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Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes

OBJECTIVES: According to the CDC, 38% of the adult US population have prediabetes. Previous studies reported a reduced abundance of butyrate producing bacteria in the colon of participants with prediabetes, potentially contributing to the progression to Type 2 Diabetes. We now studied whether the wh...

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Autores principales: Knezek, Savanah, Kirschner, Sarah, Engelen, Marielle, Deutz, Nicolaas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193871/
http://dx.doi.org/10.1093/cdn/nzac047.035
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author Knezek, Savanah
Kirschner, Sarah
Engelen, Marielle
Deutz, Nicolaas
author_facet Knezek, Savanah
Kirschner, Sarah
Engelen, Marielle
Deutz, Nicolaas
author_sort Knezek, Savanah
collection PubMed
description OBJECTIVES: According to the CDC, 38% of the adult US population have prediabetes. Previous studies reported a reduced abundance of butyrate producing bacteria in the colon of participants with prediabetes, potentially contributing to the progression to Type 2 Diabetes. We now studied whether the whole body production of short-chain fatty acids (SCFA: acetate, propionate, butyrate) are altered in middle aged/older adults with prediabetes. METHODS: We measured SCFA kinetics in 12 prediabetic (fasting glucose: 5.7–6.5 mM) and 25 healthy (fasting glucose < 5.7 mM) subjects, 40 years or older. In the fasted state, a pulse containing acetate[(13)C(2)], propionate[1-(13)C] and butyrate[1-13C] was administered intravenously while blood was sampled 4 times over a 30 min period. We measured tracer enrichments in plasma by GC-MS, whole body SCFA production from the area under the curve (AUC) and statistics by ANCOVA corrected for age, sex, and lean body mass as covariates with significance set at P< 0.05 and results expressed as means [95% CI]. RESULTS: The prediabetic subjects had a lower butyrate WBP (362 [270, 454] vs. 534 [403, 665] μmol/h, P = 0.021) while acetate WBP (18996 [15270, 23631] vs. 17431 [13478, 22544] μmol/h, P = 0.6049) and propionate WBP (1250 [1041, 1459] vs. 1322 [1053, 1590] μmol/h, P = 0.6624) were comparable. Plasma SCFA concentrations did not differ between the groups (all P >0.05). CONCLUSIONS: The reduced whole body production of butyrate observed in the prediabetic middle aged/older adults is probably related to the altered colonic bacterial composition. More research is required whether specific nutritional modulation of the colonic bacterial composition (e.g. fiber supplementation) is able to restore the butyrate WBP and can improve glucose control in adults with prediabetes. FUNDING SOURCES: None
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spelling pubmed-91938712022-06-14 Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes Knezek, Savanah Kirschner, Sarah Engelen, Marielle Deutz, Nicolaas Curr Dev Nutr Aging and Chronic Disease OBJECTIVES: According to the CDC, 38% of the adult US population have prediabetes. Previous studies reported a reduced abundance of butyrate producing bacteria in the colon of participants with prediabetes, potentially contributing to the progression to Type 2 Diabetes. We now studied whether the whole body production of short-chain fatty acids (SCFA: acetate, propionate, butyrate) are altered in middle aged/older adults with prediabetes. METHODS: We measured SCFA kinetics in 12 prediabetic (fasting glucose: 5.7–6.5 mM) and 25 healthy (fasting glucose < 5.7 mM) subjects, 40 years or older. In the fasted state, a pulse containing acetate[(13)C(2)], propionate[1-(13)C] and butyrate[1-13C] was administered intravenously while blood was sampled 4 times over a 30 min period. We measured tracer enrichments in plasma by GC-MS, whole body SCFA production from the area under the curve (AUC) and statistics by ANCOVA corrected for age, sex, and lean body mass as covariates with significance set at P< 0.05 and results expressed as means [95% CI]. RESULTS: The prediabetic subjects had a lower butyrate WBP (362 [270, 454] vs. 534 [403, 665] μmol/h, P = 0.021) while acetate WBP (18996 [15270, 23631] vs. 17431 [13478, 22544] μmol/h, P = 0.6049) and propionate WBP (1250 [1041, 1459] vs. 1322 [1053, 1590] μmol/h, P = 0.6624) were comparable. Plasma SCFA concentrations did not differ between the groups (all P >0.05). CONCLUSIONS: The reduced whole body production of butyrate observed in the prediabetic middle aged/older adults is probably related to the altered colonic bacterial composition. More research is required whether specific nutritional modulation of the colonic bacterial composition (e.g. fiber supplementation) is able to restore the butyrate WBP and can improve glucose control in adults with prediabetes. FUNDING SOURCES: None Oxford University Press 2022-06-14 /pmc/articles/PMC9193871/ http://dx.doi.org/10.1093/cdn/nzac047.035 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the American Society for Nutrition. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Aging and Chronic Disease
Knezek, Savanah
Kirschner, Sarah
Engelen, Marielle
Deutz, Nicolaas
Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title_full Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title_fullStr Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title_full_unstemmed Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title_short Whole Body Production of Butyrate, but Not of Acetate or Propionate, Is Reduced in Adults With Prediabetes
title_sort whole body production of butyrate, but not of acetate or propionate, is reduced in adults with prediabetes
topic Aging and Chronic Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193871/
http://dx.doi.org/10.1093/cdn/nzac047.035
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