A protocol to measure lysosomal Zn(2+) release through a genetically encoded Zn(2+) indicator

Intracellular vesicles such as lysosomes contain micromolar to millimolar concentrations of Zn(2+), and disturbing lysosomal Zn(2+) homeostasis via lysosomal Zn(2+) release leads to mitochondria damage and consequent lytic cell death. Methods have been developed to image cellular Zn(2+) dynamics. He...

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Detalles Bibliográficos
Autores principales: Gu, Mingxue, Hu, Meiqin, Minckley, Taylor, Pinchi, Prateeksunder, Xu, Haoxing, Qin, Yan, Du, Wanlu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189625/
https://www.ncbi.nlm.nih.gov/pubmed/35707683
http://dx.doi.org/10.1016/j.xpro.2022.101453

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