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Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein
OBJECTIVES: Lutein plays a critical role in the visual and cognitive development of infants. However, the application of lutein as a potential nutraceutical is limited by its low stability and poor water solubility. While various encapsulation systems have been developed for lutein to enhance its st...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193434/ http://dx.doi.org/10.1093/cdn/nzac049.015 |
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author | Zhang, Yanqi Kong, Lingyan Tan, Libo |
author_facet | Zhang, Yanqi Kong, Lingyan Tan, Libo |
author_sort | Zhang, Yanqi |
collection | PubMed |
description | OBJECTIVES: Lutein plays a critical role in the visual and cognitive development of infants. However, the application of lutein as a potential nutraceutical is limited by its low stability and poor water solubility. While various encapsulation systems have been developed for lutein to enhance its stability and bioavailability, few utilized bio-based polymers that are safe to use in infant foods. The aim of the study was to develop a novel emulsion system for lutein using food-grade colloids, octenylsuccinylated (OS) starch and gum Arabic (GA), as emulsifiers, which could improve the stability and bioaccessibility of lutein. METHODS: Lutein oil-in-water emulsions were prepared using two types of OS starch, capsule TA® (CTA) and HI-CAP®100 (HC), and one type of GA, TICAmulsion® 3020 (TM). Lutein was dissolved in olive oil and mixed with the aqueous biopolymer dispersions at 70% oil volume fraction using a homogenizer. The stabilities of the emulsion were assessed by measuring droplet size and distribution, changes of droplet size, and lutein retention at 25 and 45°C after a week of storage. The in vitro bioaccessibility of lutein was measured using a simulated in vitro gastrointestinal model. Free lutein was used as control. RESULTS: The mean droplet size of lutein emulsions stabilized by CTA, HC, and TM were 1.19 ± 0.75, 1.45 ± 0.80, and 1.18 ± 0.8 μm, respectively. After a week of storage at 25°C, the particle size stabilized by OS starches did not change significantly, while GA-stabilized emulsion showed 1.58-fold larger droplet size than fresh sample (P < 0.05). Lutein retention in the control and emulsions stabilized by CTA, HC, and TM were 79%, 88%, 89%, and 86% at day 7, respectively. After a week of storage at 45°C, the emulsions stabilized by CTA, HC, and TM showed 1.34-, 2.38-, and 1.55- fold larger particle size compared to fresh samples (P < 0.05). The retention of lutein in free lutein and emulsions were 78%, 86%, 46%, and 63%, respectively. The in vitro bioaccessibility of lutein emulsions were 1.95-, 1.46-, and 1.27- fold higher than that of free lutein (P < 0.05). CONCLUSIONS: Lutein emulsion stabilized by OS starch CTA had the best overall stability in droplet aggregation, color retention, and in vitro release. The oil-in-water emulsion stabilized by biopolymers could be promising carriers for lutein to expand their application in infant foods. FUNDING SOURCES: Louis/Evelyn Knol Fund. |
format | Online Article Text |
id | pubmed-9193434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-91934342022-06-14 Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein Zhang, Yanqi Kong, Lingyan Tan, Libo Curr Dev Nutr Carotenoids and Retinoids OBJECTIVES: Lutein plays a critical role in the visual and cognitive development of infants. However, the application of lutein as a potential nutraceutical is limited by its low stability and poor water solubility. While various encapsulation systems have been developed for lutein to enhance its stability and bioavailability, few utilized bio-based polymers that are safe to use in infant foods. The aim of the study was to develop a novel emulsion system for lutein using food-grade colloids, octenylsuccinylated (OS) starch and gum Arabic (GA), as emulsifiers, which could improve the stability and bioaccessibility of lutein. METHODS: Lutein oil-in-water emulsions were prepared using two types of OS starch, capsule TA® (CTA) and HI-CAP®100 (HC), and one type of GA, TICAmulsion® 3020 (TM). Lutein was dissolved in olive oil and mixed with the aqueous biopolymer dispersions at 70% oil volume fraction using a homogenizer. The stabilities of the emulsion were assessed by measuring droplet size and distribution, changes of droplet size, and lutein retention at 25 and 45°C after a week of storage. The in vitro bioaccessibility of lutein was measured using a simulated in vitro gastrointestinal model. Free lutein was used as control. RESULTS: The mean droplet size of lutein emulsions stabilized by CTA, HC, and TM were 1.19 ± 0.75, 1.45 ± 0.80, and 1.18 ± 0.8 μm, respectively. After a week of storage at 25°C, the particle size stabilized by OS starches did not change significantly, while GA-stabilized emulsion showed 1.58-fold larger droplet size than fresh sample (P < 0.05). Lutein retention in the control and emulsions stabilized by CTA, HC, and TM were 79%, 88%, 89%, and 86% at day 7, respectively. After a week of storage at 45°C, the emulsions stabilized by CTA, HC, and TM showed 1.34-, 2.38-, and 1.55- fold larger particle size compared to fresh samples (P < 0.05). The retention of lutein in free lutein and emulsions were 78%, 86%, 46%, and 63%, respectively. The in vitro bioaccessibility of lutein emulsions were 1.95-, 1.46-, and 1.27- fold higher than that of free lutein (P < 0.05). CONCLUSIONS: Lutein emulsion stabilized by OS starch CTA had the best overall stability in droplet aggregation, color retention, and in vitro release. The oil-in-water emulsion stabilized by biopolymers could be promising carriers for lutein to expand their application in infant foods. FUNDING SOURCES: Louis/Evelyn Knol Fund. Oxford University Press 2022-06-14 /pmc/articles/PMC9193434/ http://dx.doi.org/10.1093/cdn/nzac049.015 Text en © The Author 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Carotenoids and Retinoids Zhang, Yanqi Kong, Lingyan Tan, Libo Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title | Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title_full | Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title_fullStr | Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title_full_unstemmed | Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title_short | Biopolymer Stabilized Emulsions Improved Storage Stability and In Vitro Bioaccessibility of Lutein |
title_sort | biopolymer stabilized emulsions improved storage stability and in vitro bioaccessibility of lutein |
topic | Carotenoids and Retinoids |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193434/ http://dx.doi.org/10.1093/cdn/nzac049.015 |
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