapping short peptides for the immunodominant regions of m1B and m2B. Using the infectious clone of PRRSV HuN4-F112 as a template, the deletion mutants, rHuN4-F112-m1B, rHuN4-F112-m2B, and rHuN4-F112-C5-m1B-m2B, were generated and successfully rescued in Marc-145 cells. Growth kinetics revealed that the deletion of m1B and m2B did not significantly affect virus replication. Hence, m1B and m2B show potential as molecular markers for developing a PRRSV vaccine. Frontiers Media S.A. 2022-05-30 /pmc/articles/PMC9189411/ /pubmed/35706603 http://dx.doi.org/10.3389/fvets.2022.902822 Text en Copyright © 2022 Li, Cui, Huang, Hu, Tian, Wang, Yang, Wang, Tian, Cai and An. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Dong-Yan
Cui, Xing-Yang
Huang, Xin-Yi
Hu, Yue
Tian, Xiao-Xiao
Wang, Tao
Yang, Yong-Bo
Wang, Qian
Tian, Zhi-Jun
Cai, Xue-Hui
An, Tong-Qing
Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title_full Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title_fullStr Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title_full_unstemmed Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title_short Characterization of Two Immunodominant Antigenic Peptides in NSP2 of PRRSV-2 and Generation of a Marker PRRSV Strain Based on the Peptides
title_sort characterization of two immunodominant antigenic peptides in nsp2 of prrsv-2 and generation of a marker prrsv strain based on the peptides
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189411/
https://www.ncbi.nlm.nih.gov/pubmed/35706603
http://dx.doi.org/10.3389/fvets.2022.902822
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