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Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection

The lack of accessible noninvasive tools to examine the molecular alterations limits our understanding of the causes of total anomalous pulmonary venous connection (TAPVC), as well as the identification of effective operational strategies. Here, we consecutively enrolled peripheral leukocyte transcr...

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Autores principales: Wan, Zunmin, Li, Xiaohong, Sun, Jinghua, Li, Xiaohua, Liu, Zhongzhen, Dong, Haojian, Zhou, Qing, Qiu, Hailong, Xu, Jinjin, Yang, Tingyu, Wang, Wen-Jing, Ou, Yanqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194086/
https://www.ncbi.nlm.nih.gov/pubmed/35711367
http://dx.doi.org/10.3389/fcvm.2022.892000
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author Wan, Zunmin
Li, Xiaohong
Sun, Jinghua
Li, Xiaohua
Liu, Zhongzhen
Dong, Haojian
Zhou, Qing
Qiu, Hailong
Xu, Jinjin
Yang, Tingyu
Wang, Wen-Jing
Ou, Yanqiu
author_facet Wan, Zunmin
Li, Xiaohong
Sun, Jinghua
Li, Xiaohua
Liu, Zhongzhen
Dong, Haojian
Zhou, Qing
Qiu, Hailong
Xu, Jinjin
Yang, Tingyu
Wang, Wen-Jing
Ou, Yanqiu
author_sort Wan, Zunmin
collection PubMed
description The lack of accessible noninvasive tools to examine the molecular alterations limits our understanding of the causes of total anomalous pulmonary venous connection (TAPVC), as well as the identification of effective operational strategies. Here, we consecutively enrolled peripheral leukocyte transcripts of 26 preoperative obstructive and 22 non-obstructive patients with TAPVC. Two-hundred and fifty six differentially expressed mRNA and 27 differentially expressed long noncoding RNA transcripts were dysregulated. The up-regulated mRNA was enriched in the hydrogen peroxide catabolic process, response to mechanical stimulus, neutrophil degranulation, hemostasis, response to bacterium, and the NABA CORE MATRISOME pathway, all of which are associated with the development of fibrosis. Furthermore, we constructed predictive models using multiple machine-learning algorithms and tested the performance in the validation set. The mRNA NR3C2 and lncRNA MEG3 were screened based on multiple iterations. The random forest prediction model can predict preoperative obstruction patients in the validation set with high accuracy (area under curve = 1; sensitivity = 1). These data highlight the potential of peripheral leukocyte transcripts to evaluate obstructive-related pathophysiological alterations, leading to precision healthcare solutions that could improve patient survival after surgery. It also provides a novel direction for the study of preoperative obstructive TAPVC.
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spelling pubmed-91940862022-06-15 Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection Wan, Zunmin Li, Xiaohong Sun, Jinghua Li, Xiaohua Liu, Zhongzhen Dong, Haojian Zhou, Qing Qiu, Hailong Xu, Jinjin Yang, Tingyu Wang, Wen-Jing Ou, Yanqiu Front Cardiovasc Med Cardiovascular Medicine The lack of accessible noninvasive tools to examine the molecular alterations limits our understanding of the causes of total anomalous pulmonary venous connection (TAPVC), as well as the identification of effective operational strategies. Here, we consecutively enrolled peripheral leukocyte transcripts of 26 preoperative obstructive and 22 non-obstructive patients with TAPVC. Two-hundred and fifty six differentially expressed mRNA and 27 differentially expressed long noncoding RNA transcripts were dysregulated. The up-regulated mRNA was enriched in the hydrogen peroxide catabolic process, response to mechanical stimulus, neutrophil degranulation, hemostasis, response to bacterium, and the NABA CORE MATRISOME pathway, all of which are associated with the development of fibrosis. Furthermore, we constructed predictive models using multiple machine-learning algorithms and tested the performance in the validation set. The mRNA NR3C2 and lncRNA MEG3 were screened based on multiple iterations. The random forest prediction model can predict preoperative obstruction patients in the validation set with high accuracy (area under curve = 1; sensitivity = 1). These data highlight the potential of peripheral leukocyte transcripts to evaluate obstructive-related pathophysiological alterations, leading to precision healthcare solutions that could improve patient survival after surgery. It also provides a novel direction for the study of preoperative obstructive TAPVC. Frontiers Media S.A. 2022-05-31 /pmc/articles/PMC9194086/ /pubmed/35711367 http://dx.doi.org/10.3389/fcvm.2022.892000 Text en Copyright © 2022 Wan, Li, Sun, Li, Liu, Dong, Zhou, Qiu, Xu, Yang, Wang and Ou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Wan, Zunmin
Li, Xiaohong
Sun, Jinghua
Li, Xiaohua
Liu, Zhongzhen
Dong, Haojian
Zhou, Qing
Qiu, Hailong
Xu, Jinjin
Yang, Tingyu
Wang, Wen-Jing
Ou, Yanqiu
Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title_full Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title_fullStr Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title_full_unstemmed Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title_short Peripheral Blood Transcripts Predict Preoperative Obstructive Total Anomalous Pulmonary Venous Connection
title_sort peripheral blood transcripts predict preoperative obstructive total anomalous pulmonary venous connection
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194086/
https://www.ncbi.nlm.nih.gov/pubmed/35711367
http://dx.doi.org/10.3389/fcvm.2022.892000
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