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Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort

OBJECTIVES: The prevalence of cigarette smoking is 2 to 3 times greater among people living with HIV (PLWH) than in the HIV uninfected population. Smoking has also been associated with deleterious effects on the gut leading to potential microbial translocation. Thus, we sought to use CD14 (sCD14) as...

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Autores principales: Gouin, Angelique, Rodriguez, Jose Bastida, Camacho, Victoria, Lemoine, Maria, Huang, Yongjun, Jasmin, Jupshy, Martinez, Sabrina Sales, Seminario, Leslie, Tamargo, Javier, Sherman, Kenneth, Baum, Marianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194383/
http://dx.doi.org/10.1093/cdn/nzac069.013
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author Gouin, Angelique
Rodriguez, Jose Bastida
Camacho, Victoria
Lemoine, Maria
Huang, Yongjun
Jasmin, Jupshy
Martinez, Sabrina Sales
Seminario, Leslie
Tamargo, Javier
Sherman, Kenneth
Baum, Marianna
author_facet Gouin, Angelique
Rodriguez, Jose Bastida
Camacho, Victoria
Lemoine, Maria
Huang, Yongjun
Jasmin, Jupshy
Martinez, Sabrina Sales
Seminario, Leslie
Tamargo, Javier
Sherman, Kenneth
Baum, Marianna
author_sort Gouin, Angelique
collection PubMed
description OBJECTIVES: The prevalence of cigarette smoking is 2 to 3 times greater among people living with HIV (PLWH) than in the HIV uninfected population. Smoking has also been associated with deleterious effects on the gut leading to potential microbial translocation. Thus, we sought to use CD14 (sCD14) as a surrogate marker of microbial translocation to determine if smoking was associated with changes in microbial translocation. METHODS: A cross-sectional analysis was completed with participants from the Miami Adult Studies on HIV (MASH) cohort. Demographic characteristics and cigarette smoking status were self-reported. Levels of sCD14 were analyzed using ELISA kits in Dr. Sherman's laboratory. HIV serostatus and viral load (VL) were abstracted from medical records with the participants’ consent. Statistical analyses included descriptive statistics, T-test, linear regression for sCD14 levels, and logistic regression to calculate the odds of having an sCD14 level above the sample median (1064.3 ng/mL). All analyses were conducted on SPSS 26. RESULTS: A total of 470 participants were included in the analysis. The mean age was 53 ± 8 years, 42.8% were females and 64.5% were Black. PLWH accounted for 76.8% of participants and 85.1% had a suppressed VL (<50 copies/mL). Smokers had significantly higher mean sCD14 levels than non-smokers (1025.8 ± 429.0 vs. 1183.4 ± 465.7, respectively; P = 0.002). This relationship remained significant after adjusting for sex, age, BMI, and HIV status (β = 166.70, SE = 52.36, P = 0.002). Also, smoking tended to be associated with 1.6 times the odds of having high sCD14 levels, adjusted for BMI, age, sex, and HIV status (β = 1.6, 95% CI = 1.0–2.6, P = 0.051). CONCLUSIONS: Cigarette smoking appears to contribute to immune activation regards of HIV status which may contribute to microbial translocation, regardless of HIV status. The findings provide further evidence of the deleterious effect of cigarette smoking on physiological functions, including gut health. Further research is needed to determine the long-term effects of smoking on immune activation and microbial translocation. FUNDING SOURCES: Supported by the National Institute on Drug Abuse.
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spelling pubmed-91943832022-06-15 Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort Gouin, Angelique Rodriguez, Jose Bastida Camacho, Victoria Lemoine, Maria Huang, Yongjun Jasmin, Jupshy Martinez, Sabrina Sales Seminario, Leslie Tamargo, Javier Sherman, Kenneth Baum, Marianna Curr Dev Nutr Nutritional Microbiology/Microbiome OBJECTIVES: The prevalence of cigarette smoking is 2 to 3 times greater among people living with HIV (PLWH) than in the HIV uninfected population. Smoking has also been associated with deleterious effects on the gut leading to potential microbial translocation. Thus, we sought to use CD14 (sCD14) as a surrogate marker of microbial translocation to determine if smoking was associated with changes in microbial translocation. METHODS: A cross-sectional analysis was completed with participants from the Miami Adult Studies on HIV (MASH) cohort. Demographic characteristics and cigarette smoking status were self-reported. Levels of sCD14 were analyzed using ELISA kits in Dr. Sherman's laboratory. HIV serostatus and viral load (VL) were abstracted from medical records with the participants’ consent. Statistical analyses included descriptive statistics, T-test, linear regression for sCD14 levels, and logistic regression to calculate the odds of having an sCD14 level above the sample median (1064.3 ng/mL). All analyses were conducted on SPSS 26. RESULTS: A total of 470 participants were included in the analysis. The mean age was 53 ± 8 years, 42.8% were females and 64.5% were Black. PLWH accounted for 76.8% of participants and 85.1% had a suppressed VL (<50 copies/mL). Smokers had significantly higher mean sCD14 levels than non-smokers (1025.8 ± 429.0 vs. 1183.4 ± 465.7, respectively; P = 0.002). This relationship remained significant after adjusting for sex, age, BMI, and HIV status (β = 166.70, SE = 52.36, P = 0.002). Also, smoking tended to be associated with 1.6 times the odds of having high sCD14 levels, adjusted for BMI, age, sex, and HIV status (β = 1.6, 95% CI = 1.0–2.6, P = 0.051). CONCLUSIONS: Cigarette smoking appears to contribute to immune activation regards of HIV status which may contribute to microbial translocation, regardless of HIV status. The findings provide further evidence of the deleterious effect of cigarette smoking on physiological functions, including gut health. Further research is needed to determine the long-term effects of smoking on immune activation and microbial translocation. FUNDING SOURCES: Supported by the National Institute on Drug Abuse. Oxford University Press 2022-06-14 /pmc/articles/PMC9194383/ http://dx.doi.org/10.1093/cdn/nzac069.013 Text en © The Author 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nutritional Microbiology/Microbiome
Gouin, Angelique
Rodriguez, Jose Bastida
Camacho, Victoria
Lemoine, Maria
Huang, Yongjun
Jasmin, Jupshy
Martinez, Sabrina Sales
Seminario, Leslie
Tamargo, Javier
Sherman, Kenneth
Baum, Marianna
Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title_full Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title_fullStr Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title_full_unstemmed Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title_short Smoking Is Associated With Microbial Translocation in the Miami Adult Studies on HIV (MASH) Cohort
title_sort smoking is associated with microbial translocation in the miami adult studies on hiv (mash) cohort
topic Nutritional Microbiology/Microbiome
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194383/
http://dx.doi.org/10.1093/cdn/nzac069.013
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