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Nanoscale resolution of microbial fiber degradation in action

The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehe...

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Autores principales: Tatli, Meltem, Moraïs, Sarah, Tovar-Herrera, Omar E, Bomble, Yannick J, Bayer, Edward A, Medalia, Ohad, Mizrahi, Itzhak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9191890/
https://www.ncbi.nlm.nih.gov/pubmed/35638899
http://dx.doi.org/10.7554/eLife.76523
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author Tatli, Meltem
Moraïs, Sarah
Tovar-Herrera, Omar E
Bomble, Yannick J
Bayer, Edward A
Medalia, Ohad
Mizrahi, Itzhak
author_facet Tatli, Meltem
Moraïs, Sarah
Tovar-Herrera, Omar E
Bomble, Yannick J
Bayer, Edward A
Medalia, Ohad
Mizrahi, Itzhak
author_sort Tatli, Meltem
collection PubMed
description The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form ‘cellulosome capsules’ driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intrapopulation interactions as key to understanding rates of fiber degradation.
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spelling pubmed-91918902022-06-14 Nanoscale resolution of microbial fiber degradation in action Tatli, Meltem Moraïs, Sarah Tovar-Herrera, Omar E Bomble, Yannick J Bayer, Edward A Medalia, Ohad Mizrahi, Itzhak eLife Microbiology and Infectious Disease The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form ‘cellulosome capsules’ driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intrapopulation interactions as key to understanding rates of fiber degradation. eLife Sciences Publications, Ltd 2022-05-31 /pmc/articles/PMC9191890/ /pubmed/35638899 http://dx.doi.org/10.7554/eLife.76523 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Microbiology and Infectious Disease
Tatli, Meltem
Moraïs, Sarah
Tovar-Herrera, Omar E
Bomble, Yannick J
Bayer, Edward A
Medalia, Ohad
Mizrahi, Itzhak
Nanoscale resolution of microbial fiber degradation in action
title Nanoscale resolution of microbial fiber degradation in action
title_full Nanoscale resolution of microbial fiber degradation in action
title_fullStr Nanoscale resolution of microbial fiber degradation in action
title_full_unstemmed Nanoscale resolution of microbial fiber degradation in action
title_short Nanoscale resolution of microbial fiber degradation in action
title_sort nanoscale resolution of microbial fiber degradation in action
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9191890/
https://www.ncbi.nlm.nih.gov/pubmed/35638899
http://dx.doi.org/10.7554/eLife.76523
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