CRISPR-Cas9 bends and twists DNA to read its sequence

In bacterial defense and genome editing applications, the CRISPR-associated protein Cas9 searches millions of DNA base pairs to locate a 20-nucleotide, guide-RNA-complementary target sequence that abuts a protospacer-adjacent motif (PAM). Target capture requires Cas9 to unwind DNA at candidate seque...

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Detalles Bibliográficos
Autores principales: Cofsky, Joshua C., Soczek, Katarzyna M., Knott, Gavin J., Nogales, Eva, Doudna, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189902/
https://www.ncbi.nlm.nih.gov/pubmed/35422516
http://dx.doi.org/10.1038/s41594-022-00756-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189902/
https://www.ncbi.nlm.nih.gov/pubmed/35422516
http://dx.doi.org/10.1038/s41594-022-00756-0

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