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Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome

INTRODUCTION: To evaluate factors associated with successful comprehensive genomic sequencing of image-guided percutaneous needle biopsies in patients with lung cancer using a broad hybrid capture-based next-generation sequencing assay (CHCA). METHODS: We conducted a single-institution retrospective...

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Autores principales: Elsakka, Ahmed, Petre, Elena N., Ridouani, Fourat, Ghosn, Mario, Bott, Matthew J., Husta, Bryan C., Arcila, Maria E., Alexander, Erica, Solomon, Stephen B., Ziv, Etay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194869/
https://www.ncbi.nlm.nih.gov/pubmed/35711720
http://dx.doi.org/10.1016/j.jtocrr.2022.100342
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author Elsakka, Ahmed
Petre, Elena N.
Ridouani, Fourat
Ghosn, Mario
Bott, Matthew J.
Husta, Bryan C.
Arcila, Maria E.
Alexander, Erica
Solomon, Stephen B.
Ziv, Etay
author_facet Elsakka, Ahmed
Petre, Elena N.
Ridouani, Fourat
Ghosn, Mario
Bott, Matthew J.
Husta, Bryan C.
Arcila, Maria E.
Alexander, Erica
Solomon, Stephen B.
Ziv, Etay
author_sort Elsakka, Ahmed
collection PubMed
description INTRODUCTION: To evaluate factors associated with successful comprehensive genomic sequencing of image-guided percutaneous needle biopsies in patients with lung cancer using a broad hybrid capture-based next-generation sequencing assay (CHCA). METHODS: We conducted a single-institution retrospective review of image-guided percutaneous transthoracic needle biopsies from January 2018 to December 2019. Samples with confirmed diagnosis of primary lung cancer and for which CHCA had been attempted were identified. Pathologic, clinical data and results of the CHCA were reviewed. Covariates associated with CHCA success were tested for using Fisher’s exact test or Wilcoxon ranked sum test. Logistic regression was used to identify factors independently associated with likelihood of CHCA success. RESULTS: CHCA was requested for 479 samples and was successful for 433 (91%), with a median coverage depth of 659X. Factors independently associated with lower likelihood of CHCA success included small tumor size (OR = 0.26 [95% confidence interval (CI): 0.11–0.62, p = 0.002]), intraoperative inadequacy on cytologic assessment (OR = 0.18 [95% CI: 0.06–0.63, p = 0.005]), small caliber needles (≥20-gauge) (OR = 0.22 [95% CI: 0.10–0.45, p < 0.001]), and presence of lung parenchymal abnormalities (OR = 0.12 [95% CI: 0.05–0.25, p < 0.001]). Pneumothorax requiring chest tube insertion occurred in 6% of the procedures. No grade IV complications or procedure-related deaths were reported. CONCLUSIONS: Percutaneous image-guided transthoracic needle biopsy is safe and has 91% success rate for CHCA in primary lung cancer. Intraoperative inadequacy, small caliber needle, presence of parenchymal abnormalities, and small tumor size (≤1 cm) are independently associated with likelihood of failure.
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spelling pubmed-91948692022-06-15 Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome Elsakka, Ahmed Petre, Elena N. Ridouani, Fourat Ghosn, Mario Bott, Matthew J. Husta, Bryan C. Arcila, Maria E. Alexander, Erica Solomon, Stephen B. Ziv, Etay JTO Clin Res Rep Original Article INTRODUCTION: To evaluate factors associated with successful comprehensive genomic sequencing of image-guided percutaneous needle biopsies in patients with lung cancer using a broad hybrid capture-based next-generation sequencing assay (CHCA). METHODS: We conducted a single-institution retrospective review of image-guided percutaneous transthoracic needle biopsies from January 2018 to December 2019. Samples with confirmed diagnosis of primary lung cancer and for which CHCA had been attempted were identified. Pathologic, clinical data and results of the CHCA were reviewed. Covariates associated with CHCA success were tested for using Fisher’s exact test or Wilcoxon ranked sum test. Logistic regression was used to identify factors independently associated with likelihood of CHCA success. RESULTS: CHCA was requested for 479 samples and was successful for 433 (91%), with a median coverage depth of 659X. Factors independently associated with lower likelihood of CHCA success included small tumor size (OR = 0.26 [95% confidence interval (CI): 0.11–0.62, p = 0.002]), intraoperative inadequacy on cytologic assessment (OR = 0.18 [95% CI: 0.06–0.63, p = 0.005]), small caliber needles (≥20-gauge) (OR = 0.22 [95% CI: 0.10–0.45, p < 0.001]), and presence of lung parenchymal abnormalities (OR = 0.12 [95% CI: 0.05–0.25, p < 0.001]). Pneumothorax requiring chest tube insertion occurred in 6% of the procedures. No grade IV complications or procedure-related deaths were reported. CONCLUSIONS: Percutaneous image-guided transthoracic needle biopsy is safe and has 91% success rate for CHCA in primary lung cancer. Intraoperative inadequacy, small caliber needle, presence of parenchymal abnormalities, and small tumor size (≤1 cm) are independently associated with likelihood of failure. Elsevier 2022-05-18 /pmc/articles/PMC9194869/ /pubmed/35711720 http://dx.doi.org/10.1016/j.jtocrr.2022.100342 Text en © 2022 by the International Association for the Study of Lung Cancer. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Elsakka, Ahmed
Petre, Elena N.
Ridouani, Fourat
Ghosn, Mario
Bott, Matthew J.
Husta, Bryan C.
Arcila, Maria E.
Alexander, Erica
Solomon, Stephen B.
Ziv, Etay
Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title_full Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title_fullStr Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title_full_unstemmed Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title_short Percutaneous Image-Guided Biopsy for a Comprehensive Hybridization Capture-Based Next-Generation Sequencing in Primary Lung Cancer: Safety, Efficacy, and Predictors of Outcome
title_sort percutaneous image-guided biopsy for a comprehensive hybridization capture-based next-generation sequencing in primary lung cancer: safety, efficacy, and predictors of outcome
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194869/
https://www.ncbi.nlm.nih.gov/pubmed/35711720
http://dx.doi.org/10.1016/j.jtocrr.2022.100342
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