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Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction

BACKGROUND: Dental/oral diseases are one of the significant public health problems globally. Herbal medicines for managing oral diseases are considered an effective alternative to synthetic compounds due to their lower side effect. Azadirachta indica, Terminalia chebula, Camellia sinensis, and Piper...

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Autores principales: Assiry, Ali A., Bhavikatti, Shaeesta Khaleelahmed, Althobaiti, Fahad A., Mohamed, Roshan Noor, Karobari, Mohmed Isaqali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192215/
https://www.ncbi.nlm.nih.gov/pubmed/35707383
http://dx.doi.org/10.1155/2022/5870443
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author Assiry, Ali A.
Bhavikatti, Shaeesta Khaleelahmed
Althobaiti, Fahad A.
Mohamed, Roshan Noor
Karobari, Mohmed Isaqali
author_facet Assiry, Ali A.
Bhavikatti, Shaeesta Khaleelahmed
Althobaiti, Fahad A.
Mohamed, Roshan Noor
Karobari, Mohmed Isaqali
author_sort Assiry, Ali A.
collection PubMed
description BACKGROUND: Dental/oral diseases are one of the significant public health problems globally. Herbal medicines for managing oral diseases are considered an effective alternative to synthetic compounds due to their lower side effect. Azadirachta indica, Terminalia chebula, Camellia sinensis, and Piper nigrum are used to control and prevent oral inflammations in dentistry. In this study, we have evaluated the protease inhibition activity of these plant extracts, and further, the binding mode of the active ingredient of these plants with trypsin was studied using molecular docking. METHODS: In this study, protease inhibition activity was carried out using aqueous extracts of the plant parts such as Azadirachta indica (neem) twig, Terminalia chebula (Haritaki) fruit, Camellia sinensis (green tea) powder, and Piper nigrum (kali miri) seed. Next, to explore the binding mode of active ingredients azadirachtin, chebuligenic acid, catechin, and piperine with trypsin, we employed a molecular docking study using AutoDock4.2. RESULTS: The results revealed that the Azadirachta indica plant extract showed an IC50 value of 96.19 μg mL(−1), Camellia sinensis IC50 value of 188.50 μg mL(−1), Piper nigrum IC50 value of 371.20 μg mL(−1), and Terminalia chebula IC50 value of 639.48 μg mL(−1), when compared with standard drug diclofenac sodium, had IC50 value 93.00 μg mL(−1). Further, the docking result reveals that all the main active ingredients of these plants have significant binding affinity and prefer the same binding pocket of trypsin. CONCLUSION: Hence, our results show the importance of traditional plants Azadirachta indica, Terminalia chebula, green tea, and Piper nigrum to control oral disease conditions. As they show significant protease inhibition activity, hence, the active ingredient could act as a potential anti-inflammatory agent and further help to prevent or control oral disease conditions such as gingivitis and periodontitis.
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spelling pubmed-91922152022-06-14 Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction Assiry, Ali A. Bhavikatti, Shaeesta Khaleelahmed Althobaiti, Fahad A. Mohamed, Roshan Noor Karobari, Mohmed Isaqali Biomed Res Int Research Article BACKGROUND: Dental/oral diseases are one of the significant public health problems globally. Herbal medicines for managing oral diseases are considered an effective alternative to synthetic compounds due to their lower side effect. Azadirachta indica, Terminalia chebula, Camellia sinensis, and Piper nigrum are used to control and prevent oral inflammations in dentistry. In this study, we have evaluated the protease inhibition activity of these plant extracts, and further, the binding mode of the active ingredient of these plants with trypsin was studied using molecular docking. METHODS: In this study, protease inhibition activity was carried out using aqueous extracts of the plant parts such as Azadirachta indica (neem) twig, Terminalia chebula (Haritaki) fruit, Camellia sinensis (green tea) powder, and Piper nigrum (kali miri) seed. Next, to explore the binding mode of active ingredients azadirachtin, chebuligenic acid, catechin, and piperine with trypsin, we employed a molecular docking study using AutoDock4.2. RESULTS: The results revealed that the Azadirachta indica plant extract showed an IC50 value of 96.19 μg mL(−1), Camellia sinensis IC50 value of 188.50 μg mL(−1), Piper nigrum IC50 value of 371.20 μg mL(−1), and Terminalia chebula IC50 value of 639.48 μg mL(−1), when compared with standard drug diclofenac sodium, had IC50 value 93.00 μg mL(−1). Further, the docking result reveals that all the main active ingredients of these plants have significant binding affinity and prefer the same binding pocket of trypsin. CONCLUSION: Hence, our results show the importance of traditional plants Azadirachta indica, Terminalia chebula, green tea, and Piper nigrum to control oral disease conditions. As they show significant protease inhibition activity, hence, the active ingredient could act as a potential anti-inflammatory agent and further help to prevent or control oral disease conditions such as gingivitis and periodontitis. Hindawi 2022-06-06 /pmc/articles/PMC9192215/ /pubmed/35707383 http://dx.doi.org/10.1155/2022/5870443 Text en Copyright © 2022 Ali A. Assiry et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Assiry, Ali A.
Bhavikatti, Shaeesta Khaleelahmed
Althobaiti, Fahad A.
Mohamed, Roshan Noor
Karobari, Mohmed Isaqali
Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title_full Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title_fullStr Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title_full_unstemmed Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title_short Evaluation of In Vitro Antiprotease Activity of Selected Traditional Medicinal Herbs in Dentistry and Its In Silico PASS Prediction
title_sort evaluation of in vitro antiprotease activity of selected traditional medicinal herbs in dentistry and its in silico pass prediction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192215/
https://www.ncbi.nlm.nih.gov/pubmed/35707383
http://dx.doi.org/10.1155/2022/5870443
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