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A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator

Intracellular vesicles such as lysosomes contain micromolar to millimolar concentrations of Zn(2+), and disturbing lysosomal Zn(2+) homeostasis via lysosomal Zn(2+) release leads to mitochondria damage and consequent lytic cell death. Methods have been developed to image cellular Zn(2+) dynamics. He...

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Autores principales: Gu, Mingxue, Hu, Meiqin, Minckley, Taylor, Pinchi, Prateeksunder, Xu, Haoxing, Qin, Yan, Du, Wanlu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189625/
https://www.ncbi.nlm.nih.gov/pubmed/35707683
http://dx.doi.org/10.1016/j.xpro.2022.101453
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author Gu, Mingxue
Hu, Meiqin
Minckley, Taylor
Pinchi, Prateeksunder
Xu, Haoxing
Qin, Yan
Du, Wanlu
author_facet Gu, Mingxue
Hu, Meiqin
Minckley, Taylor
Pinchi, Prateeksunder
Xu, Haoxing
Qin, Yan
Du, Wanlu
author_sort Gu, Mingxue
collection PubMed
description Intracellular vesicles such as lysosomes contain micromolar to millimolar concentrations of Zn(2+), and disturbing lysosomal Zn(2+) homeostasis via lysosomal Zn(2+) release leads to mitochondria damage and consequent lytic cell death. Methods have been developed to image cellular Zn(2+) dynamics. Here, we present a protocol using GZnP3, a genetically encoded fluorescent Zn(2+) indicator, to assess lysosomal Zn(2+) release in cultured cells by fluorescence microscopy imaging. For complete details on the use and execution of this protocol, please refer to Du et al. (2021) or Minckley et al. (2019).
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spelling pubmed-91896252022-06-14 A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator Gu, Mingxue Hu, Meiqin Minckley, Taylor Pinchi, Prateeksunder Xu, Haoxing Qin, Yan Du, Wanlu STAR Protoc Protocol Intracellular vesicles such as lysosomes contain micromolar to millimolar concentrations of Zn(2+), and disturbing lysosomal Zn(2+) homeostasis via lysosomal Zn(2+) release leads to mitochondria damage and consequent lytic cell death. Methods have been developed to image cellular Zn(2+) dynamics. Here, we present a protocol using GZnP3, a genetically encoded fluorescent Zn(2+) indicator, to assess lysosomal Zn(2+) release in cultured cells by fluorescence microscopy imaging. For complete details on the use and execution of this protocol, please refer to Du et al. (2021) or Minckley et al. (2019). Elsevier 2022-06-10 /pmc/articles/PMC9189625/ /pubmed/35707683 http://dx.doi.org/10.1016/j.xpro.2022.101453 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gu, Mingxue
Hu, Meiqin
Minckley, Taylor
Pinchi, Prateeksunder
Xu, Haoxing
Qin, Yan
Du, Wanlu
A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title_full A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title_fullStr A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title_full_unstemmed A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title_short A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator
title_sort protocol to measure lysosomal zn(2+) release through a genetically encoded zn(2+) indicator
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189625/
https://www.ncbi.nlm.nih.gov/pubmed/35707683
http://dx.doi.org/10.1016/j.xpro.2022.101453
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