Cargando…

Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation

OBJECTIVES: Increased dietary fiber intake is recommended as a potential gut microbiota-targeted nutritional strategy to reduce the risk of metabolic diseases. However, individuals’ gut microbiome composition can change differently in response to specific dietary fibers. The objective of this study...

Descripción completa

Detalles Bibliográficos
Autores principales: Dearakonda, Sri Lakshmi Sravani, Superdock, Dorothy, Ren, Jen, Poole, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194127/
http://dx.doi.org/10.1093/cdn/nzac069.009
_version_ 1784726643812597760
author Dearakonda, Sri Lakshmi Sravani
Superdock, Dorothy
Ren, Jen
Poole, Angela
author_facet Dearakonda, Sri Lakshmi Sravani
Superdock, Dorothy
Ren, Jen
Poole, Angela
author_sort Dearakonda, Sri Lakshmi Sravani
collection PubMed
description OBJECTIVES: Increased dietary fiber intake is recommended as a potential gut microbiota-targeted nutritional strategy to reduce the risk of metabolic diseases. However, individuals’ gut microbiome composition can change differently in response to specific dietary fibers. The objective of this study is to assess, through a resistant starch intervention, the factors that could be responsible for this heterogeneity in gut microbial alterations such as baseline gut microbiota and usual dietary fiber intake. METHODS: Healthy adults (n = 75) were enrolled in a 7.5-week randomized crossover dietary intervention study consisting of three 10-day treatment periods during which they consumed resistant starch type 2 (RS2), resistant starch type 4 (RS4), or a control digestible starch, in the form of crackers. The three treatment periods were separated by 5-day washouts with no cracker consumption. We performed 16S rRNA gene sequencing on fecal samples to characterize the gut microbiota, and measured fecal short-chain fatty acids (SCFA) and bile acids. We used QIIME2 to identify amplicon sequence variants (ASVs) in the 16S rRNA sequence data, MaAsLin2 to evaluate the effect of predictor variables on differential abundances of microbes, and linear mixed models to analyze changes in fecal SCFA and bile acids. RESULTS: We found significant changes in microbial composition after RS consumption but not after the control. Specifically, six ASVs were enriched including R. bromii and F. prausnitzii, which have been associated with butyrate production, after RS2 but not after RS4. Twelve ASVs increased after RS4, including Parabacteroides distasonis, which has been shown to be associated with protection against diseases like type 2 diabetes. There were no ASVs that significantly differed after the control. For all reported significant findings, q < 0.05. CONCLUSIONS: A short-term RS intervention resulted in RS type-specific gut microbial composition changes in healthy individuals. Ongoing analyses will further evaluate the predictors of gut microbiome changes in response to RS consumption by incorporating usual dietary intake, baseline gut microbiome composition, and host genetics. FUNDING SOURCES: NIH NIDDK T32 Award and the President's Council of Cornell Women.
format Online
Article
Text
id pubmed-9194127
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-91941272022-06-14 Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation Dearakonda, Sri Lakshmi Sravani Superdock, Dorothy Ren, Jen Poole, Angela Curr Dev Nutr Nutritional Microbiology/Microbiome OBJECTIVES: Increased dietary fiber intake is recommended as a potential gut microbiota-targeted nutritional strategy to reduce the risk of metabolic diseases. However, individuals’ gut microbiome composition can change differently in response to specific dietary fibers. The objective of this study is to assess, through a resistant starch intervention, the factors that could be responsible for this heterogeneity in gut microbial alterations such as baseline gut microbiota and usual dietary fiber intake. METHODS: Healthy adults (n = 75) were enrolled in a 7.5-week randomized crossover dietary intervention study consisting of three 10-day treatment periods during which they consumed resistant starch type 2 (RS2), resistant starch type 4 (RS4), or a control digestible starch, in the form of crackers. The three treatment periods were separated by 5-day washouts with no cracker consumption. We performed 16S rRNA gene sequencing on fecal samples to characterize the gut microbiota, and measured fecal short-chain fatty acids (SCFA) and bile acids. We used QIIME2 to identify amplicon sequence variants (ASVs) in the 16S rRNA sequence data, MaAsLin2 to evaluate the effect of predictor variables on differential abundances of microbes, and linear mixed models to analyze changes in fecal SCFA and bile acids. RESULTS: We found significant changes in microbial composition after RS consumption but not after the control. Specifically, six ASVs were enriched including R. bromii and F. prausnitzii, which have been associated with butyrate production, after RS2 but not after RS4. Twelve ASVs increased after RS4, including Parabacteroides distasonis, which has been shown to be associated with protection against diseases like type 2 diabetes. There were no ASVs that significantly differed after the control. For all reported significant findings, q < 0.05. CONCLUSIONS: A short-term RS intervention resulted in RS type-specific gut microbial composition changes in healthy individuals. Ongoing analyses will further evaluate the predictors of gut microbiome changes in response to RS consumption by incorporating usual dietary intake, baseline gut microbiome composition, and host genetics. FUNDING SOURCES: NIH NIDDK T32 Award and the President's Council of Cornell Women. Oxford University Press 2022-06-14 /pmc/articles/PMC9194127/ http://dx.doi.org/10.1093/cdn/nzac069.009 Text en © The Author 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nutritional Microbiology/Microbiome
Dearakonda, Sri Lakshmi Sravani
Superdock, Dorothy
Ren, Jen
Poole, Angela
Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title_full Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title_fullStr Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title_full_unstemmed Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title_short Understanding the Dynamics of Gut Microbiota Composition and Function Following Short-Term Resistant Starch Supplementation
title_sort understanding the dynamics of gut microbiota composition and function following short-term resistant starch supplementation
topic Nutritional Microbiology/Microbiome
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194127/
http://dx.doi.org/10.1093/cdn/nzac069.009
work_keys_str_mv AT dearakondasrilakshmisravani understandingthedynamicsofgutmicrobiotacompositionandfunctionfollowingshorttermresistantstarchsupplementation
AT superdockdorothy understandingthedynamicsofgutmicrobiotacompositionandfunctionfollowingshorttermresistantstarchsupplementation
AT renjen understandingthedynamicsofgutmicrobiotacompositionandfunctionfollowingshorttermresistantstarchsupplementation
AT pooleangela understandingthedynamicsofgutmicrobiotacompositionandfunctionfollowingshorttermresistantstarchsupplementation