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Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194656/ https://www.ncbi.nlm.nih.gov/pubmed/35711290 http://dx.doi.org/10.1016/j.mtbio.2022.100311 |
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author | Cao, Zhenping Wang, Lu Liu, Rui Lin, Sisi Wu, Feng Liu, Jinyao |
author_facet | Cao, Zhenping Wang, Lu Liu, Rui Lin, Sisi Wu, Feng Liu, Jinyao |
author_sort | Cao, Zhenping |
collection | PubMed |
description | The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepare living bacterial probes for real-time dynamic, dual-modal, and molecular oxygen-independent imaging of the host microbiota. Carrying FAST endows bacteria with rapid on-demand turn on-off fluorescence by adding or removal of corresponding fluorogens. Encoded bacteria are able to reversibly switch emission bands for dual-color fluorescence imaging via fluorogen exchange. Due to molecular oxygen-independent emission of FAST, encoded bacteria can emit fluorescence under anaerobic environments including the gut and tumor. These living probes demonstrate the applicability to quantify the vitality of bacteria transplanted to the gut microbiota. This work proposes a unique fluorescence probe for investigating the dynamics of the host microbiota. |
format | Online Article Text |
id | pubmed-9194656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91946562022-06-15 Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging Cao, Zhenping Wang, Lu Liu, Rui Lin, Sisi Wu, Feng Liu, Jinyao Mater Today Bio Living Materials edited by Chao Zhong The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepare living bacterial probes for real-time dynamic, dual-modal, and molecular oxygen-independent imaging of the host microbiota. Carrying FAST endows bacteria with rapid on-demand turn on-off fluorescence by adding or removal of corresponding fluorogens. Encoded bacteria are able to reversibly switch emission bands for dual-color fluorescence imaging via fluorogen exchange. Due to molecular oxygen-independent emission of FAST, encoded bacteria can emit fluorescence under anaerobic environments including the gut and tumor. These living probes demonstrate the applicability to quantify the vitality of bacteria transplanted to the gut microbiota. This work proposes a unique fluorescence probe for investigating the dynamics of the host microbiota. Elsevier 2022-06-06 /pmc/articles/PMC9194656/ /pubmed/35711290 http://dx.doi.org/10.1016/j.mtbio.2022.100311 Text en © 2022 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Living Materials edited by Chao Zhong Cao, Zhenping Wang, Lu Liu, Rui Lin, Sisi Wu, Feng Liu, Jinyao Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title | Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title_full | Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title_fullStr | Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title_full_unstemmed | Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title_short | Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
title_sort | encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging |
topic | Living Materials edited by Chao Zhong |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194656/ https://www.ncbi.nlm.nih.gov/pubmed/35711290 http://dx.doi.org/10.1016/j.mtbio.2022.100311 |
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