Cargando…
Nanoscale resolution of microbial fiber degradation in action
The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehe...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9191890/ https://www.ncbi.nlm.nih.gov/pubmed/35638899 http://dx.doi.org/10.7554/eLife.76523 |
_version_ | 1784726115509600256 |
---|---|
author | Tatli, Meltem Moraïs, Sarah Tovar-Herrera, Omar E Bomble, Yannick J Bayer, Edward A Medalia, Ohad Mizrahi, Itzhak |
author_facet | Tatli, Meltem Moraïs, Sarah Tovar-Herrera, Omar E Bomble, Yannick J Bayer, Edward A Medalia, Ohad Mizrahi, Itzhak |
author_sort | Tatli, Meltem |
collection | PubMed |
description | The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form ‘cellulosome capsules’ driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intrapopulation interactions as key to understanding rates of fiber degradation. |
format | Online Article Text |
id | pubmed-9191890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-91918902022-06-14 Nanoscale resolution of microbial fiber degradation in action Tatli, Meltem Moraïs, Sarah Tovar-Herrera, Omar E Bomble, Yannick J Bayer, Edward A Medalia, Ohad Mizrahi, Itzhak eLife Microbiology and Infectious Disease The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form ‘cellulosome capsules’ driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intrapopulation interactions as key to understanding rates of fiber degradation. eLife Sciences Publications, Ltd 2022-05-31 /pmc/articles/PMC9191890/ /pubmed/35638899 http://dx.doi.org/10.7554/eLife.76523 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Microbiology and Infectious Disease Tatli, Meltem Moraïs, Sarah Tovar-Herrera, Omar E Bomble, Yannick J Bayer, Edward A Medalia, Ohad Mizrahi, Itzhak Nanoscale resolution of microbial fiber degradation in action |
title | Nanoscale resolution of microbial fiber degradation in action |
title_full | Nanoscale resolution of microbial fiber degradation in action |
title_fullStr | Nanoscale resolution of microbial fiber degradation in action |
title_full_unstemmed | Nanoscale resolution of microbial fiber degradation in action |
title_short | Nanoscale resolution of microbial fiber degradation in action |
title_sort | nanoscale resolution of microbial fiber degradation in action |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9191890/ https://www.ncbi.nlm.nih.gov/pubmed/35638899 http://dx.doi.org/10.7554/eLife.76523 |
work_keys_str_mv | AT tatlimeltem nanoscaleresolutionofmicrobialfiberdegradationinaction AT moraissarah nanoscaleresolutionofmicrobialfiberdegradationinaction AT tovarherreraomare nanoscaleresolutionofmicrobialfiberdegradationinaction AT bombleyannickj nanoscaleresolutionofmicrobialfiberdegradationinaction AT bayeredwarda nanoscaleresolutionofmicrobialfiberdegradationinaction AT medaliaohad nanoscaleresolutionofmicrobialfiberdegradationinaction AT mizrahiitzhak nanoscaleresolutionofmicrobialfiberdegradationinaction |