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Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging

The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepa...

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Autores principales: Cao, Zhenping, Wang, Lu, Liu, Rui, Lin, Sisi, Wu, Feng, Liu, Jinyao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194656/
https://www.ncbi.nlm.nih.gov/pubmed/35711290
http://dx.doi.org/10.1016/j.mtbio.2022.100311
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author Cao, Zhenping
Wang, Lu
Liu, Rui
Lin, Sisi
Wu, Feng
Liu, Jinyao
author_facet Cao, Zhenping
Wang, Lu
Liu, Rui
Lin, Sisi
Wu, Feng
Liu, Jinyao
author_sort Cao, Zhenping
collection PubMed
description The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepare living bacterial probes for real-time dynamic, dual-modal, and molecular oxygen-independent imaging of the host microbiota. Carrying FAST endows bacteria with rapid on-demand turn on-off fluorescence by adding or removal of corresponding fluorogens. Encoded bacteria are able to reversibly switch emission bands for dual-color fluorescence imaging via fluorogen exchange. Due to molecular oxygen-independent emission of FAST, encoded bacteria can emit fluorescence under anaerobic environments including the gut and tumor. These living probes demonstrate the applicability to quantify the vitality of bacteria transplanted to the gut microbiota. This work proposes a unique fluorescence probe for investigating the dynamics of the host microbiota.
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spelling pubmed-91946562022-06-15 Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging Cao, Zhenping Wang, Lu Liu, Rui Lin, Sisi Wu, Feng Liu, Jinyao Mater Today Bio Living Materials edited by Chao Zhong The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepare living bacterial probes for real-time dynamic, dual-modal, and molecular oxygen-independent imaging of the host microbiota. Carrying FAST endows bacteria with rapid on-demand turn on-off fluorescence by adding or removal of corresponding fluorogens. Encoded bacteria are able to reversibly switch emission bands for dual-color fluorescence imaging via fluorogen exchange. Due to molecular oxygen-independent emission of FAST, encoded bacteria can emit fluorescence under anaerobic environments including the gut and tumor. These living probes demonstrate the applicability to quantify the vitality of bacteria transplanted to the gut microbiota. This work proposes a unique fluorescence probe for investigating the dynamics of the host microbiota. Elsevier 2022-06-06 /pmc/articles/PMC9194656/ /pubmed/35711290 http://dx.doi.org/10.1016/j.mtbio.2022.100311 Text en © 2022 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Living Materials edited by Chao Zhong
Cao, Zhenping
Wang, Lu
Liu, Rui
Lin, Sisi
Wu, Feng
Liu, Jinyao
Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title_full Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title_fullStr Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title_full_unstemmed Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title_short Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
title_sort encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging
topic Living Materials edited by Chao Zhong
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194656/
https://www.ncbi.nlm.nih.gov/pubmed/35711290
http://dx.doi.org/10.1016/j.mtbio.2022.100311
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