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Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles

High transmembrane delivery efficiency of nanoparticles has attracted substantial interest for biomedical applications. It has been proved that the desired physicochemical properties of nanoparticles were efficient for obtaining a high cellular uptake capacity. On the other hand, biophysical stimuli...

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Autores principales: Huang, Siyuan, Su, Qi, Hou, Xiaoqiang, Han, Kuankuan, Ma, Shufang, Xu, Bingshe, Yang, Yingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194857/
https://www.ncbi.nlm.nih.gov/pubmed/35711638
http://dx.doi.org/10.3389/fbioe.2022.922159
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author Huang, Siyuan
Su, Qi
Hou, Xiaoqiang
Han, Kuankuan
Ma, Shufang
Xu, Bingshe
Yang, Yingjun
author_facet Huang, Siyuan
Su, Qi
Hou, Xiaoqiang
Han, Kuankuan
Ma, Shufang
Xu, Bingshe
Yang, Yingjun
author_sort Huang, Siyuan
collection PubMed
description High transmembrane delivery efficiency of nanoparticles has attracted substantial interest for biomedical applications. It has been proved that the desired physicochemical properties of nanoparticles were efficient for obtaining a high cellular uptake capacity. On the other hand, biophysical stimuli from in situ microenvironment were also indicated as another essential factor in the regulation of cellular uptake capacity. Unfortunately, the influence of colony morphology on cellular uptake capacity was rarely analyzed. In this study, micropatterned PDMS stencils containing circular holes of 800/1,200 μm in diameter were applied to control colonies’ size. The amino-modified nanoparticles were cocultured with micropatterned colonies to analyze the influence of colonies’ morphology on the cellular uptake capacity of nanoparticles. Consequently, more endocytosed nanoparticles in larger colonies were related with a bigger dose of nanoparticles within a larger area. Additionally, the high cell density decreased the membrane–nanoparticles’ contacting probability but enhanced clathrin-mediated endocytosis. With these contrary effects, the cells with medium cell density or located in the peripheral region of the micropatterned colonies showed a higher cellular uptake capacity of nanoparticles.
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spelling pubmed-91948572022-06-15 Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles Huang, Siyuan Su, Qi Hou, Xiaoqiang Han, Kuankuan Ma, Shufang Xu, Bingshe Yang, Yingjun Front Bioeng Biotechnol Bioengineering and Biotechnology High transmembrane delivery efficiency of nanoparticles has attracted substantial interest for biomedical applications. It has been proved that the desired physicochemical properties of nanoparticles were efficient for obtaining a high cellular uptake capacity. On the other hand, biophysical stimuli from in situ microenvironment were also indicated as another essential factor in the regulation of cellular uptake capacity. Unfortunately, the influence of colony morphology on cellular uptake capacity was rarely analyzed. In this study, micropatterned PDMS stencils containing circular holes of 800/1,200 μm in diameter were applied to control colonies’ size. The amino-modified nanoparticles were cocultured with micropatterned colonies to analyze the influence of colonies’ morphology on the cellular uptake capacity of nanoparticles. Consequently, more endocytosed nanoparticles in larger colonies were related with a bigger dose of nanoparticles within a larger area. Additionally, the high cell density decreased the membrane–nanoparticles’ contacting probability but enhanced clathrin-mediated endocytosis. With these contrary effects, the cells with medium cell density or located in the peripheral region of the micropatterned colonies showed a higher cellular uptake capacity of nanoparticles. Frontiers Media S.A. 2022-05-31 /pmc/articles/PMC9194857/ /pubmed/35711638 http://dx.doi.org/10.3389/fbioe.2022.922159 Text en Copyright © 2022 Huang, Su, Hou, Han, Ma, Xu and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Huang, Siyuan
Su, Qi
Hou, Xiaoqiang
Han, Kuankuan
Ma, Shufang
Xu, Bingshe
Yang, Yingjun
Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title_full Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title_fullStr Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title_full_unstemmed Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title_short Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles
title_sort influence of colonies’ morphological cues on cellular uptake capacity of nanoparticles
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194857/
https://www.ncbi.nlm.nih.gov/pubmed/35711638
http://dx.doi.org/10.3389/fbioe.2022.922159
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