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Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana

OBJECTIVES: The International Lipid-Based Nutrient Supplement (iLiNS) Project developed small-quantity lipid-based nutrient supplements (SQ-LNS) for mothers and their children with the goal of reducing malnutrition. The aim of this secondary outcome analysis is to investigate the effects of SQ-LNS o...

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Autores principales: Hong, Brian, Zheng, Jingyuan, Agus, Joanne, Adu-Afarwuah, Seth, Dewey, Kathryn, Zivkovic, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193990/
http://dx.doi.org/10.1093/cdn/nzac061.049
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author Hong, Brian
Zheng, Jingyuan
Agus, Joanne
Adu-Afarwuah, Seth
Dewey, Kathryn
Zivkovic, Angela
author_facet Hong, Brian
Zheng, Jingyuan
Agus, Joanne
Adu-Afarwuah, Seth
Dewey, Kathryn
Zivkovic, Angela
author_sort Hong, Brian
collection PubMed
description OBJECTIVES: The International Lipid-Based Nutrient Supplement (iLiNS) Project developed small-quantity lipid-based nutrient supplements (SQ-LNS) for mothers and their children with the goal of reducing malnutrition. The aim of this secondary outcome analysis is to investigate the effects of SQ-LNS on the activity of plasma lecithin-cholesterol acyltransferase (LCAT), a key enzyme involved in the remodeling of high-density lipoprotein (HDL) particles, which increases their cholesterol carrying capacity. METHODS: Plasma samples from a subset of 197 out of 1320 women at 36-wk gestation enrolled in the iLiNS-DYAD trial in Ghana were analyzed. In the main cohort, women were enrolled during the second trimester (≤ 20 wk gestation) and randomly assigned to receive SQ-LNS, iron and folic acid (IFA), or a multiple micronutrient supplement. Women in this subset received either SQ-LNS or IFA. Plasma LCAT activity was measured using a commercial fluorometric kit and the non-transformed data was compared by Wilcoxon test (mean ± SD). LCAT activity is expressed as a ratio (390 nm/470 nm), where greater ratio indicates higher enzyme activity. We also explored whether LCAT activity differed by enrollment season (Dry, November–April; Wet, May-October) or was associated with biomarkers of inflammation. RESULTS: There was no significant difference in LCAT activity between the SQ-LNS and IFA groups (1.44 ± 0.17 vs. 1.41 ± 0.14, P = 0.24). Women enrolled during the wet season had higher LCAT activity than women enrolled during the dry season (1.47 ± 0.15 vs. 1.37 ± 0.14, P < 0.001), with no differences between intervention groups stratified by season (P > 0.05). LCAT activity was negatively correlated with inflammatory biomarkers α1-acid glycoprotein (R = −0.19, P = 0.007) and C-reactive protein (R = −0.28, P < 0.001) measured at 36-wk gestation. CONCLUSIONS: Plasma LCAT activity, as a measure of HDL maturation and cholesterol carrying capacity, is more sensitive to factors mediated by seasonal variation (e.g., infection, inflammation) than by nutrient supplementation in this cohort of women in Ghana. These findings confirm a previously established link between HDL and inflammation. FUNDING SOURCES: This project was supported by Bill & Melinda Gates Foundation grant (OPP124589) to the University of California, Davis.
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spelling pubmed-91939902022-06-14 Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana Hong, Brian Zheng, Jingyuan Agus, Joanne Adu-Afarwuah, Seth Dewey, Kathryn Zivkovic, Angela Curr Dev Nutr Maternal, Perinatal and Pediatric Nutrition OBJECTIVES: The International Lipid-Based Nutrient Supplement (iLiNS) Project developed small-quantity lipid-based nutrient supplements (SQ-LNS) for mothers and their children with the goal of reducing malnutrition. The aim of this secondary outcome analysis is to investigate the effects of SQ-LNS on the activity of plasma lecithin-cholesterol acyltransferase (LCAT), a key enzyme involved in the remodeling of high-density lipoprotein (HDL) particles, which increases their cholesterol carrying capacity. METHODS: Plasma samples from a subset of 197 out of 1320 women at 36-wk gestation enrolled in the iLiNS-DYAD trial in Ghana were analyzed. In the main cohort, women were enrolled during the second trimester (≤ 20 wk gestation) and randomly assigned to receive SQ-LNS, iron and folic acid (IFA), or a multiple micronutrient supplement. Women in this subset received either SQ-LNS or IFA. Plasma LCAT activity was measured using a commercial fluorometric kit and the non-transformed data was compared by Wilcoxon test (mean ± SD). LCAT activity is expressed as a ratio (390 nm/470 nm), where greater ratio indicates higher enzyme activity. We also explored whether LCAT activity differed by enrollment season (Dry, November–April; Wet, May-October) or was associated with biomarkers of inflammation. RESULTS: There was no significant difference in LCAT activity between the SQ-LNS and IFA groups (1.44 ± 0.17 vs. 1.41 ± 0.14, P = 0.24). Women enrolled during the wet season had higher LCAT activity than women enrolled during the dry season (1.47 ± 0.15 vs. 1.37 ± 0.14, P < 0.001), with no differences between intervention groups stratified by season (P > 0.05). LCAT activity was negatively correlated with inflammatory biomarkers α1-acid glycoprotein (R = −0.19, P = 0.007) and C-reactive protein (R = −0.28, P < 0.001) measured at 36-wk gestation. CONCLUSIONS: Plasma LCAT activity, as a measure of HDL maturation and cholesterol carrying capacity, is more sensitive to factors mediated by seasonal variation (e.g., infection, inflammation) than by nutrient supplementation in this cohort of women in Ghana. These findings confirm a previously established link between HDL and inflammation. FUNDING SOURCES: This project was supported by Bill & Melinda Gates Foundation grant (OPP124589) to the University of California, Davis. Oxford University Press 2022-06-14 /pmc/articles/PMC9193990/ http://dx.doi.org/10.1093/cdn/nzac061.049 Text en © The Author 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Maternal, Perinatal and Pediatric Nutrition
Hong, Brian
Zheng, Jingyuan
Agus, Joanne
Adu-Afarwuah, Seth
Dewey, Kathryn
Zivkovic, Angela
Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title_full Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title_fullStr Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title_full_unstemmed Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title_short Plasma Lecithin-Cholesterol Acyltransferase Activity in Pregnant Women Enrolled in the iLiNS-DYAD Trial in Ghana
title_sort plasma lecithin-cholesterol acyltransferase activity in pregnant women enrolled in the ilins-dyad trial in ghana
topic Maternal, Perinatal and Pediatric Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193990/
http://dx.doi.org/10.1093/cdn/nzac061.049
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